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The survey of plant diversity and vegetation identified in Vam Ho Bird Sanctuary and surrounding areas (Ba Tri and Binh Dai districts, Ben Tre province) was performed in October 2024. The study aimed to build the plant diversity basics for investing and upgrading Vam Ho to the status of a Natural Heritage site. Apply transect survey and sampling, the study recorded 179 species, belonging to 55 vascular plants. Among them, Azima sarmentosa, Oryza rufipogon, Psilotum nudum were listed in the Vietnam Red Book, Avicennia marina var. rumphiana, Phoenix paludosa, Sonneratia ovata, Ceriops decandra were on the IUCN Red List. In addition, a total of 155 out of 179 recorded species has belonged to at least one of six main use groups, medicine, food, animal/poultry feed, ornamental plants, timber/charcoal/construction materials, and household items/handicrafts. The vegetation was divided into three types, including the natural habitat in Vam Ho Bird Sanctuary, Casuarina equisetifolia coastal plantation and estuarine-coastal mangrove forests. The establishment of the Natural Heritage Site there would create the positive conditions for the activities of the habitat conservation (including waterbird) and coastal protection, which help to reduce the pressure of the environmental degradation for the Ba Lai estuary in the near future.