Soil diazotrophic community structure altered in rice crop rotated with mungbean or maize in Cai Lay district, Tien Giang province

Do Thi Xuan * , Vo Thi Guong , Anna Rosling , Qiong Wang , Sadhna Alström and Nils Högberg

* Corresponding author (dtxuan@ctu.edu.vn)

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Received: 08 Sep 2016
Revised: 21 Nov 2016
Accepted: 31 Oct 2017
Published: 29 Nov 2017
DOI: 10.22144/ctu.jen.2017.042
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Do, T. X., Vo, T. G., Anna, R., Qiong, W., Sadhna, A., & Nils, H. (2017). Soil diazotrophic community structure altered in rice crop rotated with mungbean or maize in Cai Lay district, Tien Giang province. CTU Journal of Innovation and Sustainable Development , (07), 6-12. https://doi.org/10.22144/ctu.jen.2017.042
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No. 07 (2017)

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Abstract

Nitrogen availability is often one of the limiting factors for intensive rice (Oryza sativa) cultivation. Rotation of rice with upland crops partially reduces nutrient depletion. This study was carried out at CaiLay district, Tien Giang province with the aim to investigate the effect of alternating the rice crop with maize (Zea mays) and mungbean (Phaseolus aureus)in different rotational systems on the diazotrophic community in soilusing pyrosequencing of the nifH genes. The results showed that the community structure of the diazotroph communities in soils were different when rice crop was alternated with either maize or mungbean crop than that in rice monoculture. The nifH sequences were highly diverse and assigned to eleven bacterial phyla and one Archaeal phylum. Proteobacteria and Firmicutes were the most common phyla carrying the nifH gene. The propotion of the Archaea, Betaproteobacteria, Cyanobacteria, Deltaproteobacteria, Firmicutes, Gammaproteobacteria and Nitrospira was significantly different among the treatments. The relative abundances of Anaeromyxobacter, and Geobacter were greater in RRR than in the RMgR treatment while Heliobacterium and Desulfosporosinus were higher in the RMgR than those in the RRR and RMR treatment.
Keywords: bacterial community, diazotrophic, nifH gene, pyrosequencing, rice crop rotation

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