Duong Thuy Yen * and Nguyen Tien Vinh

* Correspondence: Duong Thuy Yen (email: thuyyen@ctu.edu.vn)

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Abstract

Pangasius krempfi is an important catfish species for capture fisheries in the Mekong River basin.  Overexploitation could lead to decreasing genetic diversity of this species. This study was aimed to quantify genetic diversity and structure of P. krempfi in the lower Mekong River using ISSR markers. Samples were collected from two estuaries of Tien (at Binh Dai, Ben Tre, BT) and Hau Rivers (at Cu Lao Dung, Soc Trang, ST). Twenty individuals per location (or group) were analyzed with six ISSR primers, generated a total of 32 bands with the size ranging from 500 – 2200 bp. The two fish groups had similarly moderate levels of genetic diversity. As the whole population, genetic parameters were (mean ± SE) 56.3 ± 3.1% of polymorphic loci, 1.365 ± 0.048 effective number of alleles, 0.215 ± 0.027 expected heterozygosity, and 0.310 ± 0.037 Shannon index. Genetic distance based on Nei’s method between the two groups was 3.4%, accounting for 12% of total genetic variation. Principal coordinate and molecular genetic variance (AMOVA) analyses indicated that the two fish groups were genetically clustered at a low level, suggesting that they can be originated from different spawning groups of the same population.
Keywords: genetic diversity, ISSR, Pangasius krempfi, population structure

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References

Allendorf, F. W., England, P. R., Luikart, G., Ritchie, P. A., and Ryman, N., 2008. Genetic effects of harvest on wild animal populations. Trends in Ecology and Evolution, 23(6): 327–337.

Baird, I., 1996. Khone falls fishers. Catch and Culture: Mekong Fisheries Network Newsletter, 2(2): 2-3.

Baird, I., 2013. Pangasius krempfi. The IUCN Red List of Threatened Species: e.T181328A7668262.

Bornet, B., and Branchard, M., 2001. Nonanchored inter simple sequence repeat (ISSR) markers: reproducible and specific tools for genome fingerprinting. Plant Molecular Biology Reporter, 19 (3): 209–215.

Braga-Silva, A., and Galetti, P. M., 2016. Evidence of isolation by time in freshwater migratory fish Prochilodus costatus (Characiformes, Prochilodontidae). Hydrobiologia, 765(1): 159–167.

Coulson, M. W., Bradbury, I. R., and Bentzen, P., 2006. Temporal genetic differentiation: Continuous v. discontinuous spawning runs in anadromous rainbow smelt Osmerus mordax (Mitchill). Journal of Fish Biology, 69(SUPPL. C): 209–216.

Tran D.D., Koichi, S., Phương, N. T., et. al., 2013. Fishes of the Mekong Delta, Vietnam. Can Tho University Publishing House.

Duong T. Y., Nguyen Phuong Thao, Tieu Van Ut, and Tran Dac Dinh, 2018. Genetic diversity of kissing gourami (Helostoma temminkii) in the Mekong Delta. Can Tho University Journal of Science, 54(7B): 86–93 (in Vietnamese).

Duong, T. Y., Nguyen, K., Bui, S. N., Nguyen, V. T., Nguyen, B. L., & Tran, D. D., 2016. DNA barcode and morphology of P. krempfi, P. mekongensis, and P. elongatus. Journal of Biotechnology 14(1): 29-37 (in Vietnamese)

Ellegren, H., Galtier, N., and Ellegren, N., 2016. Determinants of genetic diversity. Nature Reviews Genetics, 17(7): 422–433.

Eshak, M. G., Ebeed, N. M., Booles, H. F., and Fahmy, K., 2010. Genetic assessment of salinity tolerance in red tilapia. New York Science Journal, 3(12): 132–141.

Fernandes-Matioli, F.M.C., Matioli, S.R., Almeida-Toledo, L. F., 2000. Species diversity and geographic distribution of Gymnotus (Pisces: Gymnotiformes) by nuclear (GGAC)n microsatellite analysis. Genetics and Molecular Biology, 23(4): 803–807.

Forsythe, P. S., Crossman, J. A., Bello, N. M., Baker, E. A., Scribner, K. T., and Tonn, W., 2012. Individual-based analyses reveal high repeatability in timing and location of reproduction in lake sturgeon (Acipenser fulvescens). Canadian Journal Of Fisheries and Aquatic Science, 69(1): 60–72.

Hogan, Z., Baird, I. G., Radtke, R., and Vander Zanden, M. J., 2007. Long distance migration and marine habitation in the tropical Asian catfish, Pangasius krempfi. Journal of Fish Biology, 71(3): 818–832.

Kumar, S., Stecher, G., and Tamura, K., 2016. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution, 33(7): 1870–1874.

Kumla, S., Doolgindachbaporn, S., Sudmoon, R., et al., 2012. Genetic variation, population structure and identification of yellow catfish, Mystus nemurus (CandV) in Thailand using RAPD, ISSR and SCAR marker. Molecular Biology Reports, 39(5): 5201–5210.

Labastida, E., Cobián, D., Hénaut, Y., et al., 2015. ISSR markers for Pterois species The use of ISSR markers for species determination and a genetic study of the invasive lionfish in Guanahacabibes, Cuba. J. Aquat. Res, 43(5): 1011–1018.

Liu, Y.-G., Chen, S.L., Li, J., 2006. Genetic diversity in three Japanese flounder (Paralichthys olivaceus) populations revealed by ISSR markers. Aquaculture, 255(1): 565–572.

Liu, Z. J., and Cordes, J. F., 2004. DNA marker technologies and their applications in aquaculture genetics. Aquaculture, 238(1-4): 1–37.

Pazza, R., Kavalco, K. F., Prioli, S. M., et al., 2007. Chromosome polymorphism in Astyanax fasciatus (Teleostei, Characidae), Part 3: Analysis of the RAPD and ISSR molecular markers. Biochemical Systematics and Ecology, 35(12):843–851.

Peakall, R., and Smouse, P. E., 2012. GenALEx 6.5: Genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics, 28(19): 2537–2539.

Pham Thi Trang Nhung and Duong Thuy Yen, 2014. Genetic diversity of climbing perch (Anabas testudineus, Bloch 1792) populations based on RAPD and ISSR markers. Can Tho University Journal of Science, 1 (Special issue in Aquaculture): 86–93 (in Vietnamese).

Pinsky, M. L., and Palumbi, S. R., 2014. Meta-analysis reveals lower genetic diversity in overfished populations. Molecular Ecology, 23(1): 29–39.

Poulsen, A. F., and Valbo-Jørgensen, J., 2000. Fish Migrations and spawning habits in the Mekong mainstream: A survey using local knowledge. AMFC Technical Report, (0005), 1–149.

Poulsen, A., and Hortle, K., 2004. Distribution and ecology of some important riverine fish species of the Mekong River Basin. MRC Technical Paper No. 10.

Rainboth, W.J., 1996. Fishes of the Cambodian Mekong, FAO species identification field guide for fishery purposes. Food and Agriculture Organization of the United Nations (FAO), Rome, Italy.

Saad, Y.M., Rashed, M.A., Atta, A.H., et al., 2012. Genetic diversity among some tilapia species based on ISSR markers. Life Science Journal, 9(4): 4841–4846.

Sharma, S. K., Kumaria, S., Tandon, P., et al., 2011. Single primer amplification reaction (SPAR) reveals inter- and intra-specific natural genetic variation in five species of Cymbidium (Orchidaceae). Gene, 483(1–2): 54–62.

Smoker, W. W., Gharrett, A. J., and Stekoll, M. S., 1998. Genetic variation of return date in a population of pink salmon: a consequence of fluctuating environment and dispersive selection? Alaska Fishery Research Bulletin, 5(1): 46–54.

Sokheng, C., Chhea, C.K., Viravong, S., et al., 1999. Fish migrations and spawning habits in the Mekong mainstream: a survey using local knowledge (basin-wide). Assessment of Mekong fisheries: Fish Migrations and Spawning and the Impact of Water Management Project (AMFC). AMFP Report 2/99. Vientiane, Lao P.D.R.

Truong Thu Khoa and Tran Thi Thu Huong, 1993. The freshwater fish species in the Mekong Delta. Faculty of Fisheries. Can Tho University (in Vietnamese).

Yeh, F., Yang, R., and Boyle, T., 1999. Popgene version 1.3.1. Mircosoft Window-based Freeware for Population Genetic Ananlysis. University of Alberta and Centre for International Forestry Research. Edmonton, Alto, 1–29.