Optimization of polyphenol, flavonoid and tannin extraction conditions from Pouzolzia zeylanica L. benn using response surface methodology

Nguyen Duy Tan * , Le Quoc Viet , Vo Tan Thanh and Nguyen Minh Thuy

* Corresponding author (ndtan@agu.edu.vn)

Article Sidebar

Full Text: PDF

Received: 27 Jan 2016
Revised: 27 Apr 2016
Accepted: 30 Mar 2017
Published: 31 Mar 2017
DOI: 10.22144/ctu.jen.2017.015
Views
642
Downloads
161
Crossref
Scopus
Google Scholar
Europe PMC
How to Cite
Nguyen, D. T., Le, Q. V., Vo, T. T., & Nguyen, M. T. (2017). Optimization of polyphenol, flavonoid and tannin extraction conditions from Pouzolzia zeylanica L. benn using response surface methodology. CTU Journal of Innovation and Sustainable Development , (05), 122-131. https://doi.org/10.22144/ctu.jen.2017.015
Issue
No. 05 (2017)

Main Article Content

Abstract

In this study, the extraction of phenolic compounds from Pouzolzia Zeylanica L. Benn was conducted by using pure water as a solvent. The optimal conditions for the extraction of three phenolic compounds such as polyphenols, flavonoids and tannins were determined by using response surface methodology (RSM). A central composite design (CCD) was applied to investigate the effects of three independent variables, namely the ratio of water-to-dried material (20:1 to 30:1, v/w), temperature (70 to 90oC ) and time extraction (20 to 40 minutes). The dependent variables were total polyphenol content (TPC), total flavonoid content (TFC) and tannin content (TC). A second-order polynomial model was used for predicting the response. Optimized conditions for bioactive compounds extraction, water-to-dried material ratio, time and temperature extraction were 27 (v/w), 30 minutes and 81oC, respectively. The experimental values agreed with predicted values within a 95% confidence interval. Total polyphenol, flavonoid and tannin content extracted by these optimized conditions were achieved (521 mgGAE/100g dried material (DM), 363 mgQE/100g DM and 643 mgTAE/100g DM, respectively).
Keywords: Extraction, phenolic compounds, pouzolzia zeylanica L. benn, optimization, response surface methodology

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

References

Ahmed, A., Rajendaran, K., Jaiswal, D., Singh, H.P., Mishra, A., Chandra, D., Yadav, I.K., Jain, D.A., 2010. Anti-snake venom activity of different extract of Pouzolzia indica against Russel viper venom. International Journal of Chem. Tech. Research. 2 (1): 744-751.

Bhattacharjya, D.K., Borah, P.C., 2008. Medicinal weeds of crop fields and role of women in rural health and hygiene in Nalbari district, Assam. Indian Journal of Traditional Knowledge. 7 (3): 501-504.

Bonilla, F., Mayen, M., Merida J., Medina, M., 1999. Extraction of phenolic compounds from red grape marc foruse as food lipid antioxidants. Food Chemistry. 66 (2): 209-215.

Cacace, J.E., Mazza, G., 2003. Mass transfer process during extraction of phenolic compounds from milled berries. Food and Engineering. 59: 379-389.

Chi, V.V., 2012. Dictionary medicinal plants in Vietnam. Medicine Publishing House. pp. 189-191 (in Vietnamese).

Connolly, D.L., 1993. Tannin extraction. US Patent. 5: 238-680.

Eswari, M.L., Bharathi, R.V., Jayshree, N., 2013. Preliminary phytochemical screening and heavy metal analysis of leaf extracts of Ziziphus oenoplia (L) Mill. Gard. International Journal of Pharmaceutical Sciences and Drug Research. 5 (1): 38-40.

Fu, M., Niu, Y.Y., Yu, J., Kong Q.T., 2012. Study on the chemical constituents in Pouzolzia zeylanica. Zhong Yao Cai. 35 (11): 1778-17781.

Ghani, 2003. Medicinal plants of Bangladesh: Chemical constituents and uses, 2nd Ed., The Asiatic Society of Bangladesh, Dhaka. pp. 104

Hossain, M.A., Raqmi, K.A.S., Mijizy, Z.H., Weli, A.M., Riyami, Q., 2013. Study of total phenol, flavonoids contents and phytochemical sreening of various leaves crude extracts of locally grown Thymus vularis. Asian Pacific Journal of Tropical Biomedicine. 3 (9): 705-710.

Jayaprakasha, G.K., Singh, R.P., Sakariah, K.K., 2001. Antioxidant activity of grape seed (vitisvinifera) extractson peroxidation models in vitro. Food Chemistry. 73 (3): 285-290.

Laitonjam W.S., Yumnam R., Asem S.D., Wangkheirakpam S.D., 2013. Evaluative and comparative study of biochemical, trace elements and antioxidant activity of Phlogacanthus pubinervius T. Anderson and Phlocanthus jenkincii C.B. Clarke leaves. Indian Journal of Natural Products and Resources. 4 (1): 67-72.

Liu, H., Du, X., Yuan, Q., Zhu, L., 2009. Optimisation of enzyme assisted extraction of silybin from the seeds of silybum marianum by box-behnken experimental design. Phytochemical Analysis. 20 (6): 475-483.

Mandal, S., Patra, A., Samanta, A., Roy, S., Mandal, A., Mahapatra, T.D., Pradhan, S., Das, K., Nandi, D.K., 2013. Analysis of phytochemical profile of Terminalia arjuna bark extract with antioxidative and antimicrobial properties. Asian Pacific Journal of Tropical Biomedicine. 3 (12): 960-966.

Paul, S., Saha, D., 2012. In vitro screening of cytotoxic activities of ethanolic extract of Pouzolzia Zeylanica (L.) Benn. International Journal of Pharmaceutical Innovations. 2 (1): 52-55.

Pinelo, M., Fabbro, P.D., Manzocco, L., Nunez, M.J., Nicoli, M.C., 2005. Optimization of continuous phenol extraction from vitisvinifera byproducts. Food Chemistry. 92 (1): 109-117.

Pinelo, M., Rubilar, M., Jerez, M., Sineiro J., Nunez, M.J., 2005. Effect of solvent, temperature, and solvent-to-solid ratio on the total phenolic content and antiradical activity of extracts from different components of grapepomace. Journal of Agricultural and Food Chemistry. 53 (6): 2111-2117.

Rajha, H.N., Darra, N.E., Louka, N., Maroun, R.G., Ziegler W., Bochzelt, H., 2012. Valorization of industrial waste using energy saving procedures. Phenolic compounds purification from grape by-products by accelerated solvent extraction (ASE). International Conferenceon Renewable Energies for Developing Countries. pp. 1-5.

Ramos, L., Kristenson, E. M., Brinkman,U. A., 2002. Currentuse of pressurised liquid extraction and subcritical water extractionin environmental analysis. Journal of Chromatography A. 975 (1): 3-29.

Ratnam K. V., Raju R. R. V., 2008. Traditional medicine used by the adivasis of Eantern Ghats, Andhra Pradesh – for bone fractures. Ethnobotanical leaflets. 12: 19-22.

Saha, D., Paul, S., 2012a. Studies on Pouzolzia zeylanica (L.) Benn. (Family: Urticaceae). Lap Lambert Academic Publishing, Germany. pp.18-35.

Saha, D., Paul, S., 2012b. Antifungal activity of ethanol extract of Pouzolzia Zeylanica (L.) Benn. International Journal of Pharmacy Teaching and Pratices. 3 (2): 272-274.

Saha, D., Paul, S., Chowdhury, S., 2012. Antibacterial activity of ethanol extract of Pouzolzia Zeylanica (L.) Benn. International Journal of Pharmaceutical Innovations. 2 (1): 1-5.

Sheng, Z.L., Wan, P.F., Dong C.L., Li, Y.H., 2013. Optimizationof total flavonoids content extracted from Flospopuli using response surface methodology. Industrial Crops and Products. 43: 778-786.

Silva, E.M., Rogez H., Larondelle, Y., 2007. Optimizationof extraction of phenolics from Inga edulis leaves using response surface methodology. Separation and Purification Technology. 55 (3): 381-387.

Son V.H., Tu, H.D., 2009. Study on polyphenol extraction from dust green tea. Part 1. The influence factors on polyphenol extraction. Journal of Science and Technology. 47 (1): 81-86 (in Vietnamese).

Spigno, G., De Faveri, D. M., 2007. Antioxidants from grape stalks and marc: influence of extraction procedure on yield, purity and antioxidant power of the extracts. Journal of Food Engineering. 78 (3): 793-801.

Spigno, G., Tramelli L., De Faveri, D.M., 2007. Effects ofextraction time, temperature and solvent on concentration and antioxidant activity of grape marc phenolics. Journal of Food Engineering. 81 (1): 200-208.

Thuy, L.T., 2007. Preliminary test of chemical components for Pouzolzia zeylanica L. Benn. Chemical Master Thesis, University of Natural Science, HCM city (in Vietnamese).

Tien, T.M., Huong, N.T.T., Ngoc, D.T.M., Luan, T.C., Kinh, L.V., 2010. Experimental study on some pharmacological effects of Pouzolzia zeylanica and Tinospora crispa. Medicine Journal of HCM city, special subject Traditional medicine. 14 (2): 116-120 (in Vietnamese).

Tsai, C.W., Tong, L.I., Wang, C.H., 2010. Optimization of multiple responses using data envelopment analysis and response surface methodology.Tamkang Journal of Scienceand Engineering. 13 (2): 197-203.

Yilmaz, Y., Toledo, R.T., 2006. Oxygen radical absorbance capacities of grape/wine industry by products and effect of solvent type on extraction of grape seed polyphenols. Journal of Food Composition and Analysis. 19 (1): 41-48.

Yusuf, M. and Chowdhury, J.U., 1994. Medicinal plants of Bangladesh. 1st ed. Dhaka, Bangladesh Council of Scientific and Industrial Research. pp. 207

Zhu, X.Y., Mang, Y.L., Xie, J., Wang P., Su, W.K., 2011. Response surface optimization of mechanochemical-assisted extraction of flavonoids and terpene trilactones from ginkgo leaves. Industrial Crops and Products. 34 (1): 1041-1052