Nguyen Thi Kim Ha * , Nguyen Tinh Em , Nguyen Minh Ngoc , Yasuaki Takagi , Nguyen Thanh Phuong and Do Thi Thanh Huong

* Corresponding authorNguyen Thi Kim Ha

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The present study was to investigate the effects of different salinities (0 (control), 3, 6, 9, 12, 15‰) in striped catfish (Pangasianodon hypophthalmus) at larvae stage. Four-hundred individuals were randomly distributed to each 500-L composite tank (containing 250-L water) in triplicates. After 60 days, fish was sampled to determine survival rate, growth performance, hematological parameters, stress response indicators, and enzymatic activities. The results showed that the survival rate was highest in 6‰ (60.9%) and lowest in 15‰ (1.25%) (p<0.05). Fish exposed to salinities from 0 to  9‰ showed an increasing trend in growth. The osmolality of fish reared in 9 and 12‰ was  significantly higher than that in  the lower salinities (p<0.05). Similarly, concentrations of Na+, K+, and Cl- increased remarkably at high salinities. Chymotrypsin and trypsin activities were reduced by increased salinities (p<0.05), which were lowest at 15‰ (69.2±2.46 U/min/mg protein and 1.46±0.01 mU/min/mg protein, respectively). Stress indicators such as glucose and cortisol concentrations were not significantly depended on the salinity in the range of 0 to 12‰. Striped catfish larvae showed an adaptable response to high salinity and the appropriate salinity range for larvae rearing was below 6‰.

Keywords: Growth, physiological parameters, salinity, striped catfish

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Abdel-Tawwab, M., Mousa, M. A., Sharaf, S. M. & Ahmad, M. H. (2005). Effect of crowding stress on some physiological functions of Nile tilapia, Oreochromis niloticus (L.) fed different dietary protein levels. International Journal of Zoological Research1(1), 41-47.

Al-Hilali, H. A. & Al-Khshali, M. S. (2016). Effect of water salinity on some blood parameters of common carp (Cyprinus carpio). International Journal of Applied Agricultural Sciences, 2(1), 17-20.

Ane, A., Taher, T., Islam, S. M. M., Uddin, H., Islam, M., & Shahjahan, M. (2019). Striped catfish (Pangasianodon hypophthalmus) could be suitable for coastal aquaculture. Journal of Applied Ichthyology, 35(4), 994-1003.

Barman, U.K., Jana, S.N., Garg, S.K., Bhatnagar, A., & Arasu, A.R.T. (2005). Effect of inland water salinity on growth, feed conversion efficiency, and intestinal enzyme activity in growing grey mullet, Mugil cephalus (Linn.): Field and laboratory studies. Aquaculture International: Journal of the European Aquaculture Society, 13(3), 241-256 Doi: 10.1007/s10499-004-2479-5.

Bernfeld, P. (1951). Enzymes of starch degradation and synthesis. In. F. F. Nord (Eds.), Advances in enzymology and related areas of molecular biology (pp. 379-428). Interscience Publishers, Inc. Doi: 10.1002/9780470122570.ch7.

Bœuf, G., and Payan, P. (2001). How should salinity influence fish growth? Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 130, 411–423.

Boyd, C. E. (1998). Water quality for ponds Aquaculture. Research and Development series No.43, August 1998, Alabama, 37 pp.

Chen, P. (1998). Effects of salinity on digestive enzyme activity of Pagrosomus major young fish. Journal-Xiamen University Natural Science, 37, 756-761. (English Abstract).

Evans, D. H. (1993). Osmotic and Ionic Regulation. In D. H. Evans (Eds.), The Physiology of Fishes (pp. 315-341). CRC Press, Boca Raton.

Giang, T.T. (2008). Effects of salinity on physiological respond and growth performance of mudskipper (Pseudapocryptes lanceolatus, Bloch 1801) (master’s thesis). Can Tho University, Can Tho city, Vietnam (in Vietnamese).

Hamed, S. S., Jiddawi, N. S., & Poj, B. (2016). Effect of salinity levels on growth, feed utilization, body composition, and digestive enzymes activities of juvenile silver pompano Trachinotus blochii. International Journal of Fisheries and Aquatic Studies, 4, 279–283.

Hoa, H. T. (2014). Effects of salinity on digestive enzyme activities of striped catfish (Pangasianodon hypophthalmus) (master’s thesis). Can Tho University, Can Tho city, Vietnam (in Vietnamese).

Hosseini, P., Vahabzadeh Roodsari, H., Sayyad Bourani, M., & Kazemi, R. (2011). The effect of salinity stress on hematocrite and hemoglobin in fingerling Rainbow trout (Onchorhynchus mykiss). International Conference on Medical, Biological and Pharmaceutical Sciences, (ICMBPS'2011) Pattaya.

Hugget, A. S. G. and Nixon, D. A. (1957). Use of glucose oxidase, peroxidase and o-dianisidine in determination of blood and urinary glucose. The Lancet, 270(6991), 368-370. doi: 10.1016/s0140-6736(57)92595-3

Huong, D. T .T., Ky, T. M., Ha, N. T. K., Em, N. T., Takagi Y. & Phuong, N. T. (2020). Effects of salinity on physiological parameters, digestive enzyme activities, and growth of snakehead fish (Channa striata). Can Tho University Journal of Science. Special issue on Aquaculture and Fisheries, 56(1), 11-19. (in Vietnamese).

Huong, D. T. T. & Quyen, T. N. T. (2012). The effect of salinity on the embryonic development and osmoregulatory of the striped catfish (Pangasianodon hypophthalmus) larvae and fingerling stages. Can Tho University Journal of Science, 21b, 29-37 (in Vietnamese).

Huong, D. T. T. & Tu, N. V. (2010). Introduction to the physiology and fish and crustacean. Agricultural publishing house. Ho Chi Minh city (in Vietnamese).

Huong, D. T. T., Phuong, N. T., Wang, T., Thuy, N. H., & Bayley, M. (2008). Osmotic ang ionic regulation, food intake and growth of striped catfish (Pangasianodon hypophthalmus) exposed to different salinities. Handbook and abstract of the international symposium on catfish aquaculture in Asia: Present status and challenge for suitable development. Can Tho University. 5-6th December 2008. p.65.

Lam, N. C., Huong, D. T. T., Son, V. N., & Phuong, N. T. (2011). Physiological changes and growth of tra catfish (Pangasianodon hypophthalmus) exposed to different salinities. Can Tho University Journal of Science, 17a, 60-69. (in Vietnamese).

Lisboa, V., Barcarolli, I.F., Sampaio, L.A. & Bianchini, A. (2015). Effect of salinity on survival, growth and biochemical parameters in juvenile Lebranch mullet Mugil liza (Perciformes: Mugilidae). Neotropical Ichthyology13(2), 447-452.

Martinez-Alvarez, R.M., Hidalgo, M.C., Domezain, A., Morales, A.E., García-Gallego, M. & Sanz, A. (2002). Physiological changes of sturgeon Acipenser naccarii caused by increasing environmental salinity. Journal of experimental biology205(23), 3699-3706.

Martínez-Porchas, M., Martínez-Córdova, L.R. & Ramos-Enríquez, R. (2009). Cortisol and glucose: Reliable indicators of fish stress?. Pan-American Journal of Aquatic Sciences, 4, 158-178.

Molnar, C. & Gair, J. (2013). Osmoregulation and Osmotic Balance. Concepts of Biology-1st Canadian Edition.

MoNRE (Ministry of Natural Resources and Environment) (2016). Climate change and sea-level rise scenarios for Vietnam: Summary for policymakers.

Nahar, F., Haque, W., Ahsan, D.A., & Mustafa, M. D. G.  (2015). Effects of salinity changes on growth performance and survival of Climbing perch, Anabas testudineus (Bloch, 1795). Dhaka University Journal of Biological Science, 25(1), 65‐73.

Nam, P.T. & Huong, D.T.T. (2011). The osmoregulation and growth of hybrid yellow catfish juvenile (Clarias macrocephalus Gunther x Clarias gariepinus) exposed to the different salinities. Can Tho University Journal of Science, 20b, 39-47 (in Vietnamese).

Nguyen, A.L., Dang, V.H., Bosma, R.H., Verreth, J.A., Leemans, R. & De Silva, S.S. (2014). Simulated impacts of climate change on current farming locations of striped catfish (Pangasianodon hypophthalmus; Sauvage) in the Mekong Delta, Vietnam. Ambio. 43(8), 1059-1068.

Oser, B.L., (1965). Blood analysis. Hawk's physiological chemistry. p.1096.

Phuc, N. T. H., Huong, D. T. T., Mather, P. B., & Hurwood, D. A. (2014). Experimental assessment of the effects of sublethal salinities on growth performance and stress in 126 cultured tra catfish (Pangasianodon hypophthalmus). Fish Physiology and Biochemistry, 40, 1839-1848.

Pujante, I. M., Moyano, F. J., Martos-Sitcha, J. A., Mancera, J. M., & Martinez-Rodriguez, G. (2018). Effect of different salinities on gene expression and activity of digestive enzymes in the thick-lipped grey mullet (Chelon labrosus). Fish Physiological and Biochemistry, 44, 349–373.

Quyen, T. N. T. (2011). The effect of salinity on embryo development and osmoregulation of striped catfish (Pangasianodon hypophthalmus) (master’s thesis). Can Tho University, Can Tho city, Vietnam (in Vietnamese).

Smith, S. L. (1982). Introduction to Fish Physiology. T. F. H. Publications, Inc.

Thao, N. L. (2013). Effect of salinity on growth performance and stress of striped catfish (Pangasianodon hypophthalmus). Master’s thesis. Can Tho University, Can Tho city, Vietnam (in Vietnamese).

Tinh, N. T. (2013). Effects of salinity on digestive enzyme activities and growth performance of striped snakehead (Channa striata) (master’s thesis). Can Tho University, Can Tho city, Vietnam (in Vietnamese).

Tseng, H. C., Grendell, J. H. & Rothman, S. S. (1982). Food, duodenal extracts, and enzyme secretion by the pancrease. American Journal of Physiology, 243, 304–312.

Tsuzuki, M. Y., Sugai, J. K., Maciel, J. C., Francisco, C. J., & Cerqueira, V. R. (2007). Survival, growth and digestive enzyme activity of juveniles of the fat snook (Centropomus parallelus) reared at different salinities. Aquaculture, 271, 319–325.

Tuan, D. (1981). Fish physiology. Hanoi Agricultural Publishing House (in Vietnamese).

Usher, M. L., Talbot, C., & Eddy, F. B. (1990). Effects of transfer to seawater on digestion and gut function in Atlantic salmon smolts (Salmo salar L.). Aquaculture, 90, 85–96.

Varsamos, S., Nebel, C. & Charmantier, G. (2005). Ontogeny of osmoregulation in postembryonic fish: a review. Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, 141(4), 401-429.

VASEP, (2020). Total fisheries production in November 2020 reached 7.7 million tons, increased 1,7%. (access on Mar. 23, 2021).

Worthington T. M. (1982). Enzymes and Related Biochemicals. Biochemical Products Division, Worthington Diagnostic System, Freehold, NJ, USA.

Yin, F., Peng, S. M., Sun, P. & Shi, Z. H. (2010). Effects of low salinity on digestive enzyme activity in intestinal tract of juvenile silver pomfret Pampus argenteus. Journal of Marine Fisheries, 2. (English Abstract).

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