Phung Tan Phat * and Le Hoang Ngoan

* Corresponding author (tanphat6194@gmail.com)

Main Article Content

Abstract

This paper introduces the extraction and purification of some polymethoxylated flavones from Ageratum conyzoides L., (Family-Asteraceae), growing in Can Tho city. Different extraction methods were used to study on the aerial part of the herb. From 1% HCl in water extracts, three polymethoxylated flavones have been isolated and identified. They were O‑methyl apigenin, sinensetin, and scutellareintetramethyl ether. Structures of isolated compounds were elucidated according to their 1H-NMR, 13C-NMR, HSQC, HMBC, MS spectra as well as referring to published article.
Keywords: polymethoxylated flavone, Ageratum conyzoides, O-methyl apigenin, sinensetin, scutellareintetramethyl ether

Article Details

References

Akao, Y., Ohguchi, K., Iinuma, M., Nozawa, Y., 2008. Interactive effects of polymethoxy flavones from Citrus on cell growth inhibition in human neuroblastoma SH-SY5Y cells. Bioorganic & Medicinal Chemistry. 16(6): 2803-2810.

Atindehou, M., Lagnika, L., Guérold, B., Strub, J.M., Zhao, M., Van Dorsselaer, A., Marchioni, E., Prévost, Gi., Haikel, Y., Taddéi, C., 2013. Isolation and identification of two antibacterial agents from Chromolaena odorata L. active against four diarrheal strains. Advances in Microbiology. 3: 115-121.

Dogra, N.K., 2015. A Review on traditional uses chemical constituents and pharmacology of ageratum conyzoides L. (Asteraceae). International Journal of Pharmaceutical & Biological Archive. 5(5): 33-45.

Gupta, V., 2010. New synthetic methods for biologically active aromatic heterocycles. Graduate Theses and Dissertations. Iowa State University. Ames, Iowa, United States.

Ho, P.H., 2000. An illustrated flora of Vietnam, part 3. 2nd edition. Youth Publishing House. Ho Chi Minh city. 1020 pages (in Vietnamese).

Huang, Y.S., Ho, S.C., 2010. Polymethoxy flavones are responsible for the anti-inflammatory activity of citrus fruit peel. Food Chemistry. 119(3): 868‑873.

Itoh, T., Ohguchi, K., Iinuma, M., Nozawa, Y., Akao, Y., 2008. Inhibitory effects of polymethoxy flavones isolated from Citrus reticulate on degranulation in rat basophilic leukemia RBL-2H3: enhanced inhibition by their combination. Bioorganic & medicinal chemistry. 16(16): 7592-7598.

Kim, H., Moon, J.Y., Mosaddik, A., Cho, S.K., 2010. Induction of apoptosis in human cervical carcinoma HeLa cells by polymethoxylated flavone-rich Citrus grandis Osbeck (Dangyuja) leaf extract. Food and Chemical Toxicology. 48(8): 2435-2442.

Lee, H., Kim, B., Kim, M., Ahn, J., 2015. Biosynthesis of two flavones, apigenin and genkwanin in Escherichia coli. Journal of Microbiology and Biotechnology. 25(9): 1442-1448.

Lee, S.H., Moon, B.H., Park, Y.H., Lee, E.J., Hong, S., Lim, Y.H., 2008. Methyl substitution effects on 1H and 13C NMR data of methoxyflavones. Bull. Korean Chem. Soc. 29(9): 1793-1796.

Loi, D.T., 2004. Vietnam's Herbal Plants and Remedies. 12th edition. Medicine Publishing House. Hanoi. 1274 pages (in Vietnamese).

Ming, L.C., 1999. Ageratum conyzoides: A tropical source of medicinal and agricultural products. Perspectives on new crops and new uses. 469-473.

Nasrin, F., 2013. Antioxidant and cytotoxic activities of Ageratum conyzoides stems. International Current Pharmaceutical Journal. 2(2): 33-37.

Nyemb, N., Adèle, M.D.B., Njikam, N., El, A., 2009. Antioxidant potential of aqueous leaf extract of Ageratum conyzoides Linn. in diabetic rats. Journal of Pharmacognosy and Phytotherapy. 1(4): 041-046.

Okunade, A.L., 2002. Ageratum conyzoides L.(Asteraceae). Fitoterapia. 73(1): 1‑16.

Pandith, H., Zhang, X., Thongpraditchote, S., Wongkrajang, Y., Gritsanapan, W., Baek, S.J., 2013. Effect of Siam weed extract and its bioactive component scutellarein tetramethyl ether on anti-inflammatory activity through NF-κB pathway. Journal of Ethnopharmacology. 147(2): 434-441.

Tominari, T., Hirata, M., Matsumoto, C., Inada, M., Miyaura, C., 2012. Polymethoxy flavonoids, nobiletin and tangeretin, prevent lipopolysaccharide-induced inflammatory bone loss in an experimental model for periodontitis. Journal of Pharmacological Sciences. 119(4): 390-394.

Xuan, T.D., Shinkichi, T., Hong, N.H., Khanh, T.D., Min, C.I., 2004. Assessment of phytotoxic action of Ageratum conyzoides L. (billy goat weed) on weeds. Crop protection. 23(10): 915-922.

Yam, M.F., Lim, V., Salman, I.M., Ameer, O.Z., Ang, L.F., Rosidah, N., Abdulkarim, M.F., Abdullah, G.Z., Basir, R., Sadikun, A., 2010. HPLC and anti-inflammatory studies of the flavonoid rich chloroform extract fraction of Orthosiphon stamineus leaves. Molecules. 15(6): 4452-4466.