Optimization of condition for pectin extraction from pomelo peel using response surface methodology

Pham Van Hung * , Nguyen Duong Phuoc Tuan , Tan Hoang Nam and Nguyen Thi Lan Phi

* Corresponding author (pvhung@hcmiu.edu.vn)

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Received: 27 Oct 2019
Revised: 01 Mar 2020
Accepted: 31 Mar 2020
Published: 31 Mar 2020
DOI: 10.22144/ctu.jen.2020.007
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Pham, V. H., Nguyen, D. P. T., Tan, H. N., & Nguyen, T. L. P. (2020). Optimization of condition for pectin extraction from pomelo peel using response surface methodology. CTU Journal of Innovation and Sustainable Development , 12(1), 50-57. https://doi.org/10.22144/ctu.jen.2020.007
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Vol. 12 No. 1 (2020)

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Abstract

The objective of this study was to optimize extraction condition of pectin from the pomelo peel based on the ultrasound-assisted extraction method using response surface methodology. The effect of four independent variables: solid/liquid ratio (1/30, 1/40, 1/50 g/mL), pH values of citric acid (pH 1.5 - 2.5), sonication time (30 - 50 min) and extraction temperature (60 - 80oC) on the yield of pectin extracted from Da Xanh pomelo pomace was analyzed using Box-Behnken design. The high coefficient of determination value (R2 = 0.9299) indicated that the experimental data were fitted to a second order polynomial equation using multiple regression analysis. The model was highly significant because the model F-value was 11.37 with low p-value (p < 0.0001). Therefore, the model could be employed to optimize the extraction process. Optimal experimental extraction condition for the highest pectin yield from pomelo peel (12.4%) were obtained with the solid/liquid ratio of 1/49.5 g/mL, pH of citric acid of 1.5, sonication time of 47 min and temperature of 78oC. The results obtained from validation experiments were consistent with the predicted data.
Keywords: Pectin, pomelo peel, response surface methodology, ultrasound-assisted extraction

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