Effect of germination on antioxidant capacity and nutritional quality of soybean seeds (Glycinemax (L.) Merr.)

Ha Thanh Toan , Duong Thi Phuong Lien * and Phan Thi Bich Tram

* Corresponding author (dtplien@ctu.edu.vn)

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Received: 09 Oct 2016
Revised: 10 May 2017
Accepted: 29 Jul 2017
Published: 31 Jul 2017
DOI: 10.22144/ctu.jen.2017.032
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Ha, T. T., Duong, T. P. L., & Phan, T. B. T. (2017). Effect of germination on antioxidant capacity and nutritional quality of soybean seeds (Glycinemax (L.) Merr.). CTU Journal of Innovation and Sustainable Development , (06), 93-101. https://doi.org/10.22144/ctu.jen.2017.032
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No. 06 (2017)

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Abstract

The purpose of the current study was to investigate the effects of germination for 0, 12, 24, 36, 42, 48, 60 and 72 hours on nutritional and antioxidative characteristics of germinated soybean seeds (Glycine Max L. Merr.). After germination at 25oC in dark condition, germinated seeds were freeze dried and used for determination the nutritional as well as antinutritional components such as protein, SDS-PAGE protein patterns, fat, phytic acid (PA) and trypsin inhibitor (TI). In addition, the antioxidant capacity of germinated soybean seeds, namely total phenolic content (TPC), total flavonoid content (TFC), vitamin C, α–tocopherol and DPPH radical scavenging activity in terms of IC50 were detected. Results indicated that germination caused significant (P≤ 0.05) increases in protein content. The SDS-PAGE patterns showed the proteins in the samples during 60 hours of germination were unchanged. However, oil content, antinutritional factors (PA and TI) significantly (P≤ 0.05) decreased. It was found that TPC, TFC, vitamin C and α–tocopherol increased during germination and tended to reach the maximum values when  germinated for 60 hours. Germinated seeds had lower IC50 or higher antioxidant capacity. So the present study revealed that germination significantly affects the nutritional and antioxidant properties of soybeans.
Keywords: germination, IC50 value, phytic acid, soybean, trypsin inhibitor

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