Khang Luu Tang Phuc * , Tran Thi Phuong Dung and Nguyen Huu Giau

* Correspondence: Khang Luu Tang Phuc (email:

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The present study attempted to understand the histopathological index of striped catfish (Pangasianodon hypophthalmus) in challenge with Edwardsiella ictaluri, the causative agent of bacillary necrosis of Pangasius. Total of 355 healthy striped catfish juveniles was challenged with E. ictaluri (25 control; 330 infected). After the challenge, a total of 355 samples of the trunk kidney, liver, and spleen were collected at five different time points including just prior to infection, 24, 48, 264 and 312 hours post-infection (hpi) to measure the represented tissue damage of the fish. Results showed that many areas of the diseased liver, kidney, and fish organ tissues have been congested and hemorrhaged early at 24 hpi. The number of macrophage centers in the kidney and spleen increased at 24 - 48 hpi. Tissue damage (multifocal hemorrhages; necrosis) increased sharply from the period 48 - 256 hpi. The increased number of dead fish throughout infection, particularly the period from 48 to 200 hpi. At 312 hpi, the histopathological index was the highest, so the mortality rate was the highest during the experimental period.

Keywords: Edwardsiella ictaluri, disease resistance, histopathology characteristics, Pangasianodon hypophthalmus

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Areechon, N., & Plumb, J. A. (1983). Pathogenesis of Edwardsiella ictaluri in channel catfish, Ictalurus punctatus. Journal of the World Mariculture Society, 14(1‐4), 249-260.

Baldwin, T. J., & Newton, J. C. (1993). Pathogenesis of enteric septicemia of channel catfish, caused by Edwardsiella ictaluri: Bacteriologic and light and electron microscopic findings. Journal of Aquatic Animal Health, 5(3), 189–198.

Camp, K. L., Wolters, W. R. & Rice, C. D. (2000). Survivability and immune responses after challenge with Edwardsiella ictaluri in susceptible and resistant families of channel catfish, Ictalurus punctatus. Fish Shellfish Immunology, 10(6), 475-487.

Cercadillo-Ibarguren, I., España Tost, A. J., Arnabat Domínguez, J., Valmaseda Castellón, E., Berini Aytés, L., & Gay Escoda, C. (2010). Histologic evaluation of thermal damage produced on soft tissues by CO2, Er, Cr: YSGG and diode lasers. Medicina Oral, Patología Oral y Cirugia Bucal, 15(6), 912-918.

Coolidge, B. J, & Howard, R. M. (1979). Animal histology procedures of the pathological technology section of the National Cancer Institute, 2nd edition. National Institutes of Health, Bethesda. 209 pages.

Crumlish, M., Pham, T. C., Koesling, J., Vu, T., V, & Gravningen, K. (2010). Experimental challenge studies in Vietnamese catfish, Pangasianodon hypophthalmus (Sauvage), exposed to Edwardsiella ictaluri and Aeromonas hydrophila. Journal of fish diseases, 33(9), 717-722.

Crumlish, M., Tu, D. T., Turnbull, J. F., Nguyen, N. T. N., & Ferguson, H. W. (2002). Identification of Edwardsiella ictaluri from diseased freshwater catfish, Pangasius hypophthalmus (Sauvage), cultured in the Mekong Delta, Vietnam. Journal of Fish Diseases, 25(12), 733-736.

De Silva, S. S., & Nguyen, P. T. (2011). Striped catfish farming in the Mekong Delta, Vietnam: a tumultuous path to a global success. Reviews in Aquaculture, 3(2), 45-73.

De Silva, S. S., Ingram, B. A., Nguyen, P. T., Bui, T. M., Gooley, G. J., & Turchini, G. M. (2010). Estimation of nitrogen and phosphorus in effluent from the striped catfish farming sector in the Mekong Delta, Vietnam. Ambio, 39(7), 504-514.

Dhar, A. K., Manna, S. K., & Thomas Allnutt, F. C. (2014). Viral vaccines for farmed finfish. Virusdisease, 25(1), 1-17.

Dyková, I., Žák, J., Blažek, R., Reichard, M., Součková, K., & Slabý, O. (2022). Histology of major organ systems of Nothobranchius fishes: short-lived model species. Journal of Vertebrate Biology, 71(21074), 1-50.

Fanta, E., Rios, F. S. A., Romão, S., Vianna, A. C. C., & Freiberger, S. (2003). Histopathology of the fish Corydoras paleatus contaminated with sublethal levels of organophosphorus in water and food. Ecotoxicology and environmental safety, 54(2), 119-130.

Ferguson, H. W. (1989). Systemic pathology of fish. A text and atlas of comparative tissue responses in diseases of teleosts. Iowa State University Press.

Ferguson, H. W., & McCarthy, D. H. (1978). Histopathology of furunculosis in brown trout Salmo trutta L. Journal of Fish Diseases, 1(2), 165-174.

Ferguson, H. W., Turnbull, J. F., Shinn, A., Thompson, K., Tu, D. T., & Crumlish, M. (2001). Bacillary necrosis in farmed Pangasius hypophthalmus (Sauvage) from the Mekong Delta, Vietnam. Journal of Fish Diseases, 24(9), 509-513.

Gernhöfer, M., Pawert, M., Schramm, M., Müller, E., & Triebskorn, R. (2001). Ultrastructural biomarkers as tools to characterize the health status of fish in contaminated streams. Journal of Aquatic Ecosystem Stress and Recovery, 8(3), 241-260.

Grizzle, J. M., & Rogers, W. A. (1976). Anatomy and histology of the channel catfish. Auburn, Ala. : Auburn University, Agricultural Experiment Station, 100 pages.

Harder, W. 1975. Anatomy of fishes. E. Schweizerbart'sche Verlagsbuchhandlung (Nagele v. Obermiller). Stuttgart, West Germany, 744 pages.

Hawke, J. P., Kent, M., Rogge, M., Baumgartner, W., Wiles, J., Shelley, J., L., Savolainen, C., Wagner, R., Murray, K., & Peterson, T. S. (2013). Edwardsiellosis caused by Edwardsiella ictaluri in laboratory populations of zebrafish Danio rerio. Journal of aquatic animal health, 25(3), 171-183.

Hinton, D. E., & Lauren, D. J. (1990) Liver structural alterations accompanying chronic toxicity in fishes: potential biomarkers of exposure. In: Biomarkers of Environmental Contamination, edited by J.F. McCarthy and L.R. Shugart Lewis Publishers, Boca Raton, FL, pp. 17-57.

Hinton, D. E., Snipes, R. L., & Kendall, M. W. (1972). Morphology and enzyme histochemistry in the liver of largemouth bass (Micropterus salmoides). Journal of the Fisheries Board of Canada, 29(5), 531-534.

Huizinga, H. W., Esch, G. W., & Hazen, T. C. (1979). Histopathology of red‐sore disease (Aeromonas hydrophila) in naturally and experimentally infected largemouth bass Micropterus salmoides (Lacepede). Journal of Fish Diseases, 2(4), 263-277.

Iwanowicz, L. R., Griffin, A. R., Cartwright, D. D., & Blazer, V. S. (2006). Mortality and pathology in brown bullheads Amieurus nebulosus associated with a spontaneous Edwardsiella ictaluri outbreak under tank culture conditions. Diseases of Aquatic Organisms, 70(3), 219–225.

Jarboe, H., Bowser, P., & Robinette, H. (1984). Pathology associated with a natural Edwardsiella ictaluri infection in channel catfish (Ictalurus punctatus Rafinesque). Journal of Wildlife Diseases, 20(4), 352–354.

Kibenge, F. S., & Strange, R. J. (2021). Introduction to the anatomy and physiology of the major aquatic animal species in aquaculture. In Aquaculture Pharmacology (pp. 1-111). Academic Press.

Klontz, G. W. (1966). Bacterial diseases of the Salmonidae in the Western United States: pathogenesis of furunculosis in rainbow trout. American Journal of Veterinary Research, 27, 1455-1460.

Le, H. P. (2013). Summary report on scientific and technological results of the project "Improving the efficiency of using inactivated vaccines through protein heat shock". Research Institute For Aquaculture No.2, 165 pages.

Liu, C., Ma, J., Zhang, D., Li, W., Jiang, B., Qin, Z., ... & Wang, Q. (2021). Immune Response and Apoptosis-Related Pathways Induced by Aeromonas schubertii Infection of Hybrid Snakehead (Channa maculata ♀× Channa argus ♂). Pathogens, 10(8), 997.

Loch, T. P., Hawke, J. P., Reichley, S. R., Faisal, M., Del Piero, F., & Griffin, M. J. (2017). Outbreaks of edwardsiellosis caused by Edwardsiella piscicida and Edwardsiella tarda in farmed barramundi (Lates calcarifer). Aquaculture, 481, 202-210.

Mahdy, O. A., Abdelsalam, M., Abdel‐Maogood, S. Z., Shaalan, M., & Salem, M. A. (2022). First genetic confirmation of Clinostomidae metacercariae infection in Oreochromis niloticus in Egypt. Aquaculture Research, 53(1), 199-207.

Mamun, A. A., Nasren, S., Rathore, S. S., & Mahbub Alam, M. M. (2022). Histopathological Analysis of Striped Catfish, Pangasianodon hypophthalmus (Sauvage, 1878) Spontaneously Infected with Aeromonas hydrophila. Jordan Journal of Biological Sciences, 15(1), 93 – 100.

Mawdesley-Thomas, L. E. (1971). Neoplasia in fish: a review. Current topics in comparative pathobiology, 1, 87-170.

Newton, J. C., Wolfe, L. G., Grizzle, J. M., & Plumb, J. A. (1989). Pathology of experimental enteric septicaemia in channel catfish, Ictalurus punctatus (Rafinesque), following immersion-exposure to Edwardsiella ictaluri. Journal of Fish Diseases, 12(4), 335–347.

Nguyen, N. P. (2014). Environmental factors affecting the pathogenesis of Edwardsiella ictaluri in striped catfish Pangasianodon hypophthalmus (Sauvage) (master’s thesis). University of Stirling.

Nguyen, P. T., & Do, O. T. H. (2010). Striped catfish aquaculture in Vietnam: a decade of unprecedented development. In Success stories in Asian aquaculture (131-147). Springer, Dordrecht.

Nguyen, V. T., Tran, P. H., Kim, O. T. P., Nguyen, S. V., Trinh, T. T. & Nguyen, N. H. (2022). Accuracies of genomic predictions for disease resistance of striped catfish to Edwardsiella ictaluri using artificial intelligence algorithms. G3, 12(1), 1 - 13.

Patt, D. I., & Patt, G. R. (1969). Comparative vertebrate histology. Harper and Row, New York, New York, USA.

Petrie-Hanson, L., Romano, C. L., Mackey, R. B., Khosravi, P., Hohn, C. M., & Boyle, C. R. (2007). Evaluation of zebrafish Danio rerio as a model for enteric septicemia of catfish (ESC). Journal of Aquatic Animal Health, 19(3), 151-158.

Quach, T. V. C. (2017). The current status antimicrobial resistance in Edwardsiella ictaluri and Aeromonas hydrophila cause disease on the striped catfish farmed in the Mekong Delta (Doctoral thesis). Can Tho University.

Sakai, T., Yuasa, K., Ozaki, A., Sano, M., Okuda, R., Nakai, T., & Iida, T. (2009). Genotyping of Edwardsiella ictaluri isolates in Japan using amplified‐fragment length polymorphism analysis. Letters in applied microbiology, 49(4), 443-449.

Santander, J., Kilbourne, J., Park, J. Y., Martin, T., Loh, A., Diaz, I., Rojas , R., Segovia, C., DeNardo, D., & Curtiss III, R. (2014). Inflammatory effects of Edwardsiella ictaluri lipopolysaccharide modifications in catfish gut. Infection and immunity, 82(8), 3394-3404.

Shah, K. K., Pritt, B. S., & Alexander, M. P. (2017). Histopathologic review of granulomatous inflammation. Journal of clinical tuberculosis and other Mycobacterial Diseases, 7, 1-12.

Shotts, E. B., Gaines, J. L., Martin, L., & Prestwood, A. K. (1972). Aeromonas-induced deaths among fish and reptiles in an eutrophic inland lake. Journal of the American Veterinary Medical Association, 161(6), 603-607.

Soto, E., Illanes, O., Revan, F., Griffin, M., & Riofrio, A. (2013). Bacterial distribution and tissue targets following experimental Edwardsiella ictaluri infection in Nile tilapia Oreochromis niloticus. Diseases of Aquatic Organisms, 104(2), 105-112.

Suanyuk, N., Rogge, M., Thune, R., Watthanaphiromsakul, M., Champhat, N., & Wiangkum, W. (2013). Mortality and pathology of hybrid catfish, Clarias macrocephalus (Günther) × Clarias gariepinus (Burchell), associated with Edwardsiella ictaluri infection in southern Thailand. Journal of Fish Diseases, 37, 385–395.

Subagja, J., Slembrouck, J., & Legendre, M. (1999). Larval rearing of an Asian catfish Pangasius hypophthalmus (Siluroidei, Pangasiidae): Analysis of precocious mortality and proposition of appropriate treatments. Aquatic Living Resources, 12(1), 37-44.

Thophon, S., Kruatrachue, M., Upatham, E. S., Pokethitiyook, P., Sahaphong, S., & Jaritkhuan, S. (2003). Histopathological alterations of white seabass, Lates calcarifer, in acute and subchronic cadmium exposure. Environmental pollution, 121(3), 307-320.

VASEP. (2022). Striped catfish (Pangasianodon hypophthalmus) exports in Vietnam in 2021 with great efforts.

VASEP. (2022). Vietnam fisheries production in the first 6 months of the year reached nearly 4.2 million tons.

Wolke, R. E. (1975). Pathology of bacterial and fungal diseases affecting fish (pp. 33-116). Madison, Wisconsin: University of Wisconsin Press.

Ye, S., Li, H., Qiao, G., & Li, Z. (2009). First case of Edwardsiella ictaluri infection in China farmed yellow catfish Pelteobagrus fulvidraco. Aquaculture, 292, 6-10.