Duong Thuy Yen * and Nguyen Tien Vinh

* Correspondence: Duong Thuy Yen (email: thuyyen@ctu.edu.vn)

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Pangasius krempfi is an important catfish species for capture fisheries in the Mekong River basin.  Overexploitation could lead to decreasing genetic diversity of this species. This study was aimed to quantify genetic diversity and structure of P. krempfi in the lower Mekong River using ISSR markers. Samples were collected from two estuaries of Tien (at Binh Dai, Ben Tre, BT) and Hau Rivers (at Cu Lao Dung, Soc Trang, ST). Twenty individuals per location (or group) were analyzed with six ISSR primers, generated a total of 32 bands with the size ranging from 500 – 2200 bp. The two fish groups had similarly moderate levels of genetic diversity. As the whole population, genetic parameters were (mean ± SE) 56.3 ± 3.1% of polymorphic loci, 1.365 ± 0.048 effective number of alleles, 0.215 ± 0.027 expected heterozygosity, and 0.310 ± 0.037 Shannon index. Genetic distance based on Nei’s method between the two groups was 3.4%, accounting for 12% of total genetic variation. Principal coordinate and molecular genetic variance (AMOVA) analyses indicated that the two fish groups were genetically clustered at a low level, suggesting that they can be originated from different spawning groups of the same population.
Keywords: genetic diversity, ISSR, Pangasius krempfi, population structure

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