Bioactive compounds, pigment content and antioxidant activity of pouzolzia zeylanica plant collected at different growth stages

Nguyen Duy Tan * , Vo Thi Xuan Tuyen and Nguyen Minh Thuy

* Corresponding author (ndtan@agu.edu.vn)

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Received: 23 May 2018
Revised: 01 Aug 2018
Accepted: 03 Aug 2018
Published: 24 Aug 2018
DOI: 10.22144/ctu.jsi.2018.095
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Nguyen, D. T., Vo, T. X. T., & Nguyen, M. T. (2018). Bioactive compounds, pigment content and antioxidant activity of pouzolzia zeylanica plant collected at different growth stages. CTU Journal of Innovation and Sustainable Development , 54(Special issue on Agriculture), 54-61. https://doi.org/10.22144/ctu.jsi.2018.095
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No. Special issue on Agriculture (2018)

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Abstract

The aim of this study was to investigative the effect of different growth stages of Pouzolzia zeylanica plant (young and mature plant) on bioactive compounds content (anthocyanin, flavonoid, polyphenol and tannin); the content of pigments (chlorophyll a, chlorophyll b, total chlorophyll and carotenoids); antioxidant activity through some measurable values such as antioxidant ability index (AAI), ferrous reducing ability power (FRAP) and scavenging capacity 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical; as well as the color parameters of stem and leaves (L*, a*, b* and DE). The results showed that the content of anthocyanin, flavonoid, polyphenol, chlorophyll a, chlorophyll b, total chlorophyll and carotenoids of young plant was higher than that of mature ones, and had statistically significant difference (P<0.05), but the content of tannin was not different between the two groups (P>0.05). There was no difference in antioxidant capacity between young and mature plants when performed with scavenging free radical (DPPH) or total reducing power (AAI), but there was significant difference when performed with ferrous reducing ability power (FRAP) method. In addition, there were also statistically significant differences in average values of a* and b* between young and mature, stem and leaves, and these two parameters were related to the content of anthocyanin, chlorophyll, carotenoid in Pouzolzia zeylanica plant.
Keywords: Antioxidant activity, bioactive compounds, growth stage, pigments, Pouzolzia zeylanica

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References

Adedapo, A.A., Jimoh, F.O., Afolayan, A.J. and Masika, P.J., 2009. Antioxudant Properties of the Methanol Extracts of the Leaves and Stems of Celtis africana. Records of Natural Products, 3 (1): 23-31.

Adhikari, B.S. and Babu, M.M., 2008. Floral diversity of Banganga Wetland, Uttarakhand, India. Check list 4 (3): 279-290.

Ahmed, J.K., Salih, H.A.M. and Hadi, A.G., 2013. Anthocyanin in red beet juice act as scavenger for heavy metals ions such as lead and cadmium. International Jouranl of Science and Technology, 2 (3): 269-273.

Aluko, B.T., Alli, S.Y.R. and Omoyeni, O.A., 2014. Phytochemical analysis and antioxidant activities of ethanolic leaf extract of Brillantaisia patula. World Journal of Pharmaceutical Research, 3 (3): 4914-4924.

Bhattacharjya, D.K. and Borah, P.C., 2008. Medicinal weeds of crop fields and role of women in rural health and hygiene in Nalbari district, Assam. Indian Journal of Traditional Knowledge, 7 (3): 501-504.

Eswari, M.L., Bharathi, R.V. and Jayshree, N., 2013. Preliminary phytochemical screening and heavy metal analysis of leaf extracts of Ziziphusoenoplia (L) Mill. Gard. International Journal of Pharmaceutical Sciences and Drug Research, 5 (1): 38-40.

Ghani, 2003. Medicinal plants of Bangladesh: Chemical constituents and uses, 2nd Ed. pp. 104, The Asiatic Society of Bangladesh, Dhaka.

Hossain, M.A., Raqmi, K.A.S., Mijizy, Z.H., Weli, A.M. and Riyami, Q., 2013. Study of total phenol, flavonoids contents and phytochemical sreening of various leaves crude extracts of locally grown Thymus vularis. Asian Pacific Journal of Tropical Biomedicine 3 (9): 705-710.

Jakovljevic, Z.D., Stankovic, S.M. and Topuzovic, D.M., 2013. Sseasonal variability of Chelidonium majus L. secondary metabolites content and antioxidant activity. EXCLI Journal, 12: 260-268

Laitonjam, W.S., Yumnam, R., Asem, S.D. and Wangkheirakpam, S.D., 2013. Evaluative and comparative study of biochemical, trace elements and antioxidant activity of Phlogacanthus pubinervius T. Anderson and Phlocanthus jenkincii C.B. Clarke leaves. Indian Journal of Natural Products and Resources, 4 (1): 67-72.

Le Thanh Thuy, 2007. Preliminary test of chemical components for Pouzolzia zeylanica L. Benn. Chemical Master Thesis, University of Natural Science, HCM city (in Vietnamese).

Lee, J., Durst, R.W. and Wrolstad, R.E., 2005. Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: Collaborative study.Journal of AOAC International, 88 (5): 1269-1278.

Li, P., Huo, L., Su, W., et al., 2011. Free radical-scavenging capacity, antioxidant activity and phenolic content of Pouzolzia zeylanica. Journal of the Serbian Chemical Society, 76 (5): 709-717.

Li, T.S.C., 2006. Taiwanese Native Medicinal Plants, Phytopharmacology and Therapeutic Values. CRC Taylor & Francis Group, LLC.

Loranty, A., Rembiałkowska, E., Rosa, E.A. and Bennett, R.N., 2010. Identification, quantification and availability of carotenoidsand chlorophylls in fruit, herb and medicinal teas. Journal of Food Composition Analysis, 23 (5): 432-441.

Mandal, S., Patra, A., Samanta, A., et al., 2013. Analysis of phytochemical profile of Terminalia arjuna bark extract with antioxidative and antimicrobial properties. Asian Pacific Journal of Tropical Biomedicine 3 (12): 960-966.

Mondal, P., Bhuyan, N., Das, S., Kumar, M., Barah, S. and Mahato, K., 2013. Herbal medicines useful for the treatment of diabetes in North-East India: A review. International Journal of Pharmacy and Biological Sciences, 3 (1): 575-589.

Nguyen Thi Minh Tu, 2009. Study on isolation active compounds from Lingzhi (Ganoderma lucidium). Journal of Science and Technology, Vietnam Academy of Science and Technology, 47 (1): 45-53 (in Vietnamese).

Nhuan, D.T. and Hwang, E.S., 2014. Bioactive Compound Contents and Antioxidant Activity in Aronia (Aronia melanocarpa) Leaves Collected at Different Growth Stages. Prev. Nutr. Food Sci., 19 (3): 204-212

Paul, S. and Saha, D., 2012. In vitro screening of cytotoxic activities of ethanolic extract of Pouzolzia zeylanica (L.) Benn. International Journal of Pharmaceutical Innovations (IJPI), 2 (1): 52-55.

Purkayastha, J., Dutta, M. and Nath, S.C., 2007. Ethnomedicinal plant from Dibru-Saikhowa biosphere reserve, Assam. Indian Journal of Traditional Knowledge, 6 (3): 477-480.

Ratnam, K.V. and Raju, R.R., 2008. Traditional medicine used by the adivasis of Eantern Ghats, andhra pradesh – for bone fractures. Ethnobotanical leaflets, 12: 19-22. 2008.

Rhim, J., Wu, Y., Weller, C. and Schnepf, M., 1999. Physical characteristics of a composite film of soy protein isolate and propyleneglycol alginate. Journal of Food Science, 64 (1): 149–152.

Saha, A.K., Rahman, M.R., Shahriar, M., Saha, S.K., Azad, N.S. and Das, S., 2013. Screening of six Ayurvedic Medicinal Plant Extracts for Antioxidant and Cytotoxic Activity. Journal of Pharmacognosy and Phytochemistry, 2 (2): 181-188.

Saha, D. and Paul, S., 2012a. Studies on Pouzolzia zeylanica (L.) Benn. (Family: Urticaceae). Lap Lambert Academic Publishing, Germany.

Saha, D. and Paul, S., 2012b. Antifungal activity of ethanol extract of Pouzolzia zeylanica (L.) Benn. International Journal of Pharmacy Teaching and Pratices, 3 (2): 272-274.

Saha, D., Paul, S. and Chowdhury, S., 2012. Antibacterial activity of ethanol extract of Pouzolzia zeylanica (L.) Benn. International Journal of Pharmaceutical Innovations (IJPI), 2 (1): 1-5.

Sandhya, S., Venkata, R.K., Vinod, K.R., Swapna, R. and Asia, B., 2013. Scope of medicinal flora as effective anti ulcer agents. African Journal of Plant Science, 7 (11): 504-512.

Sarma, I.S. and Dinda, B., 2013. A new friedelane triterpene ester from Pouzolzia indica. Indian Journal of Chemistry, 52B: 1527-1530.

Shuib, N.H., Shaari, K., Khatib, et al., 2011. Discrimination of young and mature leaves of Melicope ptelefolia using 1H NMR and multivariate data analysis. Food Chem, 126: 640–645

Singh, A., Lawrence, K., Prandit, S. and Lawrence, R.S., 2014. Response of leaves, stems and roots of Withania somnifera to copper stress. International Journal of Plant, Animal, and Environmental Sciences, 4 (3): 60-67.

Stankovic, M.S., Niciforovic, N., Topuzovic, M and Solujic, S., 2011. Total phenolic content, flavonoid concentrations and antioxidant activity of the whole plant and plant parts extracts from Teucrium montanum L. var. montnum, f. supinum (L.) Reichenb. Biotechnol Biotechnol Eq, 25: 2222-2227.

Stankovic, M.S., Topuzovic, M., Solujic, S. and Mihailovic, V., 2010. Antioxidant activity and concentration of phenols and flavonoids in the whole plant and plant parts of Teucrium chamaedrys L. var. glanduliferum Haussk. J Med Plant Res, 4: 2092-2098.

Tarhan, S., Telci, I., Tuncay, M.T. and Polatci, H., 2010. Product quality and energy consumption when drying peppermint by rotary drum dryer. Industrial Crops and Products, 32 (3): 420-427.

Tran Thi My Tien, Nguyen Thi Thu Huong, Duong Thi Mong Ngoc, Tran Cong Luan and La Van Kinh, 2010. Experimental study on some pharmacological effects of Pouzolzia zeylanica and Tinospora crispa. Medicine Journal of HCM city, special subject Traditional medicine. 14 (2): 116-120 (in Vietnamese).

Vallejo, F., García-Viguera, C. and Tomás-Barberán, F.A., 2003. Changes in Broccoli (Brassica oleracea L. Var. Italica) health-promoting compounds with inflorescence development. J Agric Food Chem, 51: 3776-3782.

Van den Berg, H., Faulks, R., Fernando-Granado, H., et al., 2000. The potential for the improvement of carotenoid levels in foods and the likely systemic effects. J Sci Food Agric 80: 880-912.

Yusuf, M., Wahab, M.A., Chowdhury, J.U. and Begum, J., 2006. Ethno-medico biotanical knowledge from kaukhali proper and betbunia of rangamati district. Bangladesh Journal Plant Taxon, 13 (1): 55-61.

Znidarcic, D., Ban, D. and Sircelj, H., 2011. Carotenoid and chlorophyll composition of commonly consumed leafy vegetables in Mediterranean countries. Food Chem., 129: 1164-1168.

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