Study on the optimal conditions for fucoidan extraction from Kappaphycus alvarezii

Hoang Thi Ngoc Nhon * , Dinh Thi Huyen and Nguyen Van Nguyen Thinh

* Corresponding author (hoangthingocnhon1002@gmail.com)

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Received: 14 Jan 2019
Revised: 06 Jun 2019
Accepted: 30 Aug 2019
Published: 31 Jul 2019
DOI: 10.22144/ctu.jen.2019.020
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Hoang, T. N. N., Dinh, T. H., & Nguyen, V. N. T. (2019). Study on the optimal conditions for fucoidan extraction from Kappaphycus alvarezii. CTU Journal of Innovation and Sustainable Development , 11(2), 22-30. https://doi.org/10.22144/ctu.jen.2019.020
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Vol. 11 No. 2 (2019)

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Abstract

Herein the extraction method of fucoidan from Kappaphycus alvarezii algae is reported. The fresh algae was collected from Dam Mon area (Khanh Hoa province). It was dried to 9.8% moisture and seived to 1mm in homogeneous size. Ethanol 80% was used to exclude pigments and lipids. The conditions of solvent, temperature, time for fucoidan extraction was studied. Fucoidan from Kappaphycus alvarezii was extracted by HCl, solvent rate 1/40 (w/v), 800C during 3 hours at once. The content of fucoidan extract was 40.5µg/ml. The response surface methodology (RSM) was applied to optimize 3 factors of mateial/solvent ratio, temperature and time for the extraction. The optimal results was HCl 1.39M at 85.10C in 3.64 hours, fucoidan content was 47.9 µg/ml. The FT-IR spectrum of fucoidan contain specific peaks of sulfate group in fucoidan in axial or equatorial position.
Keywords: Fucoidan, Kappaphycus alvarezii, HCl, optimazing, RMS

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