Identification of shallot pathogens in Vĩnh Châu town of Sóc Trăng province

Bui Thanh Thu * , Nguyen Dac Khoa and Tran Quoc Tuan

* Corresponding author (buithanhthu27@gmail.com)

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Received: 10 May 2019
Revised: 04 Jul 2019
Accepted: 29 Nov 2019
Published: 30 Nov 2019
DOI: 10.22144/ctu.jen.2019.033
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Bui, T. T., Nguyen, D. K., & Tran, Q. T. (2019). Identification of shallot pathogens in Vĩnh Châu town of Sóc Trăng province. CTU Journal of Innovation and Sustainable Development , 11(3), 11-18. https://doi.org/10.22144/ctu.jen.2019.033
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Vol. 11 No. 3 (2019)

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Abstract

Shallot (Allium ascalonicum) is an important crop of Vinh Chau town, Soc Trang province of Vietnam. This study aims at identifying the contemporary pathogens in shallot fields in this region. The identification was done using the Koch’s postulates, morphological observation and molecular techniques. A collection of 124 infected shallot samples was obtained from three cropping seasons during 2015-2016 at three major shallot producing areas of Vinh Chau town. From these samples, a total of 49 bacterial and 118 fungal isolates were obtained. Using the Koch’s postulates, 160 isolates were confirmed to be shallot pathogens. Based on morphological observation and molecular techniques, i.e., PCR using specific primers and sequencing of the 16S rRNA genes, the pathogens were identified as Erwinia carotovora (soft rot), Pseudomonas aeruginosa (bulb rot), Aspergillus niger (black mold), Colletotrichum gloeosporioides (anthracnose) and Fusarium oxysporum (basal rot). Among these, E. carotovora and F. oxysporum appeared to be the predominant pathogens causing bulb rot in the shallot fields of Vinh Chau town.
Keywords: Aspergillus niger, Colletotrichum gloeosporioides, Erwinia carotovora, Fusarium oxysporum, Pseudomonas aeruginosa, shallot

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References

Alberto, R.T., 2014. Pathological response and biochemical changes in Allium cepa L. (bulb onions) infected with anthracnose-twister disease. Plant Pathology and Quarantine. 4(1): 23–31.

Bayraktar, H. and Dolar, F.S., 2011. Molecular identification and genetic diversity of Fusarium species associated with onion fields in Turkey. Journal of Phytopathology. 159(1): 28-34.

Benson, H.J., 2002. Microbiological applications: A laboratory manual in general microbiology. McGraw-Hill. New York, 496 pages.

Burgess, L.W., Knight, T.E., Tesoriero, L. and Phan, H.T., 2008. Diagnostic manual for plant diseases in Viet Nam. ACIAR Monograph 129. Australian Centre for International Agricultural Research. Canberra, 210 pages.

Campbell, C.K., Johnson, E.M. and Warnock D.W., 2013. Identification of Pathogenic Fungi, Second Edition. Wiley-Backwell. UK, 350 pages.

Conn, K.E., Lutton, J.S., Rosenberger, S.A., et al., 2012. Onion disease guide: Aa practical guide for seedmen growers and agricultural advisors. Seminis Vegetable Seeds. USA, 72 pages.

Cramer, C.S., 2000. Breeding and genetics of Fusarium basal rot resistance in onion. Euphytica. 115(3): 159-166.

Dang Thi Cuc, 2008. Effects of microbial organic fertilizer on improving soil nutritions and disease resistance of shallot (Allium cepa) in Vinh Chau town. Master thesis. Can Tho University. Can Tho city (in Vietnamese).

Dang Thi Cuc, 2014. Shallot cultivation for exportation. Plant Protection Department of Soc Trang Province. Soc Trang, 42 pages (in Vietnamese).

Darrasse, A., Priou, S., Kotoujansky, A. and Bertheau, Y., 1994. PCR and restriction fragment length polymorphism of a pel gene as a tool to identify Erwinia carotovora in relation to potato diseases. Applied and Environmental Microbiology. 60(5): 1437-1443.

Dinakaran, D., Gajendran, G., Mohankumar, S. et al., 2013. Evaluation of integrated pest and disease management module for shallots in Tamil Nadu, India: A Farmer’s participatory approach. Journal of Integrated Pest Management. 4(2): B1-B9.

Dutta, B., Gitaitis, R.D., Webster, T.M., Sanders, H., Smith, S. and Langston, D.B., 2014. Distribution and survival of Pseudomonas sp. on Italian ryegrass and curly dock in Georgia. Plant Diseases. 98(5): 660-666.

Kamle, M., Pandey, B.K., Kumar, P. and Muthu, K.M., 2013. A species-specific PCR based assay for rapid detection of mango anthracnose pathogen Colletotrichum gloeosporioides Penz. and Sacc. Journal of Plant Pathology and Microbiology. 4(6):184-188.

Lan, W.W., Nishiwaki, Y., Akino S. and Kondo N., 2013. Soft rot of root chicory in Hokkaido and its causal bacteria. Journal of General Plant Pathology. 79(3): 182-193.

Le Hoang Le Thuy and Pham Van Kim, 2008. Classification to species of Colletotrichum isolates, causal agent of anthracnose disease on mango and durian in the Mekong Delta and test for effectiveness of six fungicides to the fungal species. Can Tho University Journal of Science. 10: 31- 40 (in Vietnamese).

Melchers, J.G.W., Verweij, E.P., Hurk, D.V.P. et al., 1994. General primer-mediated PCR for detection of Aspergillus species. Journal of Clinical Microbiology. 32(7): 1710-1717.

Mishra, K.P., Fox, T.V.R. and Culham, A., 2003. Development of a PCR-based assay for rapid and reliable identification of pathogenic Fusaria. FEMS Microbiology Letters. 218: 329-332.

Mishra, R.K., Jaiswal, R.K., Kumar, D., Saabale, P.R. and Singh, A., 2014. Management of major diseases and insect pests of onion and garlic: A comprehensive review. Journal of Plant Breeding and Crop Science. 6(11): 160-170.

Nguyen Duc Thang, 1999. Survey of cultivation and storage conditions and preliminary testing for the effects of some agro-chemicals on shallot under storage conditions (Allium cepa group aggregatum) in Soc Trang. Master thesis. Can Tho University. Can Tho city (in Vietnamese).

Nova, V.M.X., Borges, L.R., de Sousa, A.C.B. et al., 2011. Pathogenicity for onion and genetic diversity of isolates of the pathogenic fungus Colletotrichum gloeosporioides (Phyllachoraceae) from the State of Pernambuco, Brazil. Genetics and Molecular Research. 10(1): 311-320.

Prithiviraj, B., Vikram, A., Kushalappa, C.A. and Yaylayan, V., 2004. Volatile metabolite profiling for the discrimination of onion bulbs infected by Erwinia carotovora ssp. carotovora, Fusarium oxysporum and Botrytis alli. European Journal of Plant Pathology. 110(4): 371-377.

Schwartz, H.F. and Bartolo, M.E., 1995. Soil-borne diseases of onion. Colorado State University Cooperative Extension. Colorado, 45 pages.

Shehu, K. and Muhammad, S., 2011. Fungi associated with storage rots of onion bulbs in Sokoto, Nigeria. International Journal of Modern Botany. 1(1): 1-3.

Shivaji, S., Chaturvedi, P., Suresh, K. et al., 2006. Bacillus aerius sp. nov., Bacillus aerophilus sp. nov., Bacillus stratosphericus sp. nov. and Bacillus altitudinis sp. nov., isolated from cryogenic tubes used for collecting air samples from high altitudes. International Journal of Systematic and Evolutionary Microbiology. 56(7): 1465-1473.

Sintayehu, A., Ahmed, S., Fininsa, C. and Sakhuja, P.K., 2014. Evalution of green manure amendments for the management of Fusarium basal rot on shallot. International Journal of Agronomy. 2014(36): 1-6.

Stankovic, S., Levic, J., Petrovic, T., Logrieco, A. and Moretti, A., 2007. Pathogenicity and mycotoxin production by Fusarium proliferatum isolated from onion and garlic in Serbia. The European Journal of Plant Pathology. 118(2): 165-172.

Suhardi, H.A., 1993. Anthracnose on shallot (Allium cepa group aggregatum) in Java. Onion Newsletter for the Tropics. 5: 48-50.

Taylor, A., Vágány, V., Jackson, A.C., Harrison, R.J., Rainoni, A. and Clarkson, J.P., 2016. Identification of pathogenicity-related genes in Fusarium oxysporum f. sp. cepae. Molecular Plant Pathology. 17(7): 1032-1047.

Vo Hoang Nghiem, 2012. Survey on shallot diseases (Allium ascalonicum L.) in seed production season 2012 in Vinh Châu town, Soc Trang province. Bachelor thesis. Can Tho University. Can Tho city (in Vietnamese).

Vu Trieu Man, 2007. Plant pathology. Agriculture Publishing House. Ha Noi, 233 pages (in Vietnamese).

Weisburg, W.G., Barns, S.M., Pelletier, D.A. and Jane, D.J., 1991. 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology. 173(2): 697–703.

Wright, J.P., Clark, G.R. and Hale, N.C., 1993. A storage soft rot of New Zealand onions caused by Pseudomonas gladioli pv. alliicola. New Zealand Journal of Crop and Horticultural Science. 21(3): 225-227.

Zakham, F., Bazoui, H., Akrim, M. et al., 2011. Evaluation of conventional molecular diagnosis of Mycobacterium tuberculosis in clinical specimens from Morocco. The Journal of Infection in Developing Countries. 6(1): 40-45.

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