Nguyen Van Dat * , Huynh Thanh Tuan and Ronny Purwadi

* Corresponding author (nvdat@ctu.edu.vn)

Main Article Content

Abstract

Ion selective electrode (ISE) is a useful tool in the direct determination of ionic species in complex samples. This study relates to the manufacture of Ca2+– selective electrode with single – barrelled microelectrodes and an internal filling solution buffered for pCa (pCa= –lg[Ca2+]) of 6. It is shown that an improved lower detection limit of 10–6 M is obtained in Ca2+ buffer solution and the changes in EMF (Electromotive force) between two solutions of 10 – fold change in Ca2+ concentration are close to 30 mV/decade.
Keywords: Cocktail A, EGTA, EMF, ISE, microelectrodes, SICM

Article Details

References

Ammann, D., Bührer, T., Schefer, U., Müller, M., and Simon, W., 1987. Intracellular neutral carrier–based Ca2+ microelectrode with subnanomolar detection limit. European Journal of Physiology. 409: 223–228.

Ceresa, A., Bakker, E., Hattendorf, B., Gunther, D., and Pretsch, E., 2001. Potentiometrie Polymeric Membrane Electrodes for Measurements of Environmental Samples at Trace Levels: New Requirements for Selectivities and Measuring Protocols, and Comparison with ICPMS. Anal. Chem. 73: 343–351.

Ceresa, A., 2001. Ion–Selective Polymeric Membrane Electrodes for Potentiometric Trace Level Measurements. A dissertation for the degree of Doctor of Natural Sciences. Swiss Federal Institute of Technology (ETH Zürich).

Eriksen, R.S., Mackey, D.J., van Dam, R., and Nowak, B., 2001. Copper speciation and toxicity in Macquarie Harbour, Tasmania: an investigation using a copper ion selective electrode. Mar Chem. 74(2–3): 99–113.

Hernandez, R., Riu, J., and Rius, F.X., 2010. Determination of calcium ion in sap using carbon nanotube ­– based ion – selective electrodes. Analyst. 135(8): 1979–1985.

Lewenstam, A., Maj–Zurawska, M., and Hulanicki, A., 1991. Electroanalysis. Application of ion-selective electrodes in clinical analysis. Electroanalysis. 3(8), 727–734.

Miller, A.J., Cookson, S.J., Smith, S.J. and Wells, D.M., 2001. The use of microelectrodes to investigate compartmentation and the transport of metabolized inorganic ions in plants. Journal of Experimental Botany. 52(356): 541–549.

Ruzica J., Hansen E.H., Tjell, J.C., 1973. Selectrode – the universal ion–selective electrode. Part IV. The calcium (II) selectrode employing a new ion exchanger in a nonporous membrane and solid–state reference system. Anal Chim Aceta 67(1): 155 – 178.

Tsien, R. Y., and Rink, T. J., 1981. Ca2+-selective electrodes: a novel PVC-gelled neutral carrier mixture compared with other currently available sensors. Journal of neuroscience methods, 4(1): 73-86.

Vijayalakshmi, A., and Selvi, J.T., 2017. Calcium Ion Selective Electrode Based on Schiff Base as Ionophore and Determination of Thermodynamic Functions and its Analytical Application. Oriental Journal of Chemistry 33(1): 531–536.

Most read articles by the same author(s)