Ha Thanh Toan , Tran Thi Bich Quyen * , Luong Huynh Vu Thanh , Doan Van Hong Thien , Vo Ngoc Hieu , Nguyen Thi Xuan Chi , Phan Van Hoang Khang and Nguyen Trong Tuan

* Corresponding author (ttbquyen@ctu.edu.vn)

Main Article Content

Abstract

A green and simple method has been successfully developed to synthesize chitosan/Ag nanocomposites using Kumquat extract and River-leaf creeper extract as biological reducing agents. It indicates to be an eco-friendly and green method for the synthesis providing a cost effective and an efficient route for the chitosan/Ag nanocomposites’ synthesis. The prepared chitosan/Ag nanocomposites have been characterized by UV-vis, TEM, FTIR, and XRD. Result showed those chitosan/Ag nanocomposites have been obtained with average particle size of ~15-25 nm (using kumquat extract) and ~15-41 nm (using river-leaf creeper extract). Moreover, the synthesized chitosan/Ag nanocomposites also showed their efficient antimicrobial activity against on S. aureus and E. coli. The chitosan/Ag nanocomposite was found to have significantly higher antimicrobial activity than its components at their respective concentrations. The presence of a small percentage (2.75%, w/w) of metal nanoparticles in the nanocomposite was enough to significantly enhance inactivation of S. aureus and E. coli as compared with unaltered chitosan. Thus, this eco-friendly method could be a competitive alternative to the conventional physical/chemical methods used for the synthesis of chitosan/Ag nanocompoites. Since, it has a potential to use in biomedical applications, opto-electronics and medical devices in future.
Keywords: Chitosan/silver nanocomposites (CTS/Ag NCPs), Escherichia coli bacteria, green synthesis, Kumquat extract, River-leaf creeper extract, Staphylococcus aureus bacteria

Article Details

References

Ahmed, S., Ahmad, M., Swami, B. L., and Ikram, S., 2016. A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: A green expertise. Journal of Advanced Research, 7(1): 17-28.

Ali, S. W., S. Rajendran and M. Joshi, 2011. Synthesis and characterization of chitosan and silver loaded chitosan nanoparticles for bioactive polyester. Carbohydrate Polymers, 83(2): 438-446.

Bae, C. H., Nam, S. H., and Park, S. M., 2002. Formation of silver nanoparticles by laser ablation of a silver target in NaCl solution. Applied Surface Science, 197-198: 628-634.

Badawy, M. E., and Rabea, E. I., 2011. A Biopolymer Chitosan and Its Derivatives as Promising Antimicrobial Agents against Plant Pathogens and Their Applications in Crop Protection. International Journal of Carbohydrate Chemistry, 2011(2011): 1-29.

Bar, H., Bhui, D. K., Sahoo, G. P., Sarkar, P., Pyne, S., and Misra, A., 2009. Green synthesis of silver nanoparticles using seed extract of Jatropha curcas. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 348(1-3): 212-216.

Benelli, G., 2016. Plant-mediated biosynthesis of nanoparticles as an emerging tool against mosquitoes of medical and veterinary importance: a review. Parasitology Research, 115(1): 23-34.

Chen, J., Han, C. M., Lin, X. W., Tang, Z. J., and Su, S. J., 2006. Effect of silver nanoparticle dressing on second degree burn wound. Chinese Journal of surgery, 44(1): 50-52.

Cioffi, N., Torsi, L., Ditaranto, N. et al., 2005. Copper Nanoparticle/Polymer Composites with Antifungal and Bacteriostatic Properties. Chemistry of Materials, 17(21): 5255-5262.

Du, W. L., Niu, S. S., Xu, Y. L., Xu, Z. R., and Fan, C. L., 2009. Antibacterial activity of chitosan tripolyphosphate nanoparticles loaded with various metal ions. Carbohydrate Polymers, 75(3): 385-389.

Gopinath, S. M., Saha, N.S., John, V.J., Khanum, N.S., Ganesh, S. and Patil, G.M.A, 2013. Biological Synthesis, Characterization and Application of Silver Nanoparticles. International Journal of Pharmaceutical Applications, 4(1): 19-28.

Gu, H., Ho, P. L., Tong, E., Wang, L., and Xu, B., 2003. Presenting Vancomycin on Nanoparticles to Enhance Antimicrobial Activities. Nano Letters, 3(9): 1261-1263.

Huang, Y. F., Huang, K. M., and Chang, H. T., 2006. Synthesis and characterization of Au core–Au–Ag shell nanoparticles from gold seeds: Impacts of glycine concentration and pH. Journal of Colloid and Interface Science, 301(1): 145-154.

Kharissova, O. V., Dias, H. R., Kharisov, B. I., Pérez, B. O., and Pérez, V. M. J., 2013. The greener synthesis of nanoparticles. Trends in Biotechnology, 31(4): 240-248.

Kim, Y. H., Lee, D. K., and Kang, Y. S., 2005. Synthesis and characterization of Ag and Ag–SiO2 nanoparticles. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 257-258: 273-276.

Kong, M., Chen, X. G., Xing, K., and Park, H. J., 2010. Antimicrobial properties of chitosan and mode of action: a state of the art review. International journal of food microbiology, 144(1): 51-63.

Kumar, R. and Münstedt, H., 2005. Silver ion release from antimicrobial polyamide/silver composites. Biomaterials, 26(14): 2081-2088.

Liau, S. Y., Read, D. C., Pugh, W. J., Furr, J. R., and Russell, A. D., 1997. Interaction of silver nitrate with readily identifiable groups: relationship to the antibacterialaction of silver ions. Letters in Applied Microbiology, 25(4): 279-283.

Luo, P. G. and Stutzenberger, F. J., 2008. Nanotechnology in the detection and control of microorganisms. Advances in applied microbiology, 63: 145-181.

Mittal, A. K., Chisti, Y., and Banerjee, U. C., 2013. Synthesis of metallic nanoparticles using plant extracts. Biotechnology Advances, 31(2): 346-356.

Modrzejewska, Z., Dorabialska, M., Zarzycki, R., and Wojtasz-Pająk, A. 2009. The Mechanism of sorption of Ag+ ions on chitosan microgranules: IR and NMR studies. Progress on Chemistry and Application of Chitin and its Derivatives, 14: 49- 64.

Pal, A., Shah, S., and Devi, S., 2007. Preparation of silver, gold and silver–gold bimetallic nanoparticles in w/o microemulsion containing TritonX-100. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 302(1): 483-487.

Pal, A., Shah, S. and Devi, S., 2008. Preparation of Silver–Gold Alloy Nanoparticles at Higher Concentration Using Sodium Dodecyl Sulfate. Australian Journal of Chemistry, 61(1): 66–71.

Qian, L. and Yang, X., 2005. Preparation and characterization of Ag(Au) bimetallic core–shell nanoparticles with new seed growth method. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 260(1): 79-85.

Reneker, D. H. and Yarin, A. L., 2008. Electrospinning jets and polymer nanofibers. Polymer, 49(10): 2387-2425.

Roe, D., Karandikar, B., Bonn-Savage, N., Gibbins, B., and Roullet, J. B., 2008. Antimicrobial surface functionalization of plastic catheters by silver nanoparticles. Journal of Antimicrobial Chemotherapy, 61(4): 869-876.

Panigrahi, S., Kundu, S., Ghosh, S. K., Nath, S., and Pal, T., 2005. Sugar assisted evolution of mono- and bimetallic nanoparticles. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 264(1): 133-138.

Prabhu, S. and Poulose, E. K., 2012. Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects. International Nano Letters, 2(1): 32.

Qi, L., Xu, Z., Jiang, X., Hu, C., and Zou, X., 2004. Preparation and antibacterial activity of chitosan nanoparticles. Carbohydrate Research, 339(16): 2693-2700.

Rafique, M., Sadaf, I., Rafique, M. S., and Tahir, M. B., 2017. A review on green synthesis of silver nanoparticles and their applications. Journal Artificial Cells, Nanomedicine, and Biotechnology, 45(7): 1272-1291.

Saraswathy, G., Pal, S., Rose, C., and Sastry, T. P., 2001. A novel bio-inorganic bone implant containing deglued bone, chitosan and gelatin. Bulletin of Materials Science, 24(4): 415-420.

Tang, Z., Liu, S., Dong, S. and Wang, E., 2001. Electrochemical synthesis of Ag nanoparticles on functional carbon surfaces. Journal of Electroanalytical Chemistry, 502(1-2): 146-151.

Thuesombat, P., Hannongbua, S., Akasit, S., and Chadchawan, S., 2014. Effect of silver nanoparticles on rice (Oryza sativa L. cv. KDML 105) seed germination and seedling growth. Ecotoxicology and Environmental Safety, 104: 302-309.

Tsuji, T., Kakita, T., and Tsuji, M. 2003. Preparation of nano-size particles of silver with femtosecond laser ablation in water. Applied Surface Science, 206(1): 314-320.

Wan, A. C., and Tai, B. C., 2011. CHITIN--a promising biomaterial for tissue engineering and stem cell technologies. Biotechnology Advances, 31(8): 45-79.

Webster, A., Halling, M. D., and Grant, D. M., 2007. Metal complexation of chitosan and its glutaraldehyde cross-linked derivative. Carbohydrate Research, 342(9): 1189-1201.

Zhang, J. P., Chen, P., Sun, C. H., and Hu, X. J., 2004. Sonochemical synthesis of colloidal silver catalysts for reduction of complexing silver in DTR system. Applied Catalysis A: General, 266(1): 49-54.

Most read articles by the same author(s)

1 2 3 > >>