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Rosa hybrida L. var. Lửa has been one of the most important roses for a long time and is most often used for ornamental purposes in Sadec city, Dong Thap province. The study aimed to induce variation through in-vitro gamma-ray irradiation in the rose variety ‘Lửa’ for further improvement in rose breeding. In-vitro single node cuttings (25 in-vitro cuttings per treatment with four replications) were irradiated with different doses of γ-rays (0, 5, 10, 15, 20, 25, or 30 Gy) using a 60Co source (India) at Da Lat Nuclear Research Institute. The γ-irradiated explants were then cultured aseptically on Murashige and Skoog’s basal medium supplemented with 1.0 mg/L BAP to induce multiple shoots, shoot proliferation, and acclimatization at Division of Vegetables, Flowers and Landscaping of Southern Horticultural Research Institute (SOFRI). The LD50 dose was determined at 20 - 25 Gy treatments and these doses affected the multiplication rate, growth in-vitro, and ex-vitro survival rate. Three types of mutants with altered or novel flower color in comparison to the original flower color were isolated, such as Type 1 with orange-pink (Red 52C), Type 2 with pink (Red 54B), and Type 3 with Red 45AB with Red 54B bicolor.
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Aamir, S. S., Baig, M. M. Q., Ahmad, T., Ghafoor, A., Hafiz, I. A., Abbasi, N. A., ... & Yaseen, M. (2016). Molecular and morphological characterization of rose mutants produced via in vitro mutagenesis. Philipp. Agr. Sci, 99, 25-33.
Arnold, N. P., Barthakur, N. N., & Tanguay, M. (1998). Mutagenic effects of acute gamma irradiation on miniature roses: target theory approach. HortScience, 33(1), 127-129.
Baig, M. M. Q., Hafiz, I. A., Abbasi, N. A., Yaseen, M., Akram, Z., & Donnelly, D. J. (2012). Reduced-stature Rosa species through in vitro mutagenesis. Canadian Journal of Plant Science, 92(6), 1049-1055.
Bala, M., & Singh, K. P. (2015). In vitro mutagenesis in rose (Rosa hybrida L.) cv. Raktima for novel traits. Indian Journal of Biotechnology, Vol 14, October 2015, pp 525-531.
Bala, M., & Singh, K. P. (2016). Gamma irradiation of in-vitro proliferated cultures of rose (Rosa hybrida) for induction of novel mutants. Indian Journal of Agricultural Sciences, 86(1), 137-142.
Chakrabarty, D., & Datta, S. K. (2010). Application of RAPD markers for characterization of γ-ray-induced rose mutants and assessment of genetic diversity. Plant Biotechnology Reports, 4(3), 237-242.
Datta, S. K. (1997). Mutation studies on garden roses: a review. Proc. Ind. Nat. Sci. Acad B, 63, 107-126.
Dao T B, Nguyen P D, Do Q M, Vu T H, Le T L, Nguyen H D and Nguyen X L. (2006). In vitro mutagenesis of chrysanthemum for breeding. Plant Mutation Reports 1: 26–7
Ibrahim, R. (1999). In vitro mutagenesis in roses. PhD. Thesis in Applied Biological Sciences-Cell and Gene Biotechnology, University Gent, Belgium.
International Atomic Energy Agency. (2021). IAEA Mutant Variety Database. International Atomic Energy Agency, Vienna. 20 June 2020. https://mvd.iaea.org/
Jain, S. M. (2006). Mutation-assisted breeding for improving ornamental plants. In XXII International Eucarpia Symposium, Section Ornamentals, on Breeding for Beauty. Acta Hortic. 714, 85-98.
Kahrizi, Z. A., Kermani, M. J., Amiri, M. E. and Vedadi, S. (2011). Identifying the correct dose of gamma-rays for in vitro mutation of rose cultivars. Acta Hortic. 923, 121-127.
Kim, G., Koh, C., Gi, G., Choi, K., & Song, H. (2006). In vitro mutant induction by irradiation of gamma-ray in Rosa hybrida Hort. Korean Journal of Horticultural Science & Technology, 24(4), 497-502.
Thanh, L. N. L. (2020). Study on rapid propagation of Fire rose (Rosa hybrida L.) by tissue culture method. Journal of Agriculture and Rural Development, No. 1 p. 65-70.
Murashige, T., & Skoog, F. (1962). A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiol Plant 15(3): 473-497.
Nonomura, T., Ikegami, Y., Morikawa, Y., Matsuda, Y., & Totoda, H. (2001). Induction of morphologically changed petals from mutagen-treated apical buds of rose and plant regeneration from varied petal-derived calli. Plant Biotechnology 18: 233–6.
Singh K P, Singh B, Raghava S P S and Kalia C S. (2000). Induced flower colour mutations in carnation through in vitro application of chemical mutagen, Indian Journal of Genetics: 60 535-9.
Smilansky, Z., Umiel, N. & Zieslin, N. (1986). Mutagenesis in roses (cv. Mercedes). Environmental and experimental botany, 26(3), 279-283.
Yamaguchi H, Shimizu A, Degi K and Morishita T. 2008. Effects of dose and dose rate of gamma-ray irradiation on mutation induction and nuclear DNA content in chrysanthemum. Breeding Science 58: 331–5.