Screening of aromatic rice lines by using molecular marker and sensory test

Nguyen Tri Yen Chi * and Truong Trong Ngon

* Corresponding author (ntychi@ctu.edu.vn)

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Received: 09 Dec 2015
Revised: 08 Aug 2016
Accepted: 30 Nov 2016
Published: 30 Nov 2016
DOI: 10.22144/ctu.jen.2016.049
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Nguyen, T. Y. C., & Truong, T. N. (2016). Screening of aromatic rice lines by using molecular marker and sensory test. CTU Journal of Innovation and Sustainable Development , (04), 100-104. https://doi.org/10.22144/ctu.jen.2016.049
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No. 04 (2016)

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Abstract

Aromatic rice has become popular owing to its aroma. Growing demand for aromatic rice has spurred interest of breeders. In this study, twenty five BC1F1 rice lines of backcross hybrid combinations were used to evaluate aromatic characteristics through sensory test and  using microsatellite markers. There are twelve lines had aroma and one no aroma were chosen though sensory test.  Allele Specific Amplification with four primers (External Antisense Primer, External Sense Primer, Internal Non-fragrant Sense  Primer, and Internal Fragrant Antisense Primer) were used for identifying fgr gene locus in 25 rice lines. Analysis of PCR results showed that BC1F1 population segregated into 1:1 ratio (homozygous fragrant: heterozygous non-fragrant). Thus, twelve promising lines were identified with homozygous fragrant genotype.
Keywords: Aromatic rice, microsatellite markers, sensory test

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References

Amarawathi, Y., Singh, R., Singh, A.K., Singh, V.P., Mohapatara, T., Sharma, T.R., 2008. Mapping of qualitative trait loci for basmati quality traits of rice (Oryza sativa L.) Mol. Breed. 21: 49-65.

Bergman, C.J., McClung, A.M., Pinson, S.R., Fjellstrom, R.G., 2002. Development of PCR-based markers associated with cooked rice kernel elongation and aroma. Proceedings of rice technical working group meeting, Arkansas. p. 53

Bounphanousay, C., Jaisil, P., Sanitchon, J., Fitzgerald, M., Hamilton, N.R.S., 2008. Chemical and molecular characterization of fragrance in black glutinous rice from Lao PDR. Asian J. of Plant Sci. 7: 1-7.

Bradbury, L.M.T., Fitzgerald, T.L., Henry, R.J., Jin, Q., Water, D.L.E., 2005a. The gene for fragrance genotyping in rice. Plant Biotech. J. 3: 363-370.

Bradbury, L.M.T., Fitzgerald, T.L., Henry, R.J., Jin, Q., Water, D.L.E., 2005b. A perfect marker for fragrance genotyping in rice. Mol. Breed. 16: 279- 283.

Cordeiro, G.M., Christopher, M.J., Henry, R.J., Reinke, R.F., 2002. Identification of microsatellite markers for fragrance in rice by analysis of the rice genome sequence. Mol. Breed. 9: 245-250.

Ghaffar, K., 2011. Marker aided selection for aroma in F2 populations of Rice African Journal of Biotechnology. 10(71): 15845-15848.

Itani, T., Tamaki, M., Hayata, T., Fushimi, K.H., 2004. Variation of 2-acetyl-1- pyrolline concentration in aromatic rice grains collected in the same region in Japan and factors affecting its concentration. Plant Prod. Sci. 7: 178-183.

Kovach, M.J., Calingacion, M.N., Fitzgerald, M.A., McCouch, S.R., 2009. The origin and evolution of fragrance in rice (Oryza sativa L.). Proc. Natl. Acad. Sci. USA. 106(34): 14444-14449.

Lorieux, M., Petrov, M., Huang, N., Guiderdoni, E., Ghesquiere, A., 1996. Aroma in rice: genetic analysis of quantitative trait. Theor. Appl. Genet. 93: 1145-1151.

Mohamad, O., Hadzim, K., Azlan, S., Abdullah, M.Z., Amiran, N., Nur Samahah, M.Z., 2008. Molecular screening for high quality rice. Retrieved November 20, 2011, from http://www.fao.org/ag/agp/agpc/doc/services/pbn/pbn195

Rogers, S.O., Bendich, A.J., 1988. Extraction of DNA from plant tissues. In: S. B. Gelvin, and R. A. Schilperoort (eds), Plant Molecular Biology Manual, A6, 1D10. Kluwer Acad. Publ., Dordrecht.

Sarhadi, W.A., Hien, N.L., Zanjani, M., Yosofzai, W., Yoshihashi, T., Hirata, Y., 2008. Comparative analyses for aroma and agronomic traits of native rice cultivars from Central Asia. J. Crop Sci. Biotech. 11 (1): 17-22.

Sood, B.C., Siddiq, E.A., 1978. A rapid technique for scent determination in rice. Indian J. Genet. Plant Breed. 38: 268–271.

Yi, M., Wea, K.T.N., Vanavichit, A., Chai-arree, W., Toojinda, T., 2009. Marker assisted backcross breeding to improve cooking quality traits in Myanmar rice cultivar Manawthukha. Field Crops Research. 113(2): 178–186.