Development of an efficient in vitro plant regeneration protocol for indica rice varieties (Oryza sativa L.) in the Mekong Delta of Vietnam

Tran Thi Xuan Mai * , Tran Thi Cuc Hoa , Geert Angenon and Nguyen Thi Lien

* Corresponding author (ttxmai@ctu.edu.vn)

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Received: 13 Jun 2016
Revised: 09 Aug 2016
Accepted: 30 Mar 2017
Published: 31 Mar 2017
DOI: 10.22144/ctu.jen.2017.017
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Tran, T. X. M., Tran, T. C. H., Geert, A., & Nguyen, T. L. (2017). Development of an efficient in vitro plant regeneration protocol for indica rice varieties (Oryza sativa L.) in the Mekong Delta of Vietnam. CTU Journal of Innovation and Sustainable Development , (05), 141-149. https://doi.org/10.22144/ctu.jen.2017.017
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No. 05 (2017)

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Abstract

In this study, the effects of medium components and environmental conditions on callus induction and regeneration potential of two indica rice varieties (IR64 and MTL250) were evaluated.  Mature seeds were cultured on four different medium formulations including either Murashige and Skoog (MS) or Chu (N6) for callus induction. The MS medium was found more suitable than N6 medium in terms of callus induction frequency. The highest callus induction rate with good quality was obtained on MS medium containing 2mg/L 2,4D, 112mg/L B5 vitamin, 500mg/L proline,  500mg/L glutamine, 300mg/L casein hydrolysate, 30g/L sucrose and 4g/L phytagel. To investigate the best condition for callus growth, the influences of incubation temperatures and light conditions on callus induction of IR64 and MTL250 varieties were examined. The experimental results clearly showed that incubation temperatures at both 28oC and 32oC had no significant effects on callus induction in both varieties.  However, periodic illumination for 16 hours/day and complete darkness proved the best effect on callus induction in MTL250 and IR64, respectively. Among 07 different media used for shoot induction, the best results were obtained when calli of IR64 and MTL250 were cultured on medium containing MS including vitamins + 2.0 mg/L BAP + 0.5 mg/L NAA + 20g/L sucrose + 30g/L sorbitol + 6g/L phytagel. Shoot regeneration by using medium supplemented with kinetin, moreover, was very low or even failed in both varieties. This plant regeneration protocol is considered to be significant improved and promises to serve for breeding or genetic engineering of indica rice varieties in the Mekong Delta of Viet Nam.
Keywords: Callus induction, indica rice, MS medium, N6 medium, Shoot induction

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