Vo Thi Bich Thuy * , Huynh Ky , Tran Thi Ba , Swee Keong Yeap and Nguyen Loc Hien

* Correspondence: Vo Thi Bich Thuy (email: vtbthuy@ctu.edu.vn)

Main Article Content

Abstract

ABSTRACTThe level of polymorphism in chili was studied using ISSR marker. Polymorphism of sixteen chili varieties was evaluated with 15 ISSR primers. These 15 ISSR primers have generated 136 DNA bands, 102 being polymorphic, with an average of 9.06 bands per primer. The evaluation of the dendrogram obtained by UPGMA allowed the differentiation of all the varieties. Four varieties were separated in distinct group in which showed complete correspondence to its observed characters. DNA profile of 16 chili varieties based on ISSR marker revealed the potential of digital fingerprints of all varieties examined. 
Keywords: Polymorphism, chili, ISSR marker, genetic diversity

Article Details

References

Adetula, O.A., 2006. Genetic diversity of Capsicum using random amplified polymorphic DNAs. Afr. J. Biotech. 5: 120–122.

Akbar, N., Ahmad, H., Ghafoor, S., Begum, K., Gul, S., Afridi, I., Muhammad, I., Khan, I.A., 2010. Estimation of genetic diversity in Capsicum germplasm using randomly amplified polymorphic DNA. Asian J. Agric. Sci. 2(2): 53-56.

Bardakci, F., 2001. Random amplified polymorphic DNA (RAPD) markers, Turk. J. Biol. 25: 185-196.

Bosland, P.W., Baral, J.B., 2007. ‘Bhut Jolokia’- the world's hottest known chile pepper is a putative naturally occurring interspecific hybrid. HortScience. 42(2): 222-224.

Bosland, P.W., 1996. Capsicums: innovative uses of an ancient crop, J. Janick (Ed.), Progress in New Crops, ASHS Press, Arlington, VA), pp. 479-487

De Mejia, G.E., Quintanar-Hernandez, A., Loarca-Pina, G., 1998. Antimutagenic activity of carotenoids in green peppers against some nitroarenes. Mutat. Res. 416(1–2): 11–19.

Ilbi, H., 2003. RAPD markers assisted varietal identification and genetic purity test in pepper, Capsicum annuum. Scientia Hortic. 97: 211-218.

Kumar, L.D., Kathirvel, M., Rao, G.V., Nagaraju, J., 2001. DNA profiling of disputed chilli samples (Capsicum annuum) using ISSR–PCR and FISSR–PCR marker assays. Forensic Sci. Int. 116: 63-68.

Maoka, T., Mochida, K., Ito, Y., Fujiwara, Y., Hashimoto, K., Enjo, F., Ojata, M., Nobukumi, Y., Tokuda, H., Nishino, H., 2001. Cancer chemopreventive activity of carotenoids in the fruits of red paprika Capsicum annuum L., Cancer Lett. 172(2): 103-109.

Moonmoon, S., 2006. Random amplified polymorphic DNA markers (RAPD) for genetic variation study among tomato varieties. M.S. Thesis. Dept. of Biotechnology. Bangladesh Agricultural University, Mymensingh.

Morre, D.M., Morre ,D.J., 2006. Catechin-vanilloid synergies with potential clinical applications in cancer, Rejuven. Res. 9(1): 45–55.

Paran, I., Aftergoot, E., Shifriss, C., 1998. Variation in Capsicum annuum revealed by RAPD and AFLP markers. Euphytica. 99(3): 167-173.

Patel, A.S., Sasidharan, N., Ashish, G.V., Vinay, K., 2011. Genetic relation in Capsicum annuum [L.] cultivars through microsatellite markers: SSR and ISSR. Electr. J. Plant Breed. 2: 67-76.

Pickersgill, B., 1998. The genus Capsicum: a multidisciplinary approach to the taxonomy of cultivated and wild plants. Biol. Zent. 107: 381–389.

Rodriguez, J.M., Berke, T., Engle, L., Nienhius, J., 1999. Variation among Capsicum species revealed by RAPD markers. Theor. Appl. Genet. 99: 147–156.

Sitthiwong, K., Matsui, T., Sukprakarn, S., 2005. Classification of pepper (Capsicum annuum L.) accessions by RAPD analysis, Biotechnology. 4(4): 305–309.

Toquica, S.P., Rodríguez, F., Martínez, E., Duque, M.C., Tohme, J., 2003. Molecular characterization by AFLPs of Capsicum Germplasm from the Amazon Department in Colombia. Genet. Resour. Crop Evol. 50: 639-647.

Walsh, B.W., Hoot, S.B., 2001. Phylogenetic relationships of Capsicum (Solanaceae) using DNA sequences from two noncoding regions: the chloroplast atpB-rbcL spacer region and nuclear waxy introns. Int. J. Plant Sci. 162(6): 1409-1418.

Wang, J.J., Fan, M.J., 1998. Comparison on microsateliite DNA and RAPD markers for germplasm identification of Capsicum annuum L. J. Agric. Res. China. 47: 267-282.

Williams, J.G., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey, S.V., 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers, Nucleic Acids Res. 18: 6531–6535.

Yang, R., Kong, J., Wu, X., Deng, Z., Chen, Q., Liu, W., 2005. Application of ISSR markers in genetic polymorphism of Capsicum frutescens L. Journal of Shanghai University (Natural Science Edition). pp. 4-20.

Most read articles by the same author(s)