TRAN DONG AU , Huynh Lien Huong , Ho Quoc Phong * and Nguyen Van Dat

* Correspondence: Ho Quoc Phong (email:

Main Article Content


In this study, transesterfication reaction under subcritical condition of methanol (or called subcritical methanol method) was employed to directly produce biodiesel from chlorella sp. instead of conventional method that based on acid/base catalysts. The effect of reaction parameters on FAME (biodiesel) conversion such as temperature (165 -185 °C), methanol to biomass ratio (10/1-25/1, g/g), water content (5 – 80%), and reaction time (1-12 h) was studied. According to the experimental results, FAME conversion increased with the increase of reaction time and highest FAME conversion 95% at 175 °C, 20/1 (g/g) methanol to biomass ratio and 50% water content in biomass.
Keywords: biodiesel, microalgae, subcritical methanol, transesterification

Article Details


Ho, Q.-P., Tran, D.-A., Tran, S.-N., Thi, N.-H.H., Huynh, L.-H., Nguyen, T.-T., 2014. Lipid production for biodiesel synthesis from Chlorella sp. Can Tho University Journal of Science. 35: 1-8 (In Vietnamese).

Hsieh, C.-H., Wu, W.-T., 2009. Cultivation of microalgae for oil production with a cultivation strategy of urea limitation. Bioresource Technology. 100(17): 3921-3926.

Huynh, L.H., Tran Nguyen, P.L., Ho, Q.P., Ju, Y.-H., 2012. Catalyst-free fatty acid methyl ester production from wet activated sludge under subcritical water and methanol condition. Bioresource Technology. 123(0): 112-116.

Ju, Y.-H., Huynh, L.H., Tsigie, Y.A., Ho, Q.-P., 2013. Synthesis of biodiesel in subcritical water and methanol. Fuel. 105(0): 266-271.

Kusdiana, D., Saka, S., 2004. Effects of water on biodiesel fuel production by supercritical methanol treatment. Bioresource Technology. 91(3): 289-295.

Leung, D.Y.C., Wu, X., Leung, M.K.H., 2010. A review on biodiesel production using catalyzed transesterification. Applied Energy. 87(4): 1083-1095.

Mata, T.M., Martins, A.A., Caetano, N.S., 2010. Microalgae for biodiesel production and other applications: A review. Renewable and Sustainable Energy Reviews. 14(1): 217-232.

Tsigie, Y.A., Huynh, L.H., Ismadji, S., Engida, A.M., Ju, Y.-H., 2012. In situ biodiesel production from wet Chlorella vulgaris under subcritical condition. Chemical Engineering Journal. 213(0): 104-108.

Tsigie, Y.A., Huynh, L.H., Nguyen, P.L.T., Ju, Y.-H., 2013. Catalyst-free biodiesel preparation from wet Yarrowia lipolytica Po1g biomass under subcritical condition. Fuel Processing Technology. 115(0): 50-56.

Zhou, X., Ge, H., Xia, L., Zhang, D., Hu, C., 2013. Evaluation of oil-producing algae as potential biodiesel feedstock. Bioresource Technology. 134(0): 24-29.

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