Doan Thi Kieu Tien , Lu Chi Thong , Pham Van Luc and Nguyen Thi Thu Nga *

* Correspondence: Nguyen Thi Thu Nga (email:

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The objective of the study was selection promising bacteriophages for lysis Ralstonia solanacearum in vitro and evaluation their ability to prevent bacterial vascular wilt on eggplants under greenhouse conditions. Primary selection of promising bacteriophages from four bacteriophages as Φ54, Φ60, Φ67, and ΦBT on Ralstonia solanacearum isolated from eggplant based on plaque diameter and phage multiplication in in vitro, the result found that three bacteriophages (Φ54, Φ67, and ΦBT) expressed plaque diameter over 7.00 mm at 48 hours, and phage titer with log (pfu/ml) reach over 7.00. Continuing the survey, the efficacy of these phages in controlling bacterial wilt on eggplants in greenhouse conditions through applying each bacteriophage or cocktail of three phages suspension two times (1010 pfu/ pot/ each time) before pathogen inoculation and 7 days after pathogen inoculation through soil drenching.  As a result, all bacteriophages either single or mixture of three phages were effective in the prevention of bacterial wilt disease. Especially, phage Φ BT showed the highest disease reduction and was better than bacteriocide treatment applied with Starner 20 WP.

Keywords: Bacteriophage, eggplant, phage cocktail, Ralstonia solanacearum

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Aoun, N., Tauleigne, L., Lonjon, F., Deslandes, L., Vailleau, F., Roux, F., & Berthomé, R. (2017). Quantitative disease resistance under elevated temperature: genetic basis of new resistance mechanisms to Ralstonia solanacearum. Frontiers in Plant Science, 8, 1387.

Balogh, B., Jones, J. B., Iriarte, F. B., & Momol, M. T. (2010). Phage therapy for plant disease control. Current Pharmaceutical Biotechnology, 11(1), 48-57.

Fujiwara, A., Fujisawa, M., Hamasaki, R., Kawasaki, T., Fujie, M., & Yamada, T. (2011). Biocontrol of Ralstonia solanacearum by treatment with lytic bacteriophages. Applied and Environmental Microbiology, 77(12), 4155-4162.

Jassim, S. A., & Limoges, R. G. (2014). Natural solution to antibiotic resistance: bacteriophages ‘The Living Drugs’. World Journal of Microbiology and Biotechnology, 30(8), 2153-2170.

Laanto E., Mäkelä, K., Hoikkala, V., Ravantti, J. J., & Sundberg, L. R. (2020). Adapting a phage to combat phage resistance. Antibiotics, 9(6), 291.)

Landry, D., González‐Fuente, M., Deslandes, L., & Peeters, N. (2020). The large, diverse, and robust arsenal of Ralstonia solanacearum type III effectors and their in planta functions. Molecular Plant Pathology, 21(10), 1377-1388.

Nobrega, F. L., Vlot, M., de Jonge, P. A., Dreesens, L. L., Beaumont, H. J., Lavigne, R., ... & Brouns, S. J. (2018). Targeting mechanisms of tailed bacteriophages. Nature Reviews Microbiology, 16(12), 760-773.

Peeters, N., Guidot, A., Vailleau, F., & Valls, M. (2013). Ralstonia solanacearum, a widespread bacterial plant pathogen in the post‐genomic era. Molecular Plant Pathology, 14(7), 651-662.

Torres-Barceló, C. (2018). Phage therapy faces evolutionary challenges. Viruses, 10(6), 323.