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The somatic chromosome number of Panax vietnamensis Ha et Grushv. was determined to be 2n = 24, based on the hypotonic shock method by potassium chloride solution. In this study, we investigated the effect of potassium chloride and colchicine solutions on chromosome dispersion of Panax vietnamensis at different concentrations. The treatment using 0.2% KCl solution in 45 minutes combined with 0.05% colchicine solution in 2 hours subsequently resulted in proper hypotonia. The result showed that chromosomes were evenly dispersed. The hypotonic shock method seemed to be effective in equally distributing chromosomes. The result can be applied in cell genetic studies and selective breeding programs for Panax vietnamensis.
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Fukui, K., & Iijima, K. (1991). Somatic chromosome map of rice by imaging methods. Theoretical and Applied Genetics, 81(5), 589-596.
Ha T. D., & Grushvisky, I. V. (1985). A new species of the genus Panax L. Araliaceae in Vietnam: Panax vietnamensis Ha et Grushv. Bot J Vietnam, 70, 518-22.
Levan, A., 1938. The effect of colchicine on root mitoses in Allium. Hereditas, 24(4), 471-486.
Manzanilla, V., Kool, A., Nguyen, N. L., Nong, V. H., Le, T. T. H., & De Boer, H. J. (2018). Phylogenomics and barcoding of Panax: toward the identification of ginseng species. BMC evolutionary biology, 18(1), 1-14.
Nguyen, B., Kim, K., Kim, Y. C., Lee, S. C., Shin, J. E., Lee, J., ... & Yang, T. J. (2017). The complete chloroplast genome sequence of Panax vietnamensis Ha et Grushv (Araliaceae). Mitochondrial DNA Part A, 28(1), 85-86.
Phan, K. L., Vu, D. D., Phan, K. L., Nguyen, G. S., Nguyen, T. P. T., Le, T. M. L., & Le, T. S. (2014). Phylogenetic relationships of the Panax samples collected in Lai Chau Province based on MATK and ITS-rDNA sequences. J. Biotechnol. (Vietnam), 12(2), 327-337.
Vo, T. T. P. (2012). A study of chromosome number in animal cells and plant cells by hypotonic shock treatment. Can Tho University Journal of Science. (21b), 198-208.
Yi, T., Lowry, P. P., Plunkett, G. M., & Wen, J. (2004). Chromosomal evolution in Araliaceae and close relatives. Taxon, 53(4), 987-1005.
Zhang, D., Li, W., Xia, E. H., Zhang, Q. J., Liu, Y., Zhang, Y., ... & Gao, L. Z. (2017). The medicinal herb Panax notoginseng genome provides insights into ginsenoside biosynthesis and genome evolution. Molecular Plant, 10(6), 903-907.
Zhu, S., Fushimi, H., Cai, S., Chen, H., & Komatsu, K. (2003). A new variety of the genus Panax from southern Yunnan, China, and its nucleotide sequences of 18S ribosomal RNA gene and matK gene. Journal of Japanese Botany, 78(2), 86-94.