Effects of Psidium guajava and Phyllanthus amarus extracts on digestive enzyme activity and growth of Pangasianodon hypophthalmus fingerlings under high-temperature stress
,
,
,
,
,
,
and
*
Article Sidebar
Full Text: 
PDF
Main Article Content
Abstract
The rise in water temperature by global warming is of high concern to aquaculturists. In this study, the effects of extracts-based diets on digestive enzymes, and growth performance in Pangasianodon hypophthalmus fingerlings under elevated temperatures were investigated. Four distinct diets (control, Psidium guajava, (0.2%/kg) – Pg0.2, Phyllanthus amarus (0.5%/kg) – Pa0.5, and a mixture of Pg0.2 and Pa0.5 - Mix.) were administered to fish fingerlings for 42 days, followed by 4 days of temperature elevation. Fish were then continuously subjected for 42 days to temperatures of 27, 31, and 35°C to evaluate enzymatic activities and growth performance of fish. The results showed that although there is no interaction between two experimental factors on digestive enzyme activity and growth performance of fish, Pg0.2 followed by Mix groups accelerated digestive enzymes (trypsin, chymotrypsin, amylases and pepsin). Besides, enzymatic activities increased from 31°C to 35°C. The highest growth was observed from fish at 35°C followed by those at 31°C (p<0.05) which was significantly higher than the control (27°C); however, there was no significant difference in survival rate. In conclusion, these findings suggested 2 appropriate diets (Pg0.2 and Mix) for optimizing growth of this species and consequently contributing to the sustainable aquaculture under the global warming scenario.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
References
Abdelhamid, A. M., & Soliman, A. A. A. (2012). Possibility of using dried leaves of guava and camphor trees in tilapia diets. Journal of the Arabian Aquaculture Society, 7(1), 91-108.
Abdel-Tawwab, M., Hagras, A. E., Elbaghdady, H. A. M., & Monier, M. N. (2015). Effects of dissolved oxygen and fish size on Nile tilapia, Oreochromis niloticus (L.): growth performance, whole-body composition, and innate immunity. Aquaculture International, 23(5), 1261-1274.
Abou‐Zeid, S. M. (2002). The effect of some medical plant on reproductive and productive performance of Nile tilapia fish (doctoral dissertation). Cairo University.
Allen, M., Dube, O. P., Solecki, W., Aragón-Durand, F., Cramer, W., Humphreys, S., & Kainuma, M. (2018). Special Report: Global Warming of 1.5 C. Intergovernmental Panel on Climate Change (IPCC).
Bernfeld, P. (1951). Enzymes of starch degradation and synthesis. Advances in enzymology and related areas of molecular biology, 12, 379-428.
Bilen, S., Altief, T. A. S., Özdemir, K. Y., Salem, M. O. A., Terzi, E., & Güney, K. (2020). Effect of lemon balm (Melissa officinalis) extract on growth performance, digestive and antioxidant enzyme activities, and immune responses in rainbow trout (Oncorhynchus mykiss). Fish physiology and biochemistry, 46(1), 471-481.
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry, 72(1-2), 248-254.
Dao, N. L. A., Phu, T. M., Douny, C., Quetin-Leclercq, J., Hue, B. T. B., Bach, L. T., Nhu, T. Q., Hang, B. T. B., Huong, D. T. T., Phuong, N. T., Kestemon, P., & Scippo, M. L. (2020). Screening and comparative study of in vitro antioxidant and antimicrobial activities of ethanolic extracts of selected Vietnamese plants. International Journal of Food Properties, 23(1), 481-496.
Direkbusarakom, S., Ezura, Y., Yoshimizu, M., & Herunsalee, A. (1998). Efficacy of Thai traditional herb extracts against fish and shrimp pathogenic bacteria. Fish pathology, 33(4), 437-441.
Donaldson, M. R., Cooke, S. J., Patterson, D. A., & Macdonald, J. S. (2008). Cold shock and fish. Journal of Fish Biology, 73(7), 1491-1530.
Fairbairn, D. J., Liu, W., Schachtman, D. P., Gomez-Gallego, S., Day, S. R., & Teasdale, R. D. (2000). Characterisation of two distinct HKT1-like potassium transporters from Eucalyptus camaldulensis. Plant molecular biology, 43(4), 515-525.
Ferdous, J., Hossain, M. M. M., Jaman, M. H. U., Rupom, A. H., Tonny, N. I., & Jaman, A. (2017). Psidium guajava leaf extracts fed to mono-sex Nile Tilapia Oreochromis niloticus enhance immune response against Pseudomonas fluorescens. European Journal of Clinical and Biomedical Sciences, 3(1), 3442.
Fry, F. E. J. (1971). The effect of environmental factors on the physiology of fish. Fish physiology, 1-98.
Furné, M., García-Gallego, M., Hidalgo, M. C., Morales, A. E., Domezain, A., Domezain, J., & Sanz, A. (2008). Effect of starvation and refeeding on digestive enzyme activities in sturgeon (Acipenser naccarii) and trout (Oncorhynchus mykiss). Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 149(4), 420-425.
Gabriel, N. N., Qiang, J., Ma, X. Y., Xu, P., & Nakwaya, D. N. (2017). Effects of dietary Aloe vera crude extracts on digestive enzyme activities and muscle proximate composition of GIFT tilapia juveniles. South African Journal of Animal Science, 47(6), 904-913.
Galloway, B. J., & Kieffer, J. D. (2003). The effects of an acute temperature change on the metabolic recovery from exhaustive exercise in juvenile Atlantic salmon (Salmo salar). Physiological and Biochemical Zoology, 76(5), 652-662.
Gildberg, A. R., & Raa, J. (1983). Purification and characterization of pepsins from the arctic fish capelin (Mallotus villosus). Comparative Biochemistry and physiology. A, Comparative Physiology, 75(3), 337-342.
Giri, S. S., Sen, S. S., Chi, C., Kim, H. J., Yun, S., Park, S. C., & Sukumaran, V. (2015). Effect of guava leaves on the growth performance and cytokine gene expression of Labeo rohita and its susceptibility to Aeromonas hydrophila infection. Fish & shellfish immunology, 46(2), 217-224.
Gobi, N., Ramya, C., Vaseeharan, B., Malaikozhundan, B., Vijayakumar, S., Murugan, K., & Benelli, G. (2016). Oreochromis mossambicus diet supplementation with Psidium guajava leaf extracts enhance growth, immune, antioxidant response and resistance to Aeromonas hydrophila. Fish & shellfish immunology, 58, 572-583.
Hocutt, C. H. (1973). Swimming performance of three warmwater fishes exposed to a rapid temperature change. Chesapeake science, 14(1), 11-16.
Huong, D.T.T, Ha, N.T. K., Ngoc, N.M., Em, N.T, Toyoji, K., & Phuong, N.T. (2020). Effects of temperature on physiology, growth and digestive enzyme activity of striped catfish (Pangasianodon hypophthalmus) at fry stage. Science Journal of Can Tho University, 1-11.
Hurst, T. P. (2007). Causes and consequences of winter mortality in fishes. Journal of Fish Biology, 71(2), 315-345.
IPCC. (2021). Climate Change 2021: The Physical Science Basis. Working Group I contribution to the Sixth Assessment Report of the Intergovernmental Panel on Climate Change, Geneva, Switzerland.
Kemp, J. O. G. (2009). Effects of temperature and salinity on resting metabolism in two South African rock pool fish: the resident gobiid Caffrogobius caffer and the transient sparid Diplodus sargus capensis. African Zoology, 44(2), 151-158.
Killen, S. S. (2011). Energetics of foraging decisions and prey handling. In: Farrell, A.P., Stevens, E.D., Cech, J.J., & Richards, J.G. (Eds.) Encyclopedia of Fish Physiology: From Genome to Environment. Academic Press: San Diego.
Kita, J., Tsuchida, S., & Setoguma, T. (1996). Temperature preference and tolerance, and oxygen consumption of the marbled rockfish, Sebastiscus marmoratus. Marine Biology, 125(3), 467-471.
Lutterodt, G. D. (1989). Inhibition of gastrointestinal release of acetylchoune byquercetin as a possible mode of action of Psidium guajava leaf extracts in the treatment of acute diarrhoeal disease. Journal of ethnopharmacology, 25(3), 235-247.
MARD. (2022). Tra fish exports towards the target of over 1.6 billion USD in 2022. https://www.mard.gov.vn/en/Pages/tra-fish-exports-towards-the-target-of-over-1-6-billion-usd-in-2022.aspx
Munilla-Morán, R., & Saborido-Rey, F. (1996). Digestive enzymes in marine species. II. Amylase activities in gut from seabream (Sparus aurata), turbot (Scophthalmus maximus) and redfish (Sebastes mentella). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 113(4), 827-834.
Nhu, T. Q., Dam, N. P., Hang, B. T. B., Hue, B. T. B., Scippo, M. L., Phuong, N. T., Quetin-Leclercq, J., & Kestemont, P. (2020a). Immunomodulatory potential of extracts, fractions and pure compounds from Phyllanthus amarus and Psidium guajava on striped catfish (Pangasianodon hypophthalmus) head kidney leukocytes. Fish & shellfish immunology, 104, 289-303.
Nhu, T. Q., Hang, B. T. B., Vinikas, A., Hue, B. T. B., Huong, D. T. T., Quetin-Leclercq, J., Scippo, M. L., Phuong, N. T., & Kestemont, P. (2019). Screening of immuno-modulatory potential of different herbal plant extracts using striped catfish (Pangasianodon hypophthalmus) leukocyte-based in vitro tests. Fish & shellfish immunology, 93, 296-307.
Nhu, T. Q., Hang, B. T.B., Cornet, V., Oger, M., Bach, L. T., Dao, N. L.A., Huong, D. T. T., Quetin-Leclercq, J., Scippo, M. L., Phuong, N. T.,... & Kestemont, P. (2020b). Single or combined dietary supply of Psidium guajava and Phyllanthus amarus extracts differentially modulate immune responses and liver proteome in striped catfish (Pangasianodon hyphophthalmus). Frontiers in Immunology, 11, 797.
Omitoyin, B. O., Ajani, E. K., Orisasona, O., Bassey, H. E., Kareem, K. O., & Osho, F. E. (2019). Effect of guava Psidium guajava (L.) aqueous extract diet on growth performance, intestinal morphology, immune response and survival of Oreochromis niloticus challenged with Aeromonas hydrophila. Aquaculture Research, 50(7), 1851-1861.
Pachanawan, A., Phumkhachorn, P., & Rattanachaikunsopon, P. (2008). Potential of Psidium guajava supplemented fish diets in controlling Aeromonas hydrophila infection in tilapia (Oreochromis niloticus). Journal of bioscience and bioengineering, 106(5), 419-424.
Phuc, N. T. H. (2015). Effects of temperature and salinity on growth performance in cultured Tra catfish (Pangasianodon hypophthalmus) in Vietnam (Doctoral dissertation). Queensland University of Technology.
Reece, J. B., Urry, L. A., Cain, M. L., Wasserman, S. A., Minorsky, P. V., & Jackson, R. B. (2014). Campbell biology (9th ed.). New York : Benjamin Cummings Pearson Education.
Shabana, M. S., Karthika, M., & Ramasubramanian, V. (2019). Effect of dietary Citrus sinensis peel extract on growth performance, digestive enzyme activity, muscle biochemical composition, and metabolic enzyme status of the freshwater fish, Catla catla. The Journal of Basic and Applied Zoology, 80(1), 1-9.
Thinh, P. V., Phuong, N. T., Huong, D. T. T. and Phuc, N. T. H. (2014). Effects of temperature on physiology and growth of Pangasianodon hypophthalmus. Journal of Science Can Tho University, 1, 292-301.
Tobin, D., & Wright, P. J. (2011). Temperature effects on female maturation in a temperate marine fish. Journal of Experimental Marine Biology and Ecology, 403(1-2), 9-13.
Tseng, H. C., Grendell, J. H., & Rothman, S. S. (1982). Food, duodenal extracts, and enzyme secretion by the pancreas. American journal of physiologygastrointestinal and liver physiology, 243(4), G304-G312.
Volkoff, H., & Rønnestad, I. (2020). Effects of temperature on feeding and digestive processes in fish. Temperature, 7(4), 307-320.
Wang, C. Y., Li, Z. B., Sun, Y. Z., Chen, Q., Li, W. J., Huang, Y. C., & Lu, J. (2018). Effects of Chinese herbal medicines mixture on growth performance digestive enzyme activity immune response of juvenile Japanese seabass, Lateolabrax japonicus. Aquaculture Nutrition, 24(2), 683-693.
Ward, D. L., & Bonar, S. A. (2003). Effects of cold water on susceptibility of age-0 flannel mouth sucker to predation by rainbow trout. The Southwestern Naturalist, 48(1), 43-46.
Worthington, T. M. (1982). Enzymes, and related biochemicals. Biochemical Products Division, Worthington Diagnostic System. Inc., Freehold, New Jersy, USA.