Thi Bach Le , Le Tien Dung , Nguyen Trong Tuan , Do Xuan Canh , Lam Chan Du , Bui Ngoc Anh , Nguyen Hoang Thien Long , Tran Nguyen Gia Huy and Bui Thi Buu Hue *

* Correspondence: Bui Thi Buu Hue (email:

Main Article Content


Guava, Psidium guajava L., is well-known tropic tree which is abundantly grown for fruit. It belongs to phylum Magnoliophyta, class Magnoliopsida and Myrtaceae family and has been found a lot in the Mekong Delta of Viet Nam. Antioxidant activity of different extracts from Psidium guajava L. leaves revealed that ethyl acetate and n-butanol extracts have the good activities. Processing of these fractions using repeated column and thin layer chromatographic techniques resulted in the isolation of four compounds, which have identified their chemical structures by spectroscopic techniques including avicularin (1), kaempferol (2), gallic acid (3), and protocatechuic acid (4). All the isolated compounds showed antioxidant activity using DPPH and ABTS scavenging assays. These results suggest that the leaves of guava  would be useful natural sources of antioxidants.

Keywords: Psidium guajava L., flavonoid, phenolic compound, antioxidant activity

Article Details


Abdullah, N. H., Salim, F., & Ahmad, R. (2016). Isolation of flavonols from the stems of Malaysian Uncaria cordata var. Ferruginea (Blume) RIDSD. Malaysian Journal of Analytical Sciences, 20(4), 844-848.

Barbalho, S. M., Farinazzi-Machado, F. M. V., de Alvares Goulart, R., Brunnati, A. S., Otoboni, A. M., & Ottoboni, B. (2012). Psidium guajava (Guava): A plant of multipurpose medicinal applications. Medicinal and Aromatic Plants Research Journals, 1(4), 1-6.

Biswas, B., Rogers, K., McLaughlin, F., Daniels, D., & Yadav, A. (2013). Antimicrobial activities of leaf extracts of guava (Psidium guajava L.) on two Gram-negative and Gram-positive bacteria. International Journal of Microbiology, 2013, 1-7.

Dawidar A., Abdel-Mogib M., El-Naggar M., & Mostafa M. (2014). Isolation and characterization of Polygonum equisetiforme flavonoids and their acaricidal activity against Tetranychus urticae Koch, Research Journal of Pharmaceutical, Biological and Chemical Sciences, 5(4), 140-148.

Heim, K. E., Tagliaferro, A. R., & Bobilya, D. J. (2002). Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. The Journal of Nutritional Biochemistry, 13(10), 572-584.

Khanh, P. N., Đuc, H. V., Huong, T. T., Ha, V. T., Van, D. T., Kim, Y. H., & Cuong, N. M. (2016). Phenolic compounds from Callistemon citrinus leaves and stems. Vietnam Journal of Science and Technology, 54(2), 190-197.

Mahfuzul Hoque, M. D., Bari, M. L., Inatsu, Y., Juneja, V. K., & Kawamoto, S. (2007). Antibacterial activity of guava (Psidium guajava L.) and neem (Azadirachta indica A. Juss.) extracts against foodborne pathogens and spoilage bacteria. Foodborne Pathogens and Disease, 4(4), 481-488.

Ngbolua, J. P. K. T. N. (2018). A review on the phytochemistry and pharmacology of Psidium guajava L. (Myrtaceae) and future direction. Phytomedicine, 5(2), 7-13.

Naseer, S., Hussain, S., Naeem, N., Pervaiz, M., & Rahman, M. (2018). The phytochemistry and medicinal value of Psidium guajava (guava). Clinical Phytoscience, 4(1), 1-8.

Nenadis, N., Wang, L. F., Tsimidou, M., & Zhang, H. Y. (2004). Estimation of scavenging activity of phenolic compounds using the ABTS•+ assay. Journal of Agricultural and Food Chemistry, 52(15), 4669-4674.

Rawi, S. M., Mourad, I. M., & Sayed, D. A. (2011). Biochemical changes in experimental diabetes before and after treatment with mangifera indica and Psidium guava extracts. International Journal of Pharmacy and Biological Sciences, 2(2), 29-41.

Sharma, O. P., & Bhat, T. K. (2009). DPPH antioxidant assay revisited. Food Chemistry, 113(4), 1202-1205.

Silva da L. A. L., Faqueti L. G., Reginatto F. H., dos Santos A. D. C., Barison A., & Biavatti M. W. (2015). Phytochemical analysis of Vernonanthura tweedieana and a validated UPLC-PDA method for the quantification of eriodictyol, Revista Brasileira de Farmacognosia, 25(4), 375-381.

Seyoum, A., Asres, K., & El-Fiky, F. K. (2006). Structure–radical scavenging activity relationships of flavonoids. Phytochemistry, 67(18), 2058-2070.

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