Investigation of parasites in the digestive tract of white leg shrimp (Litopenaeus vannamei) cultured at coastal farms in the Mekong Delta
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Abstract
A total of 291 white leg shrimp samples were collected from 70 cultured ponds in Soc Trang, Bac Lieu and Ca Mau provinces in the Mekong Delta and subjected to endoparasitic detection in the digestive tract. Collected shrimps displayed unhealthy behaviors such as stop or less feeding and lethagic swimming. Pathological signs in the gastrointestinal tract include (1) empty midgut and stomach together with pale and atrophy hepatopancreas; (2) empty, little or discontinued food in the midgut; (3) slow growth and variation in sizes; and (4) white feces. The results from fresh and Giemsa stained smears methods revealed that 96.5% of sampled shrimps were infected by gregarine parasite at different developmental stages. A prevalence infection of 24.7% was recorded with Vermiform present in the hepatopancreas by fresh smear and histology. Histopathological analysis noted that 7.9% of collected shrimp samples had Enterocytozoon hepatopenaei (EHP) spores in hepatopancreas and midgut and confirmed by PCR analysis.
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References
Chakraborti, J., & Bandyopadhyay, P.K. (2010). First record of a parasitic septate gregarines (Apicomplexa: Sporozoea) in the shrimp Peneaus monodon in Sundarbans of West Bengal. Journal of Parasitic Diseases, 34(1), 40-43. https://doi.org/10.1007/s12639-010-0002-7.
Lightner, D.V. (1996). A handbook of shrimp pathology and diagnostic procedure for disease of shrimp. World Aquaculture Society.
Sriurairatana, S., Boonyawiwat, V., Gangnonngiw, W., Laosutthipong, C., Hiranchan, J. & Flegel, T.W. (2014). White feces syndrome of shrimp arises from transformation, sloughing and aggregation of hepatopancreatic microvilli into vermiform bodies superficially resembling gregarines. PloS One, 9(6), e99170, https://doi.org/10.1371/journal.pone.0099170.
Tang, K.F., Pantoja, C.R., Redman, R.M., Han, J.E., Tran, L.H., & Lightner, D.V. (2015). Development of in situ hybridization and PCR assays for the detection of Enterocytozoon hepatopenaei (EHP), a microsporidian parasite infecting penaeid shrimp. Journal of Invertebrate Pathology, 130, 37-41. https://doi.org/10.1016/j.jip.2015.06.009
Tang, K.F., Han, J.E., Aranguren, L.F., White-Noble, B., Schmidt, M.M., Piamsomboon, P., Risdiana, E. & Hanggono, B. (2016). Dense populations of the microsporidian Enterocytozoon hepatopenaei (EHP) in feces of Penaeus vannamei exhibiting white feces syndrome and pathways of their transmission to healthy shrimp. Journal of Invertebrate Pathology, 140, 1-7. https://doi.org/10.1016/j.jip.2016.08.004.
Tangprasittipap, A., Srisala, J., Chouwdee, S., Somboon, M., Chuchird, N., Limsuwan, C., Srisuvan, T., Flegel, T.W. & Sritunyalucksana, K. (2013). The microsporidian Enterocytozoon hepatopenaei is not the cause of white feces syndrome in whiteleg shrimp Penaeus (Litopenaeus) vannamei. BMC Veterinary Research, 9, 139. https://doi.org/10.1186/1746-6148-9-139
Tourtip, S., Wongtripop, S., Stentiford, G.D., Bateman, K.S., Sriurairatana, S., Chavadej, J., Sritunyalucksana, K., & Withyachumnarnkul, B. (2009). Enterocytozoon hepatopenaei sp. nov. (Microsporida: Enterocytozoonidae), a parasite of the black tiger shrimp Penaeus monodon (Decapoda: Penaeidae): Fine structure and phylogenetic relationships. Journal of Invertebrate Pathology, 102(1), 21–29. https://doi.org/10.1016/j.jip.2009.06.004.
Tuntiwaranuruk, C., Boonmameepool, J., Noppharatarphakul. J., & Upatham, E. S. (2015). Ultrastructure of Gametocyst of Parasitic Protozoan, Nematopsis sp. in Black Tiger Shrimp Penaeus monodon from the Gulf of Thailand. Acta Protozoologica, 54(2), 137–142. https://doi.org/10.4467/16890027AP.15.011.2736