Huynh Xuan Phong , Huynh Nguyen Nhu Thu * , Bui Hoang Dang Long , Takeshi Zendo , Kenji Sonomoto and Ngo Thi Phuong Dung

* Correspondence: Huynh Nguyen Nhu Thu (email:

Main Article Content


The objectives of this study were to select a number of thermotolerant lactic acid bacteria for their application in biomass production at high temperature and to study the genetic relation of these selected strains by using 16S ribosomal DNA sequences. All 16 tested strains of thermotolerant LAB were found to possess the antibacterial ability and the capability of bacteriocin production against Bacillus subtilis. As a result, all 16 LAB strains had an antibacterial ability and produced bacteriocin against indicator. Ten selected strains having the strongest antibacterial ability were identified as Lactobacillus plantarum, L. casei, and L. delbrueckii. The L. plantarum L54 was selected for the experiment of the optimum conditions for biomass production because of its strongest antibacterial ability with the diameter of inhibitory zone in “agar spot test” and “well-diffusion agar” were 13.76 mm and 17.33 mm, respectively. Based on statistical analysis, the optimum conditions for biomass production by L. plantarum L54 at 39°C were 5.99% (w/v) of glucose concentration, 6.37% (v/v) of bacterial inoculum concentration, and pH 6.0.
Keywords: Antibacterial activity, biomass, lactic acid bacteria, Lactobacillus plantarum, thermotolerant

Article Details


Axelsson L., 2004. Lactic acid bacteria: Classification and physiology. In: Salminen, S., von Wright, A., Ouwehand, A., (Eds). Lactic Acid Bacteria: Microbiological and Functional Aspects. Third Edition. New York, NY: Marcel Dekker; 1-66.

Andersen, A.Z., Carvalho, A.L., Neves, A.R., Santos, H., Kummer, U., and Olsen, L.F., 2009. The metabolic pH response in Lactococcus lactis: An integrative experimental and modeling approach. Computational Biology and Chemistry. 33(1): 71-83.

Bai, D.M., Wei, Q., Yan, Z.H., Zhao, X.M., Li, X.G., and Xu, S.M., 2003. Fed batch fermentation of Lactobacillus lactis for hyper-production of L-lactic acid. Biotechnology Letters. 25(1): 1833-1835.

Bui Hoang Dang Long, 2016. Selection of thermotolerant lactic acid bacteria and application in lactic acid fermentation. Graduate Thesis of Biotechnology, Can Tho University, Vietnam (in Vietnamese).

Calabia, B.P., Tokiwa, Y., and Aiba, S., 2011. Fermentative production of L-(+)-lactic acid by an alkaliphilic marine microorganism. Biotechnology Letters. 33(2): 1429-1433.

Cleveland, J., Chiknids, M. and Montiville, T.J., 2002 Multimethod assessment of commercial nisin preparations. Journal of Industrial Microbiology and Biotechnology. 29(1): 228-232.

De Man, J.C., Rogosa, M., and Sharpe, M.E., 1960. A medium for the cultivation of Lactobacilli. Journal of Applied Bacteriology. 23: 130-135.

Dung, N.T.P. and Phong, H.X., 2011. Optimal conditions for bacteriocin production by lactic acid bacteria using cheap medium of tofu sour liquid and brewer’s grains. The 4th International Conference on Fermentation Technology for Value Added Agricultural Products. Khon Kaen, Thailand, p.116.

Elmarzugi, N., Enshasy, H.E., Malek, R.A., Othman, Z., Sarmidi, M.R., and Aziz, R.A., 2010. Optimization of cell mass production of the probiotic strain Lactococcus lactis in batch and fed-bach culture in pilot scale levels. Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology. 2: 873-879.

Herna´ndez, D., Cardell, E., and Za´rate, V., 2014. Antimicrobial activity of lactic acid bacteria isolated from Tenerife cheese: Initial characterization of plantaricin TF711, a bacteriocin-like substance produced by Lactobacillus plantarum TF711. Journal of Applied Microbiology. 99: 77-84.

Liu, B., Yang, M., Qia, B., Chen, X., Su, Z., and Wana, Y., 2010. Optimizing L-(+)-lactic acid production by thermophile Lactobacillus plantarum As.1.3 using alternative nitrogen sources with response surface method. Biochemical Engineering Journal. 52: 212-219.

Lu Nguyen Bich Ngoc, 2014. Isolation and selection of thermotolerant lactic acid bacteria in agriculture waste. Undergraduate Thesis of Biotechnology, Can Tho University, Vietnam.

Merih, K., Yilmaz, M., Carik, E., 2009. Isolation and identification of lactic acid bacteria from boza, and their microbial activity against several reporter strains. Turkish Journal of Biology. 35: 313-324.

Niamsup, P., Sujaya, I.N., Tanaka, M., et al., 2003. Lactobacillus thermotolerans sp. nov., a novel thermotolerant species isolated from chicken faeces. International Journal of Systematic and Evolutionary Microbiology. 53(1): 263-268.

Ouwehand, A.C., 1998. Antimicrobial components from lactic acid bacteria. Marcel Dekker Inc., New York, 139-159.

Onda, T., Yanagida, F., Sufi, M.T., Ogino, S., and Shinohara, T., 1999. Isolation and characterization of lactic acid bacteria strain GM005 producing antibacterial substance from Miso-paste product. Food Science Technology. 5(3): 247-250.

Piard, J.C. and Desmazeaud, M., 1991. Inhibiting factors produced by lactic acid bacteria: Oxygen metabolites and catabolism endproducts. Le Lait Dairy Science and Technology. 71: 525-541.

Sudiyani, Y., Syarifah, A., Yulia, A. and Indri, B.A., 2007. Characterization of waste water from tofu industry. International Conference on Chemical Sciences (ICCS-2007), ANL/47-6.

Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S., 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution. 30(12): 2725-2729.

William, G., Susan, M.B., Dale, A.P., and David, J.L., 1991. 16S Ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology. 173 (2): 697-703.

Ying, R.S. and Meng, Y.K., 2017. Effects of different carrageenan types on the rheological and water-holding properties of tofu. LWT - Food Science and Technology. 78: 122-128.