Isolation and selection of lactic acid bacteria that can antagonize Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease in whiteleg shrimp (Penaeus vannamei)

Nguyen Thi Truc Linh * , Nguyen Trong Nghia , Dang Thi Hoang Oanh , Huynh Thanh Phong and Truong Quoc Phu

* Corresponding author (truclinh@tvu.edu.vn)

Article Sidebar

Full Text: PDF

Received: 16 Jan 2017
Revised: 08 Mar 2017
Accepted: 31 Oct 2017
Published: 29 Nov 2017
DOI: 10.22144/ctu.jen.2017.052
Views
346
Downloads
135
Crossref
Scopus
Google Scholar
Europe PMC
How to Cite
Nguyen, T. T. L., Nguyen, T. N., Dang, T. H. O., Huynh, T. P., & Truong, Q. P. (2017). Isolation and selection of lactic acid bacteria that can antagonize Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease in whiteleg shrimp (Penaeus vannamei). CTU Journal of Innovation and Sustainable Development , (07), 74-81. https://doi.org/10.22144/ctu.jen.2017.052
Issue
No. 07 (2017)
Section
Fisheries

Main Article Content

Abstract

This study was conducted from March to June, 2015 to select lactic acid bacteria (LAB) strains that can antagonize V. parahaemolyticus for further studies on prevention of acute hepatopancreatic necrosis disease (AHPND) in shrimp. LAB strains were isolated from different resources including: (1) gut of white leg shrimp (Penaeus vannamei); (2) gut of nile tilapia (Oreochromis niloticus) and (3) shrimp pond sediment in Tra Vinh, Ben Tre, and Soc Trang provinces. Isolated LAB strains were identified by using morphological, physiological and bio-chemical characteristics and then determined their antagonism toward V. parahaemolyticus by using agar well diffusion method. A total of 94 LAB strains were selected for this study (30, 39, 25 isolates from Tra Vinh, Ben Tre, Soc Trang provinces, respectively). For antimicrobial activity, 13 strains could weakly antagonize V. parahaemolyticus with inhibition diameter smaller than 11.0 mm. However, 81 remaining LAB strains could antagonize V. parahaemolyticus with inhibition diameter around 11.0–18.5 mm. Of the 94 strains above mentioned, three strains RP6.5, RP5.4.1 and RP5.5.1 had the biggest inhibition diameters (17.3±0.58 mm, 18.5±0.289 mm and 18.00±0.00mm, respectively). These trains can be used for further studies to evaluate the effect of LAB in prevention AHPND in shrimp.
Keywords: AHPND, isolation, lactic acid bacteria, Vibrio parahaemolyticus

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

References

Alessandro, D., Cesar, D.E., and Abreu, P.C., 2015. Bacterial community of pond’s water, sediment and in the guts of tilapia (Oreochromis niloticus) juveniles characterized by fluorescent in situ hybridization technique. Aquaculture Research. 46(3): 707-715.

Ammor, S., Tauveron, G., Dufour, E., and Chevallier, I., 2006. Antibacterial activity of lactic acid bacteria against spoilage and pathogenic bacteria isolated from the same meat small-scale facility: 1-Screening and characterization of the antibacterial compounds. Food Control. 17(6): 454-461.

Ariole, C.N. and Nyeche, G.E., 2013. In vitro antimicrobial activity of Lactobacillus isolates against shrimp (Penaeus monodon) pathogens. International Journal of Biosciences. 3(1): 7-12.

Bernet-Camard, M. F., Lievin, V., Brassart, D., Neeser, J.R., Servin, A.L., and Hudault, S., 1997. The human Lactobacillus acidophilus strain LA1 secretes a nonbacteriocin antibacterial substance(s) active in vitroand in vivo. Applied and Environmental Microbiology. 63: 2747-2753.

Debaere, P., 2010. Small Fish–big Issues: The Effect of Trade Policy on the Global Shrimp Market, World Trade Review. 9(2): 353-374.

FAO, Food and Agriculture Organization, 2013. Report of the FAO/MARD Technical Workshop on Early Mortality Syndrome (EMS) or Acute Hepatopancreatic Necrosis Disease (AHPND) of cultured shrimp (under TCP/VIE/3304). Hanoi, Vietnam, on 25-27 June 2013. FAO Fisheries and Aquaculture Report No. 1053. Rome. 54pages.

Huynh Ngoc Truong, Tran Thi Ngoc Thanh, and Nguyen Tien Dung, 2015. Prevalence and antibiotic resistance pattern of Vibrio spp. isolated from aquaculture and environment in Tien Giang province. Ho Chi Minh Pedagogy University Journal of Science. 2(67): 157-166

Kandler, O. and Weiss, N., 1986. Genus Lactobacillus Beijerinck 1901, 212AL. Bergey’s Manual of Systematic Bacteriology. In: P.H.A. Sneath, N.S. Mair, M.E. Sharpe, J.G. Holt. (Eds.). Baltimore: Williams & Wilkins. vol. 2, pp. 1209-1234.

Khuat Huu Thanh, Nguyen Dang Phuc Hai, and Bui Van Dat, 2009. Separating and selecting some strains of bacteria with probiotic particularity in creating products for shrimp farming. Journal of Science of Ho Chi Minh Teaching University. 2(67): 74-84.

Klayraung, S., Viernstein, H., Sirithunyalug, J., and Okonogi, S., 2008. Probiotic properties of Lactobacilli isolated from Thai traditional food. Scientia Pharmaceutica. 76: 485-503.

Tran Loc, Nunan, L., Redman, R.M., Mohney, L.L., Pantoja, C.R., Fitzsimmons, K., and Lightner, D.V., 2013. Determination of the infectious nature ofthe agent of acute hepatopancreatic necrosis syndrome affecting penaeid shrimp. Diseases of aquatic organisms. 105: 45-55.

Ma, C.W., Cho, Y.S., and Oh, K.H., 2009. Removal of pathogenic bacteria and nitrogens by Lactobacillus spp. JK-8 and JK-11. Aquaculture. 287: 266-270.

Michetti, P., Dorta, G., Wiesel, P.H., et al., 1999. Effect of whey-based culture supernatant of Lactobacillusacidophilus (johnsanii) La1 on Helicobacter pylori infection in humans. Digestion. 60: 203-209.

Ngo Thi Phuong Dung, Huynh Thi Yen Ly, and Huynh Xuan Phong, 2011. Isolation and selection of lactic acid bacteria producing anti-bacterial substances. Can Tho University Journal of Scicence. 19a: 176-184 (in Vietnamese).

Nguyen Trong Nghia, Dang Thi Hoang Oanh, Truong Quoc Phu, and Pham Anh Tuan, 2015. Isolation and determination of the ability to cause acute hepatopancreatic necrosis syndrome of Vibrio parahaemolitycus bacteria isolated from culturedshrimp in Bac Lieu province. Can Tho University Journal of Science. 39: 99-107 (in Vietnamese).

Nguyen Van Minh, Le Anh Tuan, Dao Van Toan, Vo Ngoc Yen Nhi, Duong Nhat Linh, and Nguyen Thi Ngoc Tinh, 2014. Biocontrol of Vibrio parahaemolyticus NT7 isolated from AHPNS whiteleg shrimp disease by Bacillus polyfermenticus F27 isolated from Perionyx excavatus. Ho Chi Minh city, 30-31/1/2015. Proceedings of Workshop in Research and Biotechnological application on agriculture and rural development (in Vietnamese).

Nguyen Van Thanh and Nguyen Ngoc Trai, 2012. Isolation of Lactobacillus sp. inhibitting bacteria causing "red-sore disease" and "white spot in the internal organs" on Pangasianodon hypophthalmus. Can Tho University Journal of Science. 23a 224-234 (in Vietnamese).

Nirunya, B., Suphitchaya, C., and Tipparat, H., 2008. Screening of lactic acid bacteria from gastrointestinal tracts of marine fish for their potential use as probiotics. Songklanakarin journal of science and technology. 30(1): 141-148.

Noordiana N., Fatimah A.B., and Mun, A.S., 2013. Antibacterial agents produced by lactic acid bacteria isolated from Threadfin Salmon and Grass Shrimp. International Food Research Journal. 20(1): 117-124.

Parvathy, S.N., and Surendran., P.K., 2005. Biochemical characterization of lactic acid bacteria isolated from fish and prawn. Jounal of culture collections. 4. pp. 48-52.

Ponce, A.G., Moreira, M.R., Valle, C.E., and Roura, S.I., 2008. Preliminary characterization of bacteriocin like substances from lactic acid bacteria isolated from organic leafy vegetables. LWT - Food Science and Technology. 41(3): 432-441.

Somsiri, T., Oanh, D.T.H., Chinabut, S., Phuong, N.T., Shariff, M., Yusoff, F., Bartie, K., Giacomini, M., Robba, M., Bertone, S., Huys, G., and Teale, A., 2006. A simple device for sampling soft pond bottom sediment. Aquaculture. 258: 650-654.

Tambekar, D.H., Bhutada, S.A., Choudhary, S.D., and Khond, M.D., 2009. Assessment of potential probiotic bacteria isolated from milk of domestic animals. J Appl Biosci. 15: 815-819.

Tran Huu Loc, Nunan, L., Redman, R.M., Mohney, L.L., Pantoja, C.R., Fitzsimmons, K., and Lightner, D.V., 2013. Determination of the infectious nature of the agent of acute hepatopancreatic necrosis syndrome affecting penaeid shrimp. Diseases of aquatic organisms. 105: 45-55.

Tran Huu Loc, Fitzsimmons, K., and Lightner, D.V., 2014. The Acute Hepatopancreatic Necrosis Disease (AHPND/EMS) in shrimp: From the Academic Science Perspective to the Production Point of View. Aquaculture Asia Pacific Magazine.

Trinh Hung Cuong, 2011. Isolation of Lactobacillusspp. from tiger shrimp in intensive farming system could antagonize pathogenic Vibrio sp. Master thesis, Can Tho University, Vietnam.

Zorriehzahra, M.J. and Banaederakhshan, R., 2015. Early Mortality Syndrome (EMS) as new Emerging Threat in Shrimp Industry. Advances in Animal and Veterinary Sciences. 3: 64-72.

Most read articles by the same author(s)