Nguyen Ngoc Tan * and Cao Ngoc Diep

* Corresponding author (

Main Article Content


Abstract. High lipid (fats and oils) concentration contained in wastewater from restaurants and canteens released into environment directly, leading to be pollutant water and logging drainpipe systems. The lipid-degradation capability of lipid-degrading yeast was investigated for possible application in treatment of lipids-contaminated wastewater. Twenty-eight yeast isolates were isolated from 11 lipid-contaminated wastewater samples from many restaurants and canteens in Ninh Kieu district of Can Tho city, Vietnam. There were 15 isolates produced halo zones on Tw20 agar medium and  determined to have ability of lipid-degradation, three of which (B1, ST, Da2a isolates) were found to have the high ability of lipid-degradation by measuring development of halo zone diameter during 72 hours and identified by ITS-PCR technique and DNA sequencing. After 7 days of cultivation, the rate of the degradation of lipid contaminated in wastewater by strain B1, ST, Da2a were 74.14%, 83.03% and 80.7%, respectively. The results of DNA sequencing were compared with GenBank database of NCBI by BLAST N software. The sequences from selected isolates showed high degrees of similarity to those of the GenBank references (between 97% and 99%). Isolates of B1 and ST were 99% of similarity with Candida palmioleophila and Meyerozyma quilliermondii, respectively. Da2a isolate was 97% of similarity with Candida tropicalis.Keywords: Candida, canteens and restaurants, lipid-contanminated wastewater, lipid-degradation yeast, lipid degradion, vegetable oil
Keywords: Candida, canteens and restaurants, lipid-contaminated wastewater, lipid-degradation yeast, lipid degradation, vegetation oil

Article Details


Akiyama, S., 1991. Degradation of fat and oil by “Bacillus subtilis BN 1001”. Success in keeping high concentration of BN Clean. Yushi, Present situation and prospect of recovered oil use, J-GLOPAL. 44: 46-51.

Bataiche, I., Kacem-chaouche, N., Youcef-ali, M., Kara-ali, M., Dehimat, L., and Thonart, P., 2014. Isolation and characterization of highest degrading – yeast Yarrowia lipolytica and its impact minimal only environment. International Journal of Recent Scientific Research. 5(1): 228-232.

Bednarski, W., Adamezak, M., Kowalewska-Piontas, J., Zadenowski, R. 1994. Biotechnological methods for the up-grading and modification of animal waste fats. Acta Biotechnol. 14(4):387-393.

Diep, C.N., Phong, N.T., and Duyen, N.T., 2014. Isolation and characterization of lipid-degrading bacteria in wastewater of food processing plants and restaurants in Can Tho city, Vietnam. American Journal of Life Sciences. 2(6): 382-388.

General statistics office of Vietnam. 2014. Statistical Handbook of Vietnam 2014. Available from

Hasanuzzaman, M., Umadhay-Briones, K. M., Zsiros, S. M., Morita, N., Nodasaka, Y., Yumoto, I., and Okuyama, H., 2004. Isolation, identification, and characterization of a novel, oil-degrading bacterium, Pseudomonas aeruginosa T1. Curr. Microbiol. 49(2): 108-114.

Kurtzman, C.P., Fell, J.W., Teun, B., and Vincent, R., 2010. Methods for Isolation, Phenotypic Characterization and Maintenance of Yeasts. The Yeasts Book, pp. 87-110.

Matsumiya, Y., Wakita, D., Kimura, A., Sanpa, S., and Kupo, M., 2007. Isolation and characterization of a lipid-degrading bacterium and its application to lipid-containing wastewater treatment. J. Biosci. Bioeng. 103(4): 325-330.

Okuda, S., Ito, K., Ozawa, H., and Izaki, K., 1991. Treatment of lipid containing wastewater using bacteria which assimilate. J. Ferment. Bioeng. 71: 424-429.

Rogers, S.O. and Bendich. A.J., 1989. Extraction of DNA from plant tissues. Plant Molecular Biology Manual, A6. 1-10.

Saxena R.K., Sheoran A., Giri B., and Davidson, S., 2003. Purification strategies for microbial lipases. J Microbiol Methods. 52:1-18.

Surimogi, D., 2009. Edible oil degradation by using yeast coculture of Rhodotorula pacifica ST3411 and Cryptococcus laurentii ST3412. Appl. Microbiol. Biotechnol. 82: 351-357.

Suzuki, T., Nakayama, T., Kurihara, T., Nishimo, T., and Esuki, N., 2001. Cold-active lipolytic activity of pysochrotrophic Acinetobacter sp. strain No 6. J. Biosci. Bioeng. 92: 144-148.

Wakelin, N.M. and Forster, C.F., 1997. An investigation into microbial removal of fats, oils and greaves. Biores Technol. 59: 37-43.