Pham Ngoc Nhu , Bui Thi Bich Hang , Nguyen Thanh Phuong , Patrick Kestemont and Do Thi Thanh Huong *

* Correspondence: Do Thi Thanh Huong (email:

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Effects of guava (Psidium guajava L.) and bhumi amla (Phyllanthus amarus Chum et Thonn) on haematology and thermal stress mitigation of striped catfish (Pangasianodon hypophthalmus) were investigated. In a 42-day trial, fish were administered 4 diets as control (without extract), 0.2% P. guajava (Pg0.2), 0.5% P. amarus (Pa0.5), and a mixture of Pg0.2 and Pa0.5 (Mix). Fish were then subjected to temperatures of 27°C, 31°C, and 35°C for another 42 days. Haematological parameters were highest at 35°C, but these parameters were not significantly different from values recorded at 31°C on day 14 post-temperature challenge. The Pg0.2 diet modified red blood cells, haematocrit, and haemoglobin (p<0.05). The lowest glucose concentration was recorded in Pg0.2 (57.4±1.34 mg/100 mL) and Mix (58.9±1.87 mg/100 mL) groups after 14 days of thermal exposure. Glucose concentration surged on the third-day post-temperature challenge, then declined, and was maintained at 35°C until the end of the experiment which was not significant if compared to those at 27°C. Pg0.2 and Mix diets significantly reduced lipid peroxidation and enhanced catalase in gill and liver after 42 days. In the case average water temperature in the Mekong Delta remains below 35°C, the feeding diets for P. hypophthalmus administered Pg0.2 or...

Keywords: Haematology, oxidative stress, Pangasianodon hypophthalmus, Phyllanthus amarus, Psidium guajava

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Abdel-Tawwab, M., & Hamed, H. S. (2020). Antagonistic effects of dietary guava (Psidium guajava) leaves extract on growth, hemato-biochemical, and immunity response of cypermethrin-intoxicated Nile tilapia, Oreochromis niloticus, fingerlings. Aquaculture, 529, 735668.

Adeyemi, O. S., Akanji, M. A., & Ekanem, J. T. (2010). Anti-anaemic properties of the ethanolic extracts of Psidium guajava in Trypanosoma brucei brucei infected rats. Research Journal of pharmacology, 4(3), 74-77.

Barton, B. A. (2002). Stress in fishes: a diversity of responses with particular reference to changes in circulating corticosteroids. Integrative and comparative biology, 42(3), 517-525.

Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry, 72(1-2), 248-254.

Brett, J. R. (1964). The respiratory metabolism and swimming performance of young sockeye salmon. Journal of the Fisheries Board of Canada, 21(5), 1183-1226.

Carvalho, C. S., & Fernandes, M. N. (2006). Effect of temperature on copper toxicity and hematological responses in the neotropical fish Prochilodus scrofa at low and high pH. Aquaculture, 251(1), 109-117.

Cech, J. J., Brauner, C. J., 2011. Respiration: An Introduction. In: Farrell, A.p. (Ed.), Encyclopedia of fish physiology: From genome to environment. Elsevier, pp. 791-795.

Cheng, F. C., Shen, S. C., & Wu, J. S. B. (2009). Effect of guava (Psidium guajava L.) leaf extract on glucose uptake in rat hepatocytes. Journal of food science, 74(5), H132-H138.

Chowdhury, S., & Saikia, S. K. (2020). Oxidative stress in fish: a review. Journal of Scientific Research, 12(1), 145-160.

Crockett, E. L. (2008). The cold but not hard fats in ectotherms: consequences of lipid restructuring on susceptibility of biological membranes to peroxidation, a review. Journal of Comparative Physiology B, 178(7), 795-809.

Dao, L. A. N., Phu, T. M., Douny, C., Quetin-Leclercq, J., Hue, B. T. B., Bach, L. T., ... & Scippo, M. L. (2020). Screening and comparative study of in vitro antioxidant and antimicrobial activities of ethanolic extracts of selected Vietnamese plants. International Journal of Food Properties, 23(1), 481-496.

Donaldson, M. R., Cooke, S. J., Patterson, D. A., & Macdonald, J. S. (2008). Cold shock and fish. Journal of Fish Biology, 73(7), 1491-1530.

Fatima, M., Ahmad, I., Sayeed, I., Athar, M., & Raisuddin, S. (2000). Pollutant-induced over-activation of phagocytes is concomitantly associated with peroxidative damage in fish tissues. Aquatic Toxicology, 49(4), 243-250.

Fawole, F. J., Sahu, N. P., Nazeemashahul, S., & Adeoye, A. A. (2018). Effect of Psidium guajava and Mangifera indica leaves extracts on growth, antioxidant and metabolic enzymes activities of Labeo rohita fingerlings. Journal of the Association of Nigerian Fisheries Scientists, 1, 40-49.

Ferdous, J., Hossain, M. M. M., Jaman, M. H. U., Rupom, A. H., Tonny, N. I., & Jaman, A. (2017). Psidium guajava leaf extracts fed to mono-sex Nile tilapia Oreochromis niloticus enhance immune response against Pseudomonas fluorescens. European Journal of Clinical and Biomedical Sciences, 3(1), 34-42.

Fry, F. E. J. (1971). The effect of environmental factors on the physiology of fish. In Fish physiology (Vol. 6, pp. 1-98). Academic press.

Fuzzen, M. L. M., Bernier, N. J., Kraak, G. V. D., 2011. Stress and Reproduction. In: Norris, D. O., Lopez, K. H. (Eds.), Hormones and Reproduction of Vertebrates. Academic Press, pp. 103-118

Galloway, B. J., & Kieffer, J. D. (2003). The effects of an acute temperature change on the metabolic recovery from exhaustive exercise in juvenile Atlantic salmon (Salmo salar). Physiological and Biochemical Zoology, 76(5), 652-662.

Gobi, N., Ramya, C., Vaseeharan, B., Malaikozhundan, B., Vijayakumar, S., Murugan, K., & Benelli, G. (2016). Oreochromis mossambicus diet supplementation with Psidium guajava leaf extracts enhance growth, immune, antioxidant response and resistance to Aeromonas hydrophila. Fish & Shellfish Immunology, 58, 572-583.

Goth, L. (1991). A simple method for determination of serum catalase activity and revision of reference range. Clinica Chimica Acta, 196(2-3), 143-151.

Gupta, V. K., & Sharma, S. K. (2006). Plants as natural antioxidants. Natural Product Radiance 5(4), 326-334.

Halliwell, B., & Gutteridge, J. M. (2015). Free radicals in biology and medicine. Oxford university press, USA.

Hedayati, A., & Tarkhani, R. (2014). Hematological and gill histopathological changes in iridescent shark, Pangasius hypophthalmus (Sauvage, 1878) exposed to sublethal diazinon and deltamethrin concentrations. Fish Physiology and Biochemistry, 40(3), 715-720.

Hocutt, C. H. (1973). Swimming performance of three warm water fishes exposed to a rapid temperature change. Chesapeake Science, 14(1), 11-16.

Huggett, A. S. G. (1957). Enyzmic determination of blood glucose. Biochem. J., 66, 12.

Huong, D. T. T, Ha, N. T. K., Ngoc, N. M., Em, N. T, Toyoji Kaneko, Phuong, N. T. (2020). Effects of temperature on physiology, growth performance, and digestive enzyme activity of striped catfish (Pangasianodon hypophthalmus) from fry to small fingerling stage. Can Tho University Journal of Science, 56(1-11) (In Vietnamese).

Hurst, T. P. (2007). Causes and consequences of winter mortality in fishes. Journal of Fish Biology, 71(2), 315-345.

Imsland, A. K., Björnsson, B. T., Gunnarsson, S., Foss, A., & Stefansson, S. O. (2007). Temperature and salinity effects on plasma insulin-like growth factor-I concentrations and growth in juvenile turbot (Scophthalmus maximus). Aquaculture, 271(1-4), 546-552.

IPCC (2013). Climate change 2013: The Physical Science Basis. Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change. Intergovernmental Panel on Climate change, Working Group I contribution to the IPCC Fifth Assessment Report (AR5). Cambridge Univ Press, New York, 1535.

Islam, M. A., Uddin, M. H., Uddin, M. J., & Shahjahan, M. (2019). Temperature changes influenced the growth performance and physiological functions of Thai pangas Pangasianodon hypophthalmus. Aquaculture Reports, 13, 100179.

Ju, Z., Dunham, R. A., & Liu, Z. (2002). Differential gene expression in the brain of channel catfish (Ictalurus punctatus) in response to cold acclimation. Molecular Genetics and Genomics, 268(1), 87-95.

Kamath, J. V., Rahul, N., Kumar, C. A., & Lakshmi, S. M. (2008). Psidium guajava L: A review. International Journal of Green Pharmacy (IJGP), 2(1).

Kemp, J. O. G. (2009). Effects of temperature and salinity on resting metabolism in two South African rock pool fish: the resident gobiid Caffrogobius caffer and the transient sparid Diplodus sargus capensis. African Zoology, 44(2), 151-158.

Kim, S. Y., Kim, E. A., Kim, Y. S., Yu, S. K., Choi, C., Lee, J. S., ... & Jeon, Y. J. (2016). Protective effects of polysaccharides from Psidium guajava leaves against oxidative stresses. International Journal of Biological Macromolecules, 91, 804-811.

Laily, N., Kusumaningtyas, R. W., Sukarti, I., & Rini, M. R. D. K. (2015). The potency of guava Psidium guajava (L.) leaves as a functional immunostimulatory ingredient. Procedia Chemistry, 14, 301-307.

Lakshmi, B. V. S., & Sudhakar, M. (2009). Screening of Psidium guajava leaf extracts for antistress activity in different experimental animal models. Pharmacognosy research, 1(6).

Larsen, H. N., & Snieszko, S. F. (1961). Modification of the microhematocrit technique with trout blood. Transactions of the American Fisheries Society, 90(2), 139-142.

Law of animal health, 2015. Article 21 - Vietnam national assembly Passes animal health law – Date: 10/7/2015, Report number: VM5068.

Lesser, M. P. (2006). Oxidative stress in marine environments: biochemistry and physiological ecology. Annual Review of Physiology, 68, 253-278.

Lushchak, V. I. (2011). Environmentally induced oxidative stress in aquatic animals. Aquatic Toxicology, 101(1), 13-30.

MARD_Ministry of Agriculture and Rural Development (2022). Tra fish exports towards the target of over 1.6 billion USD in 2022. Accessed on 8/5/2022.

MONRE (Ministry of Natural Resources and Environment) (2009). Scenarios for climate change, sea level rise for Vietnam. 34 pages.

Maduinyi, I. (1983). Biochemical and pharmiocol studies of the active principles of the seeds of Garcinia kola (Doctoral dissertation, University of Nigeria Nsukka, Nigeria).

Nantitanon, W., Yotsawimonwat, S., & Okonogi, S. (2010). Factors influencing antioxidant activities and total phenolic content of guava leaf extract. LWT-Food Science and Technology, 43(7), 1095-1103.

Natt, M. P., & Herrick, C. A. (1952). A new blood diluent for counting the erythrocytes and leucocytes of the chicken. Poultry Science, 31(4), 735-738.

Nhu, T. Q., Bich Hang, B. T., Cornet, V., Oger, M., Bach, L. T., Anh Dao, N. L., ... & Kestemont, P. (2020). Single or combined dietary supply of Psidium guajava and Phyllanthus amarus extracts differentially modulate immune responses and liver proteome in striped catfish (Pangasianodon hyphophthalmus). Frontiers in Immunology, 11, 797.

Nhu, T. Q., Hang, B. T. B., Vinikas, A., Hue, B. T. B., Quetin-Leclercq, J., Scippo, M. L., ... & Kestemont, P. (2019). Screening of immuno-modulatory potential of different herbal plant extracts using striped catfish (Pangasianodon hypophthalmus) leukocyte-based in vitro tests. Fish & Shellfish Immunology, 93, 296-307.

Oh, W. K., Lee, C. H., Lee, M. S., Bae, E. Y., Sohn, C. B., Oh, H., ... & Ahn, J. S. (2005). Antidiabetic effects of extracts from Psidium guajava. Journal of Ethnopharmacology, 96(3), 411-415.

Omitoyin, B. O., Ajani, E. K., Orisasona, O., Bassey, H. E., Kareem, K. O., & Osho, F. E. (2019). Effect of guava Psidium guajava (L.) aqueous extract diet on growth performance, intestinal morphology, immune response and survival of Oreochromis niloticus challenged with Aeromonas hydrophila. Aquaculture Research, 50(7), 1851-1861.

Pachanawan, A., Phumkhachorn, P., & Rattanachaikunsopon, P. (2008). Potential of Psidium guajava supplemented fish diets in controlling Aeromonas hydrophila infection in tilapia (Oreochromis niloticus). Journal of Bioscience and Bioengineering, 106(5), 419-424.

Phuc, N. T. H. (2015). Effects of temperature and salinity on growth performance in cultured tra catfish (Pangasianodon hypophthalmus) in Vietnam (Doctoral dissertation, Queensland University of Technology), 159 pages.

Portner, H. O., Berdal, B., Blust, R., Brix, O., Colosimo, A., De Wachter, B., ... & Zakhartsev, M. (2001). Climate induced temperature effects on growth performance, fecundity and recruitment in marine fish: developing a hypothesis for cause and effect relationships in Atlantic cod (Gadus morhua) and common eelpout (Zoarces viviparus). Continental Shelf Research, 21(18-19), 1975-1997.

Portner, H. O. (2011). Cellular Energy Utilization: Environmental Influences on Metabolism, in: Farrell, A.P. (Ed.), Encyclopedia of Fish Physiology: From Genome To Environment. Academic Press, pp. 1645-1651.

Roberts, T. R., & Vidthayanon, C. (1991). Systematic revision of the Asian catfish family Pangasiidae, with biological observations and descriptions of three new species. Proceedings of the Academy of Natural Sciences of Philadelphia, 97-143.

Roche, H., & Bogé, G. (1996). Fish blood parameters as a potential tool for identification of stress caused by environmental factors and chemical intoxication. Marine Environmental Research, 41(1), 27-43.

Santos, M. A., & Pacheco, M. (1996). Anguilla anguillaL. Stress Biomarkers Recovery in Clean Water and Secondary-Treated Pulp Mill Effluent. Ecotoxicology and Environmental Safety, 35(1), 96-100.

Semren, T. Ž., Žunec, S., & Pizent, A. (2018). Oxidative stress in triazine pesticide toxicity: a review of the main biomarker findings. Archives of Industrial Hygiene and Toxicology, 69(2), 109-125.

Shahjahan, M., Uddin, M., Bain, V., & Haque, M. (2018). Increased water temperature altered hemato-biochemical parameters and structure of peripheral erythrocytes in striped catfish Pangasianodon hypophthalmus. Fish Physiology and Biochemistry, 44(5), 1309-1318.

Shakeera, B. M., Sujatha, K., Sridharan, G., & Manikandan, R. (2013). Antihyperglycemic and antihyperlipidemic potentials of Psidium guajava in alloxan-induced diabetic rats. Asian Journal of Pharmaceutical and Clinical Research, 6(2), 88-89.

Shen, S. C., Cheng, F. C., & Wu, N. J. (2008). Effect of guava (Psidium guajava Linn.) leaf soluble solids on glucose metabolism in type 2 diabetic rats. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 22(11), 1458-1464.

Snellgrove, D. L., & Alexander, L. G. (2011). Haematology and plasma chemistry of the red top ice blue mbuna cichlid (Metriaclima greshakei). British Journal of Nutrition, 106(S1), S154-S157.

Soltanian, S., Adloo, M. N., Hafeziyeh, M., & Ghadimi, N. (2014). Effect of β-Glucan on cold-stress resistance of striped catfish, Pangasianodon hypophthalmus (Sauvage, 1878). Veterinarni Medicina, 59(9), 440-446.

Thinh, P. V., Phuong, N. T., Huong, D. T. T., & Phuc, N. T. H. (2014). The effects of temperature on physiological parameters and growth rate of catfish (Pangasianodon hypophthalmus). Can Tho University Journal of Science, 1, 292-301.

Tobin, D., & Wright, P. J. (2011). Temperature effects on female maturation in a temperate marine fish. Journal of Experimental Marine Biology and Ecology, 403(1-2), 9-13.

Uysal, K., Çolak, Y., & Kavasoğlu, M. (2019). Effects of Water Temperature on Catalase Activity and Malondialdehyde Level in Muscle, Mouth, and Intestine Tissues of Doctor Fish (Garra rufa). Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 23(1), 191-194.

Valko, M., Leibfritz, D., Moncol, J., Cronin, M. T., Mazur, M., & Telser, J. (2007). Free radicals and antioxidants in normal physiological functions and human disease. The international Journal of Biochemistry & Cell Biology, 39(1), 44-84.

Van Weerd, J. H., & Komen, J. (1998). The effects of chronic stress on growth in fish: a critical appraisal. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 120(1), 107-112.

Vinagre, C., Madeira, D., Narciso, L., Cabral, H. N., & Diniz, M. (2012). Effect of temperature on oxidative stress in fish: lipid peroxidation and catalase activity in the muscle of juvenile seabass, Dicentrarchus labrax. Ecological Indicators, 23, 274-279.

Ward, D. L., & Bonar, S. A. (2003). Effects of cold water on susceptibility of age-0 flannelmouth sucker to predation by rainbow trout. The Southwestern Naturalist, 43-46.

Wendelaar-Bonga, S. E. (2011). Hormonal response to stress. In: Farrel, A.P. (Ed.), Encyclopedia of Fish: Fish Physiology From Genome to Environment. Academic Press, pp. 1515-1523.

Zijlstra, W. G., Buursma, A., & Zwart, A. (1983). Molar absorptivities of human hemoglobin in the visible spectral range. Journal of Applied Physiology, 54(5), 1287-1291.

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