Primary investigating chemical constituents of bioactive extract from Centrostachys aquatica (R.Br.) Wall. ex Moq.-Tand.
Main Article Content
This study is aimed to screen the biological activities and chemical composition to find evidences for potential medicinal applications of Centrostachys aquatica in the Mekong Delta. Crude methanol extract and subextracts in n-hexane, ethyl acetate, and acetone from Centrostachys aquatica were tested bioactivities. The methanol extract, n-hexane and ethyl acetate subextracts exhibited antimicrobial activity with corresponding MIC values of 200, 100 and 200 µg/mL, respectively. The ethyl acetate subextract was inhibited cytotoxicity against cancer cell line LU-1 with IC50 of 27.66 µg/mL. None of the extracts showed antioxidant ability. Three known secondary metabolites including oleanolic acid (1), 20(E)-hydroxy-b-ecdysone (2), and b-spinasterol (3) were isolated for the first time from the bioactive (ethyl acetate) subextract of Centrostachys aquatica. Their structures were elucidated by modern spectra as MS, NMR and comparison with published data.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Bich, T. T. N., & Kato-Noguchi, H. (2014). Isolation and identification of a phytotoxic substance from the emergent macrophyte Centrostachys aquatica. Botanical Studies, 55(59), 1–5. https://doi.org/10.1186/s40529-014-0059-1
Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. L.W.T. Food Science and Technology, 28(1), 25–30. https://doi.org/10.1016/S0023-6438(95)80008-5
Ho, P. H. (1999). “Vietnamese plants”- An illustrated flora of Vietnam. Tre Publisher (in Vietnamese).
Kumar, G. P., Navyaa, K., Ramya, E. M., Venkataramana, M., Anand, T., & Anilakumar, K. R. (2013). DNA damage protecting and free radical scavenging properties of Terminalia arjuna bark in PC-12 cells and plasmid DNA. Free Radicals and Antioxidants, 3, 35–39. https://doi.org/10.1016/j.fra.2013.04.001
Likhitayawuid, K., & Angerhofer, C. K. (1993). Cytotoxic and antimalarial bisbenzylisoquinoline alkaloids from Sephania evecta. Jounal of Natural Products, 56(1), 30–38. https://doi.org/10.1021/np50091a005
Mckane, L., & Kandel J. (1996). Microbiology – Essentials and Applications (2nd ed.). McGraw-Hill Companies.
Meneses-Sagrero, S. E., Navarro-Navarro, M., Ruiz-Bustos, E., Del-Toro-Sánchez, C. L., Jiménez-Estrada, M., & Robles-Zepeda, R. E. (2017). Antiproliferative activity of spinasterol isolated of Stegnosperma halimifolium (Benth, 1844). Saudi Pharmaceutical Journal, 25(8), 1137–1143. https://doi.org/10.1016/j.jsps.2017.07.001
Paszel-Jaworska, A., Romaniuk, A., & Rybczynska, M. (2014). Molecular Mechanisms of Biological Activity of Oleanolic Acid - A Source of Inspiration for A New Drugs Design. Mini-Reviews in Organic Chemistry, 11(3), 330–342. https://doi.org/10.2174/1570193X1103140915111839
Ragasa, C. Y., & Lim, K. (2005). Sterols from Cucurbita maxima. Philippine Journal of Science, 134(2), 83–87.
Raghavan, S. D. A., & Ayanath, A. (2019). Effect of 20-OH ecdysone and methyl farnesoate on moulting in the freshwater crab Travancoriana schirnerae. Invertebrate Reproduction & Development, 1–10. https://doi.org/10.1080/07924259.2019.1653387
Shela, G., Ratiporn, H., Yong-Seo, P., Soon-Teck, J., Zofia, Z., Zenon, J., Elena, K., Simon, T., & Olga, M. B. (2004). Bioactive compounds and antioxidant potential in fresh and dried Jaffa sweeties, a new kind of citrus fruit. Journal of the Science of Food and Agriculture, 84, 1459–1463. https://doi.org/10.1002/jsfa.1800
Skehan, P., Storeng, R., Scudiero, D., Monks, A., McMahon, J., Vistica, D., … & Boyd, M. R. (1990). New colorimetric cytotoxicity assay for anticancer-drug screening. JNCI Journal of the National Cancer Institute, 82(13), 1107–1112. https://doi.org/10.1093/jnci/82.13.1107
Vanden Berghe, D. A., & Vlietinck, A. J. (1991). Screening methods for antibacterial and antiviral agents from higher plants. In K. Hostettmann (ed.), Methods in Plant Biochemistry, Vol. 6, Assays for Bioactivity, Academic Press, London (pp. 47–69).
Vokac, K., Budesnsky, M., Harmatha, J., & Kohoutova, J. (1998). Ecdysteroid constituents of the mushroom Tapinella panuoides. Phytochemistry, 49(7), 2109–2114. https://doi.org/10.1016/S0031-9422(98)00448-8
Zuhal, G., Hilal, O., Ay，se K., Cavit K., & Omur, L. D. (2009). Secondary metabolites from Nepeta heliotropifolia. Turk Journal of Chemistry, 33, 667–675.