Rapid detection of Streptococcus iniae in red tilapia tissue (Oreochromis sp.) by polymerase chain reaction

Ngo Minh Phuong * and Tran Thi Tuyet Hoa

* Corresponding authorNgo Minh Phuong

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Received: 29 Jul 2015
Revised: 29 Jul 2015
Accepted: 19 Feb 2016
Published: 28 Feb 2016
DOI: 10.22144/ctu.jen.2016.004
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Ngo, M. P., & Tran, T. T. H. (2016). Rapid detection of Streptococcus iniae in red tilapia tissue (Oreochromis sp.) by polymerase chain reaction. CTU Journal of Innovation and Sustainable Development , (02), 84-89. https://doi.org/10.22144/ctu.jen.2016.004
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No. 02 (2016)

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Abstract

Streptococcus iniae, as a cause of Streptococcosis outbreak of cultured tilapia, is a hemolytic, Gram-positive bacterium. In this study, a one-step PCR method was developed for the detection of S. iniae from fish kidney. The oligonucleotide primers, assigned as LOX-1 and LOX-2, are designed from lactate oxidase gene of S. iniae. Using extracted DNA from bacterial cells or head kidney of S. iniae infected fish, the PCR reaction yields a 870 bp fragment specific to the S. iniae sequence. Primers LOX-1 and LOX-2 did not amplify DNA from other common bacteria in cultured fish (Streptococcus agalactiae, Aeromonas hydrophila, Edwardsiella ictaluri, Vibrio harveyi). The detection limit of this primer pair is 102 CFU/ml of S. iniae. The improved one-step PCR method provides a diagnostic tool for: (i) the detection of S. iniae directly from infected fish tissue; (ii) and the identification of S. iniae from bacteria cell.
Keywords: PCR, Streptococcus iniae, tissue, red tilapia

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