Selection of carrier materials for formulation of the antagonistic Bacillus spp. against rice bacterial leaf blight

Dang Hoai An * , Nguyen Dac Khoa and Le Nguyen Xuan Thanh

* Corresponding author (danghoaianvl@gmail.com)

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Received: 22 May 2019
Revised: 16 Aug 2019
Accepted: 29 Nov 2019
Published: 30 Nov 2019
DOI: 10.22144/ctu.jen.2019.034
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Dang, H. A., Nguyen, D. K., & Le, N. X. T. (2019). Selection of carrier materials for formulation of the antagonistic Bacillus spp. against rice bacterial leaf blight. CTU Journal of Innovation and Sustainable Development , 11(3), 19-27. https://doi.org/10.22144/ctu.jen.2019.034
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Vol. 11 No. 3 (2019)

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Abstract

Bacillus pumilus ST-115, B. safensis AG-131, B. stratosphericus AG-62 and B. subtilis TG-71 showed strong antagonistic effects against Xanthomonas oryzae pv. oryzae causing rice bacterial leaf blight. This research aims at evaluating the effects of carrier materials on cell densities, the antagonistic effects and disease-reducing effects of these four bacterial strains and selecting the suitable carrier material(s) for each of them. Five carrier materials, i.e., talc powder, rice bran, rice husk powder, rice grain powder and rice kernel powder, were used to store the Bacillus spp. in a six-month period at room temperature. Results show that after six-month storing, formulations of Bacillus spp. using talc powder, rice bran and rice husk powder could remain the cell densities at over 106 CFU/g formulation, the antagonistic effects on agar plates and the disease-reducing effects under greenhouse conditions. Rice bran was the suitable carrier material for B. safensis AG-131, rice husk powder for B. pumilus ST-115 and talc powder for both B. stratosphericus AG-62 and B. subtilis TG-71.
Keywords: antagonistic bacteria, Bacillus, bacterial leaf blight, carrier material, formulation

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