Disease-reducing effects of antagonistic soil bacteria on Fusarium basal rot of shallot caused by Fusarium oxysporum in Vĩnh Châu, Sóc Trăng

Nguyen Dac Khoa , Tran Viet Quyen * and Chau Hoang Trung Tin

* Corresponding author (vietquyen.danny@gmail.com)

Article Sidebar

Full Text: PDF

Received: 10 Jun 2016
Revised: 18 Sep 2016
Accepted: 29 Jul 2017
Published: 31 Jul 2017
DOI: 10.22144/ctu.jen.2017.024
Views
318
Downloads
131
Crossref
Scopus
Google Scholar
Europe PMC
How to Cite
Nguyen, D. K., Tran, V. Q., & Chau, H. T. T. (2017). Disease-reducing effects of antagonistic soil bacteria on Fusarium basal rot of shallot caused by Fusarium oxysporum in Vĩnh Châu, Sóc Trăng. CTU Journal of Innovation and Sustainable Development , (06), 31-37. https://doi.org/10.22144/ctu.jen.2017.024
Issue
No. 06 (2017)

Main Article Content

Abstract

Fusarium basal rot caused by Fusarium oxysporum is one of the most important diseases of shallot (Allium cepa var. ascalonicum). This study aims at isolating soil bacteria from Vinh Chau (Soc Trang) and testing for their antagonistic and disease-reducing effects which could be used for biological control of the disease. A number of 224 isolates were collected and tested for their antagonistic effects on mycelial growth and conidia germination of the pathogen. Among those, three isolates exhibited the strongest effects. These isolates were tested for their disease-reducing effects under greenhouse conditions using two application methods, i.e., bulb coating and soil drenching. Two treatments using ATB-24 and ATA-33 (108 CFU/mL) showed the highest effects on disease incidence and severity; their effects remained through all assessment time points (until 65 days after planting). The results suggest the applicability of the ATB-24 and ATA-33 isolates as biological control agents for Fusarium basal rot of shallot.
Keywords: Allium cepa var. ascalonicum, antagonistic bacteria, Fusarium basal rot, Fusarium oxysporum, shallot

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

References

Cramer, C.S., 2000. Breeding and genetics of Fusarium basal rot resistance in onion. Euphityca. 115:159-166.

Đặng Thị Cúc, 2008. Effects of microorganisms-organic fertilizers on ameliorating soil fertility and enhancing disease-resistance in shallots in Vĩnh Châu. MSc thesis. Can Tho University. Cần Thơ, Vietnam (in Vietnamese).

Đặng Thị Cúc, 2011. Shallot cultivation, accessed on 10 May 2015. Available from http://danviet.vn/nha-nong/ky-thuat-trong-hanh-tim-xuat-khau-61891.html (in Vietnamese).

Défago, G., Haas, D., 1990. Pseudomonads as antagonists of soilborne plant pathogens: modes of action and genetic analysis. In: Bollag, J.M., Stotsky, G. (Eds.). Soil Biochemistry. Marcel Dekker Inc. New York, pp. 249-291.

Dhanasekaran, D., Thajuddin, N., Panneerselvam, A., 2012. Applications of Actinobacterial Fungicides in Agriculture and Medicine. In: Dhanasekaran, D., Thajuddin, N., Panneerselvam, A. (Eds). Fungicides for Plant and Animal Diseases. Intech. Rijeka, pp. 1-27.

Hammerschmidt, R., 2007. Introduction: Definitions and some history. In: Walters D., Newton A. and Lyon G. (Eds). Induced resistance for plant defence: a sustainable approach to crop protection. Backwell Publishing. USA, pp 1-8.

Javandira, C., Luqman, Q.A., Arifin, N.S., Abdul, L.A., 2013. The potency of Bacillus sp. and Pseudomonas sp. as biological control agents against corn leaf blight disease caused by Pantoea sp. Agrivita. 35: 103-109.

Kim, W.G., Weon, H.Y., Seok, S.J., Lee, K.H., 2008. In vitro antagonistic characteristics of Bacilli isolates against Trichoderma spp. and three species of mushrooms. Mycobiology. 36: 266-269.

Moulin, F., Lemanceau, P., Alabouvette, C., 1994. Control by fluorescent pseudomonads of Pythium aphanidermatum root rot responsible for yield reduction in soilless culture of cucumber. In: Ryder, M.H., Stephens, P.M. and Bowen, G.D. (Eds.). Improving Plant Productivity with Rhizosphere Bacteria. CSIRO Publishing. Adelaide, pp. 47-50.

Nguyễn Đắc Khoa, Dương Minh, Phạm Văn Kim, 2010. Bio-control products for sustainable and environmentally friendly management of diseases of rice, fruit trees, and vegetables. Can Tho University Journal of Science. 16: 117-126 (in Vietnamese).

Nguyễn Thị Nguyệt, 2014. Management of diseases and insect pests of shallots, accessed on 18 June 2015. Available from http://dost-bentre.gov.vn/TinTuc/NoiDung.aspx?tintuc=6260 (in Vietnamese).

Prapagdee, B., Kuekulvong, C., Mongkolsuk, S., 2008. Antifungal Potential of Extracellular Metabolites Produced by Streptomyces hygroscopicus against Phytopathogenic Fungi. International Journal of Biological Sciences. 4: 330-337.

Quách Nhị, 2009. Shallot cultivation in Vĩnh Châu, accessed on 17 June 2015. Available from http://www.soctrang.gov.vn/wps/portal/!ut/p/c4/04_SB8K8xLLM9MSSzPy8xBz9CP0os3gLR1dvZ09LYwOL4GAnA08TRwsfvxBDR2MvE_2CbEdFAJyB5MU!/?WCM_GLOBAL_CONTEXT=/wps/wcm/connect/web+content/soctrangsite/sanxuatthitruong/sanphamdactrung/vinhchaumuahanhtim (in Vietnamese).

Rodríguez-Algaba, J., Sørensen, J.C., Sørensen, H., Khoa, N.Đ., Collinge, D.B., Jørgensen, H.J.L., 2015. Activity-guided separation of Chromolaena odorata leaf extract reveals fractions with rice disease-reducing properties. European Journal of Plant Pathology. 143: 331-341.

Scher, F.M., 1986. Biological control of Fusarium wilts by Pseudomonas putida and its enhancement by EDDHA. In: Swinburne, T.R. (Ed.). Iron, Siderophores and Plant Diseases. Plenum Press. New York, pp. 109-117.

Sigee, D.C., 2005. Bacterial Plant Pathology: Cell and Molecular Aspects. Cambridge University Press. Cambridge, pp. 276-278.

Sintayehu, A., Ahmed, S., Fininsa, C., Sakhuja, P.K., 2014. Evaluation of Green Manure Amendments for the Management of Fusarium Basal Rot (Fusarium oxysporum f.sp. cepae) on Shallot. International Journal of Agronomy. 2014: 1-6.

Stankovic, S., Levic, J., Petrovic, T., Logrieco, A., Moretti, A., 2007. Pathogenicity and mycotoxin production Fusarium proliferatum isolated from onion and garlic in Serbia. European Journal of Plant Pathology. 118: 165-172.

Tashiro, Y., Miyazaki, S., Kanazawa, K., Hashimoto, H., 1983. Cytogenetic Studies on the Origin of Allium wakegi Araki: III. Restoration of the fertility of A. wakegi by doubling the chromosome complement. Bulletin of the faculty of agriculture Saga University. 55: 125-129.

Thrane, C., Nielsen, M.N., Sørensen, J., Olsson, S., 2001. Pseudomonas fluorescens DR54 reduces sclerotia formation, biomass development, and disease incidence of Rhizoctonia solani causing damping-off in sugar beet. Microbial Ecology. 42: 438-445.

Todorova, S., Kozhuharova, L., 2010. Characteristics and antimicrobial activity of Bacillus subtilis strains isolated from soil. World Journal of Microbiology and Biotechnology. 26: 1207-1216.