Ethanol production from molasses at high temperature by thermotolerant yeasts isolated from cocoa

Huynh Xuan Phong * , Pornthap Thanonkeo , Mamoru Yamada , Nguyen Tran Cam Giang , Sukanya Nitiyon and Ngo Thi Phuong Dung

* Corresponding author (hxphong@ctu.edu.vn)

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Received: 18 Aug 2015
Revised: 27 Jan 2016
Accepted: 08 Aug 2016
Published: 19 Aug 2016
DOI: 10.22144/ctu.jen.2016.021
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Huynh, X. P., Pornthap, T., Mamoru, Y., Nguyen, T. C. G., Sukanya, N., & Ngo, T. P. D. (2016). Ethanol production from molasses at high temperature by thermotolerant yeasts isolated from cocoa. CTU Journal of Innovation and Sustainable Development , (03), 32-37. https://doi.org/10.22144/ctu.jen.2016.021
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No. 03 (2016)

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Abstract

Thermotolerant ethanologenic yeasts have highly attracted many scientists due to the current challenges of increasing global temperature and the benefits associated with processing at high temperature as well as reducing cooling cost. In this study, 50 yeast strains were isolated from 35 different materials of cocoa. Based on characteristics of morphology, physiology and biochemistry, 50 yeast isolates were preliminarily classified into genera as Candida, Hanseniaspora, Kluyveromyces, Pichia, and Saccharomyces. There were 17/50 isolates showing their ethanol tolerant ability up to 12% (v/v) of ethanol and 23/50 isolates could grow at 45oC. The two selected isolates (CT2.5D and PD1.6H) were tested for the ethanol fermentation from molasses (22°Brix) at different temperatures. The results of ethanol concentrations obtained as follow: 7.36% (v/v) at 30oC, 4.15% (v/v) at 40oC, 1.45% (v/v) at 42oC by CT2.5D and 7.4% (v/v) at 30oC, 2.93% (v/v) at 40oC, 1.43% (v/v) at 42oC by PD1.6H. The identification results showed that CT2.5D and PD1.6H were Saccharomyces cerevisiae.
Keywords: cocoa, ethanol fermentation, ethanol tolerance, Saccharomyces cerevisiae, thermotolerant yeast

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