A simple spectrophotometric method for quantifying total lipids in plants and animals

Tran Thanh Men * , Dai Thi Xuan Trang , Tran Duy Binh , Nguyen Quoc Chau Thanh and Nguyen Dinh Hai Yen

* Corresponding author (ttmen@ctu.edu.vn)

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Received: 03 Jan 2019
Revised: 20 May 2019
Accepted: 30 Aug 2019
Published: 31 Jul 2019
DOI: 10.22144/ctu.jen.2019.030
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Tran, T. M., Dai, T. X. T., Tran, D. B., Nguyen, Q. C. T., & Nguyen, D. H. Y. (2019). A simple spectrophotometric method for quantifying total lipids in plants and animals. CTU Journal of Innovation and Sustainable Development , 11(2), 106-110. https://doi.org/10.22144/ctu.jen.2019.030
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Vol. 11 No. 2 (2019)

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Abstract

Lipids play important roles for the cell in storing energy, structuring cell membrane and signaling pathway. In numerous distinctive studies, the analysis of lipids is to examine for distinct purposes. In the current research, a reliable, rapid, and economical assay has been developed to quantify the total of lipid in samples. The development of colorimetric sulfo-phospho-vanillin is for high throughput analysis of total lipids. In this method, a reaction mixture is performed in a 96-well microplate. The advantages provided from this new assay over other lipid measurement methods, including only small amount of sample requirement for fitting in the standard range, less time requirement and labor in analysing a large number of sample, and the more consistent of color development between lipid content and reagent concentration.
Keywords: Measurement, sulfo-phospho-vanillin, total lipid

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