Thi Hoang Oanh Dang * and Truong Quoc Phu

* Correspondence: Thi Hoang Oanh Dang (email: dthoanh@ctu.edu.vn)

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Abstract

This study was conducted to determine the effect of different salinities on striped catfish (Pangasianodon hypophthalmus) susceptibility to hemorrhagic disease causing by Aeromonas hydrophila bacteria. The experiment was set up in plastic tanks at various salinities (0, 3, 5, 8, 11 and 14‰). Experimental fish (about 25 ± 0.8g/fish) were challenged with A. hydrophila bacteria by intramuscular injection. After infection, the mortality and disease signs were recorded for 14 days. At the same time, bacteria re-isolation and re-identification were carried out with infected fish samples that displayed signs of lethargy, disorientation, hemorrhagic in anal, peritoneal sinus and spleen. Recorded cumulative mortality after 14 days was 86.7% at salinity 0‰ and 8.3% and 11.7% at salinities 11 and 14‰, respectively. At higher salinity, striped catfish is less susceptible to A. hydrophila bacteria than at lower salinities.

Keywords: Aeromonas hydrophila, hemorrhagic disease, Pangasianodon hypophthalmus, salinity

Article Details

References

Bartie, K., Oanh, D. T. H., Huys, G., Dickson, C., Cnockaert, M., Swings, J., Phương, N. T., & Teale, A. (2006). Application of Rep-PCR and Pulsed-field gel electrophoresis for typing chloramphenicol resistant bacterial isolates in a molecular epidemiology study of aquaculture sites in the Mekong Delta. Journal of Biotechnology, 4(1), 31-40.

Hoang, A. H., Xuan, T.T. T., Nga, L. P., & Oanh, D. T. H. (2019). Selection of phages to control Aeromonas hydrophila – An infectious agent in striped Catfish. Biocontrol Science, 24(1), 23-28, https://doi.org/10.4265/bio.24.23.

Oanh, D. T. H., & Hien, N. D. (2012). Isolation and determination of the ability to cause hemorrhagic disease on rice eel (Monopterus albus) of bacteria Aeromonas hydrophila. Can Tho University Journal of Science, 22c, 173-182 (in Vietnamese).

Thy, D. T. M., Dung, T. T., & Phuong, P. T. K. (2014). Histopathology of clown knife fish (Chitala chitala) infected with Aeromonas hydrophila bacteria. Can Tho University Journal of Science. Special issue on Aquaculture and Fisheries, 2, 29-36 (in Vietnamese).

Khang, N. K., Kotera, A., Sakamoto, T., & Yokozawa, M. (2008). Sensitivity of Salinity Intrusion to Sea Level Rise and River Flow Change in Vietnamese Mekong Delta-Impacts on Availability of Irrigation Water for Rice Cropping. Journal of Agricultural Meteorology, 64(3), 167-176.

Miyazaki, T., & Kaige, N. 1985. A histopathological study on motile aeromonad disease in Crucian carp. Fish Pathology, 21, 181-185.

Panangala, V. S., Shoemaker, C. A., Van Santen, V. L., Dybvig, K., Klesius, P. H. (2007). Multiplex-PCR for simultaneous detection of three bacteria fish pathogen, Flavobacterium columnare, Edwardsiella ictaluri and Aeromonas hydrophila. Disease of aquatic organisms, 74(3), 199-208, https://doi.org/10.3354/dao074199.

Phuc, T. H. N., Huong D. T. T., Mather, P. B., & Hurwood, D. A. (2014). Experimental assessment of the effects of sublethal salinities on growth performance and stress in cultured tra catfish (Pangasianodon hypophthalmus). Fish Physiology and Biochemistry, https://doi.org/10.1007/s10695-014-9972-1.

Reed, L. J., & Muench, H. (1938). A simple method of estimating fifty percent end points. America Journal of Hygiene, 27, 112-113.

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