Lieu My Dong * and Dang Thi Kim Thuy

* Correspondence: Lieu My Dong (email:

Main Article Content


In this study, the antimicrobial activities of cassia oil (Cinnamomum cassia), acetic acid, and lactic acid, alone or in combination, against Escherichia coli ATCC 23631 were evaluated by the agar disk diffusion, broth dilution, and UV absorption determination methods. In the agar disk diffusion assays using the antibiotics ampicillin and nalidixic acid as the positive control, all three agents tested exhibited an effective inhibition against E. coli with inhibition zones ranging from 3.00 to 15.33 mm, compared to those of ampicillin and nalidixic acid ranging from 0.00 to 12.00 mm. In the broth dilution assays, the MBC (Minimum bactericidal concentration) values of cassia oil and organic acid were determined from 300 to 5.000 ppm in which cassia oil giving the best result. In both assays, acetic acid and lactic acid showed similar antimicrobial activities, which are lower than those of cassia oil or their combinations. The assays also suggested that the combinations of cassia oil and lactic acid showed a higher synergic effect than those of cassia oil and acetic acid. Although the antimicrobial activities of the combinations of cassia oil with the organic acids were not higher than using the pure oil, the use of these combinations would reduce the essential oil amount needed to inhibit E. coli.
Keywords: acetic acid, antimicrobial activity, cassia oil, Escherichia coli, lactic acid

Article Details


Aleksic, V. and Knezevic, P., 2014. Antimicrobial and antioxidative activity of extracts and essential oils of Myrtus communis L. Microbiological Research. 169(4): 240-254.

Chao, S. C., Young, D. G., and Oberg, C. J., 2000. Screening for inhibitory activity of essential oils on selected bacteria, fungi and viruses. Journal of Essential Oil Research. 12(5): 639-649.

Cui, H. Y., Zhou, H., Lin, L. et al., 2016. Antibacterial activity and mechanism of cinnamon essential oil and its application in milk. The Journal of Animal & Plant Sciences. 26(2): 532-541.

Del Nobile, M. A., Lucera, A., Costa, C. and Conte, A., 2012.. Food applications of natural antimicrobial compounds. Frontiers in microbiology. 3: 287.

Dong, L. M., and Thuy, D. T. K., 2019. Evaluation of the synergistic effect of ethanol and lemongrass oil against Aspergillus niger. Journal of Microbiology, Biotechnology & Food Sciences. 8(6): 1312-1316.

Friedly, E. C., Crandall, P. G., Ricke, S. C. et al., 2009. In vitro antilisterial effects of citrus oil fractions in combination with organic acids. Journal of Food Science. 74(2): M67-M72.

Gutierrez, J., Barry-Ryan, C., and Bourke, P., 2008. The antimicrobial efficacy of plant essential oil combinations and interactions with food ingredients. International Journal of Food Microbiology. 124(1): 91-97.

Jay, J. M., Loessner, M. J., and Golden, D. A., 2005. Radiation protection of foods, and nature of microbial radiation resistance. Modern Food Microbiology. Food Science Text Series. Springer, Boston, MA. 371-394

Karabagias, I., Badeka, A., and Kontominas, M. G., 2011. Shelf life extension of lamb meat using thyme or oregano essential oils and modified atmosphere packaging. Meat Science. 88(1): 109-116.

Lieu, D. M., Dang, T. T., and Nguyen, H. T., 2018. Enhance the anti-microorganism activity of cinnamon oil by xanthan gum as emulsifying agent. In AIP Conference Proceedings. AIP Publishing. 1954(1): 040017.

Marino, M., Bersani, C., and Comi, G., 2001. Impedance measurements to study the antimicrobial activity of essential oils from Lamiaceae and Compositae. International Journal of Food Microbiology. 67(3): 187-195.

Maziero, M. T., and de Oliveira, T. C. R., 2010. Effect of refrigeration and frozen storage on the Campylobacter jejuni recovery from naturally contaminated broiler carcasses. Brazilian Journal of Microbiology. 41(2): 501-505.

Ozogul, Y., Kuley, E., Ucar, Y., and Ozogul, F., 2015. Antimicrobial impacts of essential oils on food borne-pathogens. Recent patents on food, nutrition & agriculture. 7(1): 53-61.

Park, S. H., Choi, M. R., Park, J. W. et al., 2011 Use of organic acids to inactivate Escherichia coli O157, H7, Salmonella typhimurium, and Listeria monocytogenes on organic fresh apples and lettuce. J Food Sci. 76: 293–298.

Rahman, A., and Kang, S. C., 2009. In vitro control of food-borne and food spoilage bacteria by essential oil and ethanol extracts of Lonicera japonica Thunb. Food Chemistry. 116 (3): 670-675.

Sheeladevi, A., and Ramanathan, N., 2012. Antibacterial activity of plant essential oils against food borne bacteria. International Journal of Pharmaceutical & Biological Archives. 3(5): 1106-1109.

Škrinjar, M. M., and Nemet, N. T., 2009. Antimicrobial effects of spices and herbs essential oils. Acta Periodica Technologica. 40: 195-209.

Skřivanová, E., Marounek, M., Benda, V. et al., 2006. Susceptibility of Escherichia coli, Salmonella sp and Clostridium perfringens to organic acids and monolaurin. Veterinární Medicína. 51(3): 81-88.

Boubaker, H., Karim, H., El Hamdaoui, A. et al., 2016. Chemical characterization and antifungal activities of four Thymus species essential oils against postharvest fungal pathogens of citrus. Industrial Crops and Products. 86: 95-101.

Ghellai, L., and Beral, L., 2015. Antibacterial efficacy of essential oil of Thymus capitatus, lactic acid and acetic acid against Escherichia coli in craw chicken meat, International Journal of Advanced Multidisciplinary Research (IJAMR). 2(6): 42–47.