Uyen Thanh Le , Doan Thi Kieu Tien , Ngoc Tam Huynh , Lan Phuong To and Nguyen Thi Be Nhanh *

* Correspondence: Nguyen Thi Be Nhanh (email:

Main Article Content


Bacterial leaf spots (Xanthomonas spp.) on roses cause great losses to farming. In vitro, the survey of antagonistic density was tested by using three antagonistic isolates (BR16, BR37, BR88) against Xanthomonas spp. (XR13, XR9, XR18 strains). These antagonists and pathogens were diluted separately at 10, 100, 1000 &10000 fold. Results showed that the inhibition abilities were increased with diminishing the population of the pathogen. In which, the antagonists with populations less than 106 CFU/mL are not sufficiently or low effective in forming the inhibitory zone against the pathogen. Therefore, a density of about 107 CFU/mL was selected in the trials on rose under net-house conditions. From that, with the foliar application, three isolates (BR16, BR37, BR88) were tested against the three mentioned pathogenic strains. Results showed that pretreating with antagonistic bacteria achieved high disease control efficiency. In which, BR88 has the highest disease reduction efficiency reaching 70.1%, 72.4%, and 73.3%, when infecting the XR13, XR9, or XR18, respectively. Furthermore, treatments from applying separately three antagonistic isolates all have AUDPC lower than the treatment with only disease inoculation from 2.4 to 4.7 times. In which, the AUDPC of treatment of BR88 was similar to BR16 when infecting XR13,...

Keywords: AUDPC, Antagonistic bacteria, Disease reduction efficiency, Rose, Xanthomonas spp.

Article Details


Abbott, W. S. (1925). A method for computing the effectiveness of an insecticide. Journal of econmic entomology, 18, 265–269.

Cuc, N.T.T., & Thuy, T.T.T. (2014). Epidemiology on rose, chrysanthemum, apricot blossom, marigold (1st ed.). Can Tho University Publisher.

Fira, D., Dimkić, I., Berić, T., Lozo, J., & Stanković, S. (2018). Biological control of plant pathogens by Bacillus species. Journal of Biotechnology, 285, 44–55.

Huang, C.-H., Vallad, G.E., Adkison, H., Summers, C., Margenthaler, E., Schneider, C., Hong, J., Jones, J. B., Ong, K., & Norman, D.J. (2013). A novel Xanthomonas sp. causes bacterial spot of rose (Rosa spp.). Plant Disease, 97, 1301-1307.

Huang, T.-P., Tzeng, D.D.-S., Wong, A.C.L., Chen, C.-H., Lu, K.-M., Lee, Y.H., Huang, W.-D., Hwang, B.-F., & Tzeng, K.-C. (2012). DNA polymorphisms and biocontrol of Bacillus antagonistic to citrus bacterial canker with indication of the interference of phyllosphere biofilms. PLoS ONE, 7, e42124.

International Rice Research Institute. (2002). Standard evaluation system for rice.

Jeger, M. J., & Viljanen-Rollinson, S. L. H. (2001). The use of the area under the disease-progress curve (AUDPC) to assess quantitative disease resistance in crop cultivars. Theoritical and Applied Genetic, 102(1), 32–40.

Lanna - Filho, R., Souza, R. M., Magalhães, M. M., Villela, L., Zanotto, E., Ribeiro-Júnior, P. M., & Resende, M. L. V. (2013). Induced defense responses in tomato against bacterial spot by proteins synthesized by endophytic bacte­ria. Tropical Plant Pathology, 38, 295-302. -56762013005000011

Li, S. B., Fang, M., Zhou, R.C., & Huang, J. (2012). Characterization and evalu­ation of the endophyte Bacillus B014 as a potential biocontrol agent for the control of Xanthomonas axonopodis pv. dieffenbachiae—Induced blight of Anthurium. Biological Control, 63, 9-16.

Liu, K., Garret, C., Fadamiro, H., & Kloepper, J. W. (2016). Antagonism of black rot in cabbage by mixture of plant growth-promoting rhizobacteria (PGPR). Biological Control, 61, 605-613. https://doi. org/10.1007/s 10526-016-9742-3

Sharma, P.D. (2004). Plant pathology (2nd ed.). Rastogi Publicaton.

Singh, D., Dhar, S., & Yadav, D. K. (2010). Effect of endophytic bacterial antago­nists against black rot disease of cauliflower caused by Xanthomonas campestris pv. campestris. Indian Phytopathol, 63(2), 122-126

Maheshwari, D. K. (editor) (2013). Bacteria in Agrobiology: Disease Management (1st ed.), Springer-Verlag Berlin Heidelberg.