Optimization of the polyphenolics extraction from red rice bran by response surface methodology

Phan Thi Bich Tram * , Duong Thi Phuong Lien and Nguyen Tri Liem

* Corresponding author (ptbtram@ctu.edu.vn)

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Received: 23 May 2018
Revised: 01 Jul 2018
Accepted: 03 Aug 2018
Published: 24 Aug 2018
DOI: 10.22144/ctu.jsi.2018.098
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Phan, T. B. T., Duong, T. P. L., & Nguyen, T. L. (2018). Optimization of the polyphenolics extraction from red rice bran by response surface methodology. CTU Journal of Innovation and Sustainable Development , 54(Special issue on Agriculture), 79-87. https://doi.org/10.22144/ctu.jsi.2018.098
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No. Special issue on Agriculture (2018)

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Abstract

Recently, the interest towards phenol compounds has progressed. In this study, a response surface methodology (RSM) was applied to predict the optimum conditions for extraction of phenolic from red rice bran. Firstly, the effects of ethanol concentration (20–80%), added acetic acid concentration (0–20%), extraction time (0–6.5 hours), temperature (25–100oC), number of extraction cycles (1–4) and solid–solvent ratio (1/4–1/10 w/v) on total polyphenolic content (TPC) of red rice bran were found. After that, three parameters such as solvent concentration (48–68%), added acetic acid concentration (10–15%) and extraction time (120–240 min) were able to be optimized using the Box Behnken design (BBD) with a quadratic regression model built by using RSM. The experiment was designed according to 30 runs with 2 blocks; each block consists of 3 central points and 1 replicate. This design was set up for 3 factors to optimise the response in term of TPC extraction. The extracts were analyzed the TPC spectrophotometrically according to the Folin-Ciocalteu colorimetric assay. The optimal extraction conditions were determined as follows: ethanol concentration of 54.5%, added acetic acid concentration of 13.1%, extraction time of 210 min, temperature of 40oC, 3 cycles of extraction and solid–solvent ratio 1/6 w/v. Using these extraction conditions, the experimental yield of TPC was 2391.1±5.9 mg GAE/100 g dry weight (dw) that was in close significant agreement with predicted value (p < 0.05). The experimental results were fitted to a second order quadratic polynomial model, and they have shown a good fit to the proposed model (R2 = 0.99). With these conditions, the antioxidant capacity assayed by 1,1–diphenyl–2–picrylhydrazyl (DPPH) radical–scavenging activity in term of IC50 value of extract was 108.1±2.9 μg dw/mL. The study result indicates the suitability of the developed model and the success of RSM in optimizing the extraction conditions.
Keywords: Antioxidant activity, extraction, phenolic compounds, red rice bran, response surface methodology

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