Isolation and screening of carotenoid-producing Bacillus spp. from seashore saline soil and seawater at Hon Son islet
,
,
and
*
Article Sidebar
Full Text: 
PDF
Main Article Content
Abstract
Carotenoids are natural pigment compounds with a variety of colors. They are antioxidants and boost the immune system. It is interesting to examine strains of bacteria capable of producing carotenoids applied in the food industry, animal feed, and aquaculture. Rach Gia bay of Kien Giang province has a good ecosystem, and the carotenoid-producing microorganisms in these regions are very plentiful. However, there are fewer studies about them in this region. This study was conducted to isolate and select carotenoids-producing Bacillus from Hon Son islet of Rach Gia bay. Seashore saline soil and seawater samples were shocked at 65°C in 1 hour and plated on Difco sporulation medium. Colored colonies of bacteria were selected and incubated at 37°C on Difco sporulation medium. Pigments from these isolates were extracted in the mixture of methanol and chloroform (with the ratio 1:2 of v/v). The extracted solutions were scanned absorbance by spectrophotometer at the wavelength range from 400 to 600 nm. Bacterial isolates were identified by 16S rRNA sequencing. Twenty carotenoid-producing bacterial species were isolated, including five colony colors such as pale yellow, yellow, yellow-orange, pale pink, and pink. They were Gram-positive, positive - catalase test, motile, and endospore-forming. The results of extracted solutions from 20 bacterial species indicate that all the extracts have absorbance from 400-600 nm, this is the popular absorbance of carotenoids. Two high-carotenoid-producing species (HS3-7 and HS8-3) were analyzed the 16S rRNA gene sequence. They were showed high similarity with Bacillus vietnamensis species Marseille-P799 and Bacillus infantis species NRRL B-14911,...
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
References
Abdel-Fattah, A.M., Gamal-Eldeen, A.M., Helmy, W.A., & Esawy, M.A. (2012). Antitumor and antioxidant activities of levan and its derivative from the isolate Bacillus subtilis NRC1aza. Carbohydrate polymers, 89(2), 314-322. https://doi.org/10.1016/j.carbpol.2012.02.041.
Abdulkadir, M., & Waliyu, S. (2012). Screening and isolation of the soil bacteria for ability to produce antibiotics. European Journal of Applied Sciences, 4(5), 211-215. https://doi.org 10.5829/idosi.ejas.2012.4.5.2011
Amin, A., Khan, M.A., Ehsanullah, M., Haroon, U., Azam, S.M.F., & Hameed, A. (2012). Production of peptide antibiotics by Bacillus sp: GU 057 indigenously isolated from saline soil. Brazilian Journal of Microbiology, 43(4): 1340-1346. http://doi.org/10.1590/S1517-83822012000400015
Asker, D., Awad, T. S., Beppu, T., & Ueda, K. (2012). Isolation, characterization, and diversity of novel radio tolerant carotenoid-producing bacteria. In J. L. Barredo (Ed.). Microbial Carotenoids from Bacteria and Microalgae (Methods and Protocols) (pp.21-60). Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-879-5_3
Britton, G., Liaaen-Jensen, S., & Pfander, H. (2004). Section II: Main List Natural carotenoids. In G. Britton & H. Pfander (Eds.). Carotenoids: Handbook, First Edition (pp.29-296). Birkhäuser Basel. Basel.
Butnariu, M. (2016). Methods of analysis (extraction, separation, identification, and quantification) of carotenoids from natural products. Journal of Ecosystem & Ecography, 6(2): 1-19. https://doi.org/10.4172/2157-7625.1000193
Duc, L. H., Fraser, P. D., Tam, N. K., & Cutting, S. M. (2006). Carotenoids present in halotolerant Bacillus spore formers. FEMS microbiology letters, 255(2), 215-224. https://doi.org/10.1111/j.1574-6968.2005.00091.x
Felsenstein, J. (1985). Confidence limits on phylogenies: an approach using the bootstrap. evolution, 39(4),783-791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
Fiedor, J., & Burda, K. (2014). Potential role of carotenoids as antioxidants in human health and disease. Nutrients, 6(2), 466-488. https://doi.org/10.3390/nu6020466.
Holt, J. G., Kreig, P. H. A., & Sneath, J. T. S. (1994). Bergey’s Manual of Determinative Bacteriology (9th ed.). Williams and Wilkins, Baltimore.
Indra Arulselvi, P., Umamaheswari, S., Ranandkumar, S. G., Karthik, C., & Jayakrishna, C. (2014). Screening of yellow pigment producing bacterial isolates from various eco-climatic areas and analysis of the carotenoid produced by the isolate. Journal of Food Processing & Technology, 5(1). https://doi.org/10.4172/2157-7110.1000292
Khaneja, R., Perez‐Fons, L., Fakhry, S., Baccigalupi, L., Steiger, S., To, E., Sandmann, G., Dong , T.C., Ricca, E., & Cutting, S. M. (2010). Carotenoids found in Bacillus. Journal of applied microbiology, 108(6), 1889-1902. https://doi.org/10.1111/j.1365-2672.2009.04590.x
Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular biology and evolution, 35(6), 1547-1549. https://doi.org/10.1093/molbev/msy096
Liaaen-Jensen, S., & Jensen, A. (1971). Quantitative determination of carotenoids in photosynthetic tissues. In Methods in enzymology (Vol. 23, pp. 586-602). Academic Press. https://doi.org/10.1016/S0076-6879(71)23132-3
Mitchell, C., Iyer, S., Skomurski, J. F., & Vary, J. C. (1986). Red pigment in Bacillus megaterium spores. Applied and environmental microbiology, 52(1), 64-67.
Nicholson, W. L., Munakata, N., Horneck, G., Melosh, H. J., & Setlow, P. (2000). Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments. Microbiology and molecular biology reviews, 64(3), 548-572. https://doi.org/10.1128/MMBR.64.3.548-572.2000
Nielsen, P., Fritze, D., & Priest, F. G. (1995). Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology, 141(7), 1745-1761. https://doi.org/10.1099/13500872-141-7-1745
Nugraheni, S. A., Khoeri, M. M., Kusmita, L., Widyastuti, Y., & Radjasa, O. K. (2010). Characterization of carotenoid pigments from bacterial symbionts of seagrass Thalassia hemprichii. Journal of Coastal Development, 14(1), 51-60.
Oktari, A., Supriatin, Y., Kamal, M., & Syafrullah, H. (2017). The bacterial endospore stain on schaeffer fulton using variation of methylene blue solution. Journal of Physics: Conference Series (Vol. 812, No. 1, p. 012066). IOP Publishing.
Dang, H., & Lovell, C. R. (2000). Bacterial primary colonization and early succession on surfaces in marine waters as determined by amplified rRNA gene restriction analysis and sequence analysis of 16S rRNA genes. Applied and environmental microbiology, 66(2), 467-475. https://doi.org/10.1128/AEM.66.2.467-475.2000
Andrews, P. H., & Sokal, R. R. (1973). Numerical taxonomy: The principles and practice of numerical classification. WH Freeman.
Tamura K., Nei M., and Kumar S. (2004). Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences (USA), 101, 11030-11035. https://doi.org/doi:10.1073/pnas.0404206101
Tamura, K., & Kumar, S. (2002). Evolutionary distance estimation under heterogeneous substitution pattern among lineages. Molecular biology and evolution, 19(10), 1727-1736. https://doi.org/10.1093/oxfordjournals.molbev.a003995
Tittsler, R. P., & Sandholzer, L. A. (1936). The use of semi-solid agar for the detection of bacterial motility. Journal of bacteriology, 31(6), 575.
Vílchez, C., Forján, E., Cuaresma, M., Bédmar, F., Garbayo, I., & Vega, J. M. (2011). Marine carotenoids: biological functions and commercial applications. Marine Drugs, 9(3), 319-333. https://doi.org/10.3390/md9030319
Yoon, J. H., Kim, I. G., Kang, K. H., Oh, T. K., & Park, Y. H. (2003). Bacillus marisflavi sp. nov. and Bacillus aquimaris sp. nov., isolated from sea water of a tidal flat of the Yellow Sea in Korea. Journal of Medical Microbiology, 53(5), 1297-1303. https://doi.org/10.1099/ijs.0.02365-0.