Study on the optimal conditions for fucoidan extraction from Kappaphycus alvarezii

Hoang Thi Ngoc Nhon * , Dinh Thi Huyen and Nguyen Van Nguyen Thinh

* Corresponding author (hoangthingocnhon1002@gmail.com)

Article Sidebar

Full Text: PDF

Received: 14 Jan 2019
Revised: 06 Jun 2019
Accepted: 30 Aug 2019
Published: 31 Jul 2019
DOI: 10.22144/ctu.jen.2019.020
Views
333
Downloads
159
Crossref
Scopus
Google Scholar
Europe PMC
How to Cite
Hoang, T. N. N., Dinh, T. H., & Nguyen, V. N. T. (2019). Study on the optimal conditions for fucoidan extraction from Kappaphycus alvarezii. CTU Journal of Innovation and Sustainable Development , 11(2), 22-30. https://doi.org/10.22144/ctu.jen.2019.020
Issue
Vol. 11 No. 2 (2019)

Main Article Content

Abstract

Herein the extraction method of fucoidan from Kappaphycus alvarezii algae is reported. The fresh algae was collected from Dam Mon area (Khanh Hoa province). It was dried to 9.8% moisture and seived to 1mm in homogeneous size. Ethanol 80% was used to exclude pigments and lipids. The conditions of solvent, temperature, time for fucoidan extraction was studied. Fucoidan from Kappaphycus alvarezii was extracted by HCl, solvent rate 1/40 (w/v), 800C during 3 hours at once. The content of fucoidan extract was 40.5µg/ml. The response surface methodology (RSM) was applied to optimize 3 factors of mateial/solvent ratio, temperature and time for the extraction. The optimal results was HCl 1.39M at 85.10C in 3.64 hours, fucoidan content was 47.9 µg/ml. The FT-IR spectrum of fucoidan contain specific peaks of sulfate group in fucoidan in axial or equatorial position.
Keywords: Fucoidan, Kappaphycus alvarezii, HCl, optimazing, RMS

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

References

Ale, M.T., Mikkelsen, J.D., and Meyer, A.S., 2011. Important determinants for fucoidan bioactivity: A critical review of structure-function relations and extraction methods for fucose-containing sulfated polysaccharides from brown seaweeds. Marine drugs. 9(10): 2106-2130.

Ale, M.T., Mikkelsen, J.D. and Meyer, A.S., 2012. Designed optimization of a single-step extraction of fucose-containing sulfated polysaccharides from Sargassum sp. Journal of applied phycology. 24(4): 715-723.

Black, W., Dewar, E., and Woodward, F., 1952. Manufacture of algal chemicals. IV—laboratory‐scale isolation of fucoidin from brown marine algae. Journal of the Science of Food and Agriculture. 3(3): 122-129.

Bilan, M.I., Grachev, A.A., Ustuzhanina, N.E., Shashkov, A.S., Nifantiev, N.E., and Usov, A.I., 2002. Structure of a fucoidan from the brown seaweed Fucus evanescens C. Ag. Carbohydrate research. 337(8): 719-730.

Bilan, M. I., Grachev, A. A., Ustuzhanina, N. E., Shashkov, A. S., Nifantiev, N. E., & Usov, A. I., 2004. A highly regular fraction of a fucoidan from the brown seaweed Fucus distichus L. Carbohydrate research. 339(3): 511-517.

Caporaso, N., Genovese, A., Burke, R., Barry-Ryan, C., and Sacchi, R., 2016. Effect of olive mill wastewater phenolic extract, whey protein isolate and xanthan gum on the behavior of olive O/W emulsions using response surface methodology. Food Hydrocolloids, 61: 66-76.

Dunstan, D.E., Chen, Y., Liao, M.L., Salvatore, R., Boger, D.V., and Prica, M., 2001. Structure and rheology of the κ-carrageenan/locust bean gum gels. Food Hydrocolloids, 15(4): 475-484.

Fayaz M, Namitha KK, Chidambara Murthy KN, Mahadeva Swamy M, Sarada R, Khanam S, Subbarao PV, Ravishankar GA, 2005. Chemical composition, iron bioavailability, and antioxidant activity of Kappaphycus alvarezii (Doty). Journal of Agricultural Food Chem 53(3): 792-797.

Fitton, J.H., 2011. Therapies from fucoidan; multifunctional marine polymers. Marine drugs. 9(10): 1731-1760.

Hahn, T., Lang, S., Ulber, R., and Muffler, K., 2012. Novel procedures for the extraction of fucoidan from brown algae. Process biochemistry. 47(12): 1691-1698.

Joglekar, A. M., May, A. T., Graf, E., & Saguy, I., 1987. Product excellence through experimental design. Food Product and Development: From Concept to the Marketplace, 211-230.

Kawamoto, H., Miki, Y., Kimura, T., et al., 2006. Effects of fucoidan from Mozuku on human stomach cell lines. Food science and technology research. 12(3): 218-222.

Kraan, S., 2012. Algal polysaccharides, novel applications, and outlook, in Carbohydrates-comprehensive studies on glycobiology and glycotechnology. Intech Open. 22: 489-532

Lakmal, H.C., Lee, J.H., and Jeon, Y.-J., 2014. Enzyme-assisted extraction of a marine algal polysaccharide, fucoidan, and bioactivities. Polysaccharides: Bioactivity and Biotechnology. 68: 1-11.

Luo, D., Zhang, Q., Wang, H., et al., 2009. Fucoidan protects against dopaminergic neuron death in vivo and in vitro. European Journal of Pharmacology. 617(1-3): 33-40.

Marais, M.-F., and Joseleau, J.-P., 2001. A fucoidan fraction from Ascophyllum nodosum, Carbohydrate Research. 336(2): 155-159.

Marxen K., Vanselow K.H., Lippemeier S., Hintze R., Ruser A., and Hansen U.-P., 2007, Determination of DPPH radical oxidation caused by methanolic extracts of some microalgal species by linear regression analysis of spectrophotometric measurements, Sensors, 7:2080-2095.

May N.V., 2012. Techniques for drying agricultural products, Ha Noi University of Natural Science. Ha Noi, 234 pages.

Olesya S., Vishchuk L.L, Ermakova S.P., Zvyagintseva T.N, 2011, Sulfated polysaccharides from brown seaweeds Saccharina japonica and Undaria pinnatifida: isolation, structural characteristics, and antitumor activity, Carbohydrate Research 346:2769–2776.

Park, Y., Jang, D. and Kim, S., 1997. Utilization of Marine Products. 2nd edition. Marine product, Hyoungsul Press, pp. 944-966.

Ponce, N.M., Pujol, C.A., Damonte, E.B., Flores, M.L., and Stortz, C.A., 2003. Fucoidans from the brown seaweed Adenocystis utricularis: extraction methods, antiviral activity, and structural studies. Carbohydrate Research. 338(2): 153-165.

Ponce A.G., Roura S.I., del Valle C.E., and Moreira M. R., 2008. Antimicrobial and antioxidant activities of edible coatings enriched with natural plant extracts: in vitro and in vivo studies. Postharvest biology and technology, 49: 294-300.

Rodriguez-Jasso, R. M., Mussatto, S. I., Pastrana, L., Aguilar, C. N., and Teixeira, J. A., 2011. Microwave-assisted extraction of sulfated polysaccharides (fucoidan) from brown seaweed. Carbohydrate Polymers. 86(3): 1137-1144.

Rodríguez-Jasso, R. M., Mussatto, S. I., Pastrana, L., Aguilar, C. N., and Teixeira, J. A., 2013. Extraction of sulfated polysaccharides by autohydrolysis of brown seaweed Fucus vesiculosus, Journal of applied phycology. 25(1): 31-39.

Synytsya, A., Kim, W.J., Kim, S.M., et al., 2010. Structure and antitumor activity of fucoidan isolated from sporophyll of Korean brown seaweed Undaria pinnatifida. Carbohydrate Polymers. 81(1): 41-48.

Sethi, P., 2012. Biochemical composition of marine brown algae, Padina tetrastromatica hauck.

International Journal of Current Pharmaceutical Research. 4(2): 117-118.

Shanthi, N., Eluvakkal, T., and Arunkumar, K., 2014. Characterization of galactose rich fucoidan with anticoagulation potential isolated from Turbinaria decurrens Bory de Saint-Vincent occurring along the coast of Gulf of Mannar (Pamban). J. Pharmacogn. Phytochem. 3: 132-137.

Thi, T.T.V. and Hieu, L.T., 2013. Quality parameters of fucoidan and several products isolated from (Sargassum) from Hue province. Hue University Journal of Science (HU JOS). 74(5): 223-232.

Usov, A., Smirnova, G., and Klochkova, N., 2001. Polysaccharides of algae: 55. Polysaccharide composition of several brown algae from Kamchatka. Russian Journal of Bioorganic Chemistry. 27(6): 395-399.

Vishchuk O. S., Ermakova S. P., and Zvyagintseva T. N., 2011. Sulfated polysaccharides from brown seaweeds Saccharina japonica and Undaria pinnatifida: isolation, structural characteristics, and antitumor activity. Carbohydrate research. 346(17): 2769-2776.

Wang J., Liu L., Zhang Q., Zhang Z., Qi H., and Li P., 2009. Synthesized over sulfated, acetylated and benzoylated derivatives of fucoidan extracted from Laminaria japonica and their potential antioxidant activity in vitro. Food Chemistry. 14: 1285-1290.

Wang, C.-Y. and Chen, Y.-C., 2016. Extraction and characterization of Fucoidan from six brown macroalgae. Journal of Marine Science and Technology. 24(2): 319-328.

Yang, C., Chung, D., Shin, I.S., et al., 2008. Effects of molecular weight and hydrolysis conditions on anticancer activity of fucoidans from sporophyll of Undaria pinnatifida. International journal of biological macromolecules. 43(5): 433-437.

Yuan H., Song J., Li X., Li N., and Liu S., 2011. Enhanced immunostimulatory and antitumor activity of different derivatives of k-carrageenan oligosaccharides from Kappaphycus striatum. J Appl Phycol 23(1), 59-65.

Yuan, Y., and Macquarrie, D., 2015. Microwave-assisted the extraction of sulfated polysaccharides (fucoidan) from Ascophyllum nodosum and its antioxidant activity. Carbohydrate polymers. 129: 101-107.

Zayed, A., Muffler, K., Hahn, T., et al., 2016. Physicochemical and biological characterization of fucoidan from Fucus vesiculosus purified by dye affinity chromatography. Marine drugs. 14(4): 79.