Nguyen Hoang Minh Sang * , Nguyen Dac Khoa and Chau Hoang Trung Tin

* Correspondence: Nguyen Hoang Minh Sang (email:

Main Article Content


This study aims at identifying the pathogen causing sweet potato wilt in Binh Tan district, Vinh Long province of Vietnam using morphological characterization, Koch’s postulates and sequencing the ITS region amplified by primer set ITS1/ITS4. A total of seven fungal isolates were obtained from 14 infected stem samples collected from Binh Tan district. Based on morphological characterization, two isolates CD3 and CD5 were classified into the Fusarium genus, one of the major plant pathogen genera causing wilt diseases. CD5 exhibited typical wilt symptoms using Koch’s postulates and was identified as Fusarium solani based on its ITS sequence. This result serves as a basis for further studies on disease management.
Keywords: Fusarium solani, pathogen identification, sweet potato, wilt

Article Details


Brodeur, J., 2012. Host specificity in biological control: Iinsights from opportunistic pathogens. Evolutionary Applications. 5(5): 470–480.

Burgess, L.W., Knight, T.E., Tesoriero, L., and Phan, T.H., 2008. Diagnostic manual for plant diseases in Vietnam (No. LC-0362). Australian Centre for International Agricultural Research. Canberra, 210 pages.

Campbell, C.K., and Johnson, E.M., 2013. Identification of pathogenic fungi. Health Protection Agency. London, 337 pages.

Clark, C.A., Holmes, G.J., and Ferrin, D.M., 2009. Major Fungal and Bacterial Diseases. In: Loebenstein, G., Thottappilly, G., (Editors). The sweetpotato. Springer. Netherlands, pp. 81-103.

Collins, W.W., and Nielsen, L.W., 1976. Fusarium wilt resistance in sweetpotatoes. Phytopathology. 66: 489-493.

FAOSTAT (The Food and Agriculture Organization Corporate Statistical Database), 2014. FAO Statistical Databases, accessed on 05 February 2017. Available from

Gaddeyya, G., Niharika, P.S., Bharathi, P., and Kumar, P.R., 2012. Isolation and identification of soil mycoflora in different crop fields at Salur Mandal. Advances in Applied Science Research. 3(4): 2020-2026.

Gardes, M., and Bruns, T.D., 1993. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Molecular ecology. 2(2): 113-118.

Hoc, N.T., 2016. Isolation and identification of shallot (Allium ascalonicum) pathogens in Vinh Chau, Soc Trang. MSc thesis. Can Tho University. Can Tho , Cần Thơ (in Vietnamese).

Khoa, N.D., Giau, N.D.N., and Tuan, T.Q., 2016. Effects of Serratia nematodiphila CT-78 on rice bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae. Biological Control. 103: 1-10.

Le Thi Thanh Hien, Le Vinh Thuc, Nguyen Thi Thanh Thuy, and Nguyen Bao Ve, 2014. Effects of calcium fertilizer dosage on the growth, yield and quality of sweet potato (Ipomoea batatas Lam.) at Binh Tan District, Vinh Long Province. Can Tho University Journal of Science. 4: 24-31 (in Vietnamese).

Lemanceau, P., and Alabouvette, C., 1991. Biological control of Fusarium diseases by fluorescent Pseudomonas and non-pathogenic Fusarium. Crop Protection. 10(4): 279-286.

Moyer, J.W., Campbell, C.L., and and Averre, C.W., 1982. Stem canker of sweet potato induced by Fusarium solani. Plant Disease. 66: 65-66.

Ngo, T.X., and Loc, D.T., 2005. Root and tuber crops and intensive farming techniques - Volume 1: Sweet potatoes. Labour and Social Publisher Company Limited. Ha Noi, 103 pages (in Vietnamese).

Quyen, T.V., Tin, C.H.T. and Khoa, N.D., 2016. Disease-reducing effects of antagonistic soil bacteria on Fusarium basal rot of shallot caused by Fusarium oxysporum in Viĩnh Chau, Soc Trang. Can Tho University Journal of Science. 6: 31-37.

Son, L., 2016. Sweet potatoes - A potential for food processing, accessed on 25 June 2016. Available from: (in Vietnamese).

Vu, D.H., Loc, D.T., Ho, T.V., and Kim., H., 1989. The Sweet potato in Vietnam. In: International Potato Center (Editor). Improvement of Sweet Potato (Ipomoea batatas) in Asia. Workshop on Sweet Potato Improvement in Asia, 24-28 October 1988, ICAR, India. International Potato Center. Peru, pp. 105-107.

White, T.J., Bruns, T., Lee, S., and Taylor, J., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J., and White, T.J. (Eds.). PCR Protocols: A guide to methods and applications. Academic Press. San Diego, pp. 315-322.