Optimization of polyphenol, flavonoid and tannin extraction conditions from Pouzolzia zeylanica L. benn using response surface methodology

Nguyen Duy Tan * , Le Quoc Viet , Vo Tan Thanh and Nguyen Minh Thuy

* Corresponding author (ndtan@agu.edu.vn)

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Received: 27 Jan 2016
Revised: 27 Apr 2016
Accepted: 30 Mar 2017
Published: 31 Mar 2017
DOI: 10.22144/ctu.jen.2017.015
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Nguyen, D. T., Le, Q. V., Vo, T. T., & Nguyen, M. T. (2017). Optimization of polyphenol, flavonoid and tannin extraction conditions from Pouzolzia zeylanica L. benn using response surface methodology. CTU Journal of Innovation and Sustainable Development , (05), 122-131. https://doi.org/10.22144/ctu.jen.2017.015
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No. 05 (2017)

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Abstract

In this study, the extraction of phenolic compounds from Pouzolzia Zeylanica L. Benn was conducted by using pure water as a solvent. The optimal conditions for the extraction of three phenolic compounds such as polyphenols, flavonoids and tannins were determined by using response surface methodology (RSM). A central composite design (CCD) was applied to investigate the effects of three independent variables, namely the ratio of water-to-dried material (20:1 to 30:1, v/w), temperature (70 to 90oC ) and time extraction (20 to 40 minutes). The dependent variables were total polyphenol content (TPC), total flavonoid content (TFC) and tannin content (TC). A second-order polynomial model was used for predicting the response. Optimized conditions for bioactive compounds extraction, water-to-dried material ratio, time and temperature extraction were 27 (v/w), 30 minutes and 81oC, respectively. The experimental values agreed with predicted values within a 95% confidence interval. Total polyphenol, flavonoid and tannin content extracted by these optimized conditions were achieved (521 mgGAE/100g dried material (DM), 363 mgQE/100g DM and 643 mgTAE/100g DM, respectively).
Keywords: Extraction, phenolic compounds, pouzolzia zeylanica L. benn, optimization, response surface methodology

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