Optimization bio-organic fertilizer production from watermelon rind with Trichoderma spp. for improved Brassica juncea growth
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Main Article Content
Abstract
This study evaluates the effectiveness of Trichoderma spp. in decomposing watermelon rind to produce bio-organic fertilizer. Response Surface Methodology (RSM) with Central Composite Design (CCD) was employed to optimize the cultivation conditions for Brassica juncea using the composted fertilizer. Results demonstrated that Trichoderma spp. significantly accelerated decomposition and improved fertilizer quality. Among the tested parameters, composting duration and fertilization rate significantly influenced plant growth, particularly leaf length and width, while microbial inoculant concentration had no notable effect. Microbial analysis confirmed that Coliform, E. coli, and Salmonella levels were below regulatory limits (ND 108/2017/ND-CP), with none detected in fertilizers produced with Trichoderma spp.. RSM optimization identified the optimal composting conditions as 18 days, 4 wt.% Trichoderma spp., and 50 v/v% fertilizer volume fraction. A high-accuracy mathematical model was developed to predict plant growth responses, effectively forecasting the impact of composting parameters on crop development. These findings highlight the potential of Trichoderma-based bio-organic fertilizers in sustainable agriculture.
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References
Babcock-Jackson, L., Konovalova, T., Krogman, J.P., Bird, R., & Díaz, L.L. (2023). Sustainable Fertilizers: Publication Landscape on Wastes as Nutrient Sources, Wastewater Treatment Processes for Nutrient Recovery, Biorefineries, and Green Ammonia Synthesis. Journal of Agricultural and Food Chemistry, 71(22), 8265-8296. https://doi.org/10.1021/acs.jafc.3c00454
Czekała, W., Dach, J., Janczak, D., Smurzyńska, A., Kwiatkowska, A., Kozłowski, K.J.J.o.W., & Development, L. (2016). Influence of maize straw content with sewage sludge on composting process. Journal of Water and Land Development, 30(1), 43-49.
https://doi.org/10.1515/jwld-2016-0020
Dung, N.T.P., Huong, L.H.L., & Phong, H.X. (2011). Isolation, selection of yeasts and determination of factors affecting watermelon wine fermentation. CTU Journal of Science, 18b, 137-145.
Feizy, J., Jahani, M., & Ahmadi, S. (2020). Antioxidant activity and mineral content of watermelon peel. Journal of Food and Bioprocess Engineering, 3(1), 35-40.
https://doi.org/10.22059/jfabe.2020.75811
Gagliardi, G.G., Borello, D., Cosentini, C., Barra Caracciolo, A., Aimola, G., Ancona, V., Ieropoulos, I.A., Garbini, G.L., Rolando, L., & Grenni, P. (2024). Microbial fuel cells with polychlorinated biphenyls contaminated soil as electrolyte: energy performance and decontamination potential in presence of compost. Journal of Power Sources, 613, 234878. https://doi.org/10.1016/j.jpowsour.2024.234878
Gathiru, M.M., Obuya, E., Noah, N.M., & Masika, E. (2024). Biosynthesized and chemically synthesized Ag/TiO2 nanocomposites: Effect of reaction parameters on synthesis using watermelon rind extract and comparative analysis. Heliyon, 10(15), e35284. https://doi.org/10.1016/j.heliyon.2024.e35284
Harman, G.E., Howell, C.R., Viterbo, A., Chet, I., & Lorito, M. (2004). Trichoderma species — opportunistic, avirulent plant symbionts. Nature Reviews Microbiology, 2(1), 43-56. https://doi.org/10.1038/nrmicro797
Karthiga, D., Chozhavendhan, S., Gandhiraj, V., & Aniskumar, M. (2022). The effects of Moringa oleifera leaf extract as an organic bio-stimulant for the growth of various plants: Review. Biocatalysis and Agricultural Biotechnology, 43, 102446. https://doi.org/10.1016/j.bcab.2022.102446
Kouniba, S., Benbiyi, A., Zourif, A., & El Guendouzi, M. (2024). Optimization use of watermelon rind in the coagulation-flocculation process by Box Behnken design for copper, zinc, and turbidity removal. Heliyon, 10(10), e30823. https://doi.org/10.1016/j.heliyon.2024.e30823
Ling Wen Xia, F., Supri, S., Djamaludin, H., Nurdiani, R., Leong Seng, L., Wee Yin, K., & Rovina, K. (2024). Turning waste into value: Extraction and effective valorization strategies of seafood by-products. Waste Management Bulletin, 2(3), 84-100. https://doi.org/10.1016/j.wmb.2024.06.008
Mahish, P.K., Verma, D.K., Ghritlahare, A., Arora, C., & Otero, P. (2024). Microbial bioconversion of food waste to bio-fertilizers. Sustainable Food Technology, 2(3), 689-708. https://doi.org/10.1039/d3fb00041a
Molina, T., Zhang, L., Nishimura, T., Johansen, S., Buenaventura, K., Wickstrom, C., & Hong, M.Y. (2023). Effects of blenderized watermelon with the rind on satiety, postprandial glucose, and bowel movement, with sensory evaluation. Human Nutrition & Metabolism, 34, 200223. https://doi.org/10.1016/j.hnm.2023.200223
Nguyen, D.T., & Tran, T.B. (2008). Research on making square watermelon for colourizing the national Vietnam’s Tet holiday. CTU Journal of Science, 9, 128-135.
Nguyen, T.T.H., Duong, M.V., & Nguyen, H.A. (2013). Investigating contamination risk of Salmonella, Shigella and E. coli on vegetables in vegetable growing areas and measures to improve. CTU Journal of Science, 25, 98-108.
Phiri, R., Mavinkere Rangappa, S., & Siengchin, S. (2024). Agro-waste for renewable and sustainable green production: A review. Journal of Cleaner Production, 434, 139989. https://doi.org/10.1016/j.jclepro.2023.139989
Shen, Z., Zhong, S., Wang, Y., Wang, B., Mei, X., Li, R., Ruan, Y., & Shen, Q. (2013). Induced soil microbial suppression of banana fusarium wilt disease using compost and biofertilizers to improve yield and quality. European Journal of Soil Biology, 57, 1-8.
https://doi.org/10.1016/j.ejsobi.2013.03.006
Tyśkiewicz, R., Nowak, A., Ozimek, E., & Jaroszuk-Ściseł, J. (2022). Trichoderma: The current status of its application in agriculture for the biocontrol of fungal phytopathogens and stimulation of plant Growth. International Journal of Molecular Sciences, 23(4), 2329. https://doi.org/10.3390/ijms23042329
Upadhyay, S.K., Singh, G., Rani, N., Rajput, V.D., Seth, C.S., Dwivedi, P., Minkina, T., Wong, M.H., Show, P.L., & Khoo, K.S. (2024). Transforming bio-waste into value-added products mediated microbes for enhancing soil health and crop production: Perspective views on circular economy. Environmental Technology & Innovation, 34, 103573. https://doi.org/10.1016/j.eti.2024.103573
Vijayan, L., Arumugam, M., Palaniyappan, S., Jayaraman, S., Brown, P.B., Kari, Z.A., Abdel-Warith, A.-W.A., Younis, E.M., & Ramasamy, T. (2024). Utilization of sustainable agri-waste watermelon rind for fishmeal in Labeo rohita diets: Effects on nutritional indices, hemato-biochemical properties, histoarchitechtural traits, amino acid and fatty acid profiles. Aquaculture Reports, 36, 102045. https://doi.org/10.1016/j.aqrep.2024.102045
Wang, F., Xie, C., Ye, R., Tang, H., Jiang, L., & Liu, Y. (2023). Development of active packaging with chitosan, guar gum and watermelon rind extract: Characterization, application and performance improvement mechanism. International Journal of Biological Macromolecules, 227, 711-725. https://doi.org/10.1016/j.ijbiomac.2022.12.210
Wang, Z., Ahmad, W., Zhu, A., Zhao, S., Ouyang, Q., & Chen, Q. (2024). Recent advances review in tea waste: High-value applications, processing technology, and value-added products. Science of The Total Environment, 946, 174225. https://doi.org/10.1016/j.scitotenv.2024.174225
Wong, J.W.C., Karthikeyan, O.P., & Selvam, A. (2017). Biological nutrient transformation during composting of pig manure and paper waste. Environmental Technology, 38(6), 754-761. https://doi.org/10.1080/09593330.2016.1211747
Xu, Z., Wang, S., Li, R., Li, H., Zhang, C., Zhang, Y., Zhang, X., Quan, F., & Wang, F. (2024). Enhancement of microbial community dynamics and metabolism in compost through ammonifying cultures inoculation. Environmental Research, 255, 119188. https://doi.org/10.1016/j.envres.2024.119188
Zhang, S., Li, Y., Jiang, L., Chen, X., Zhao, Y., Shi, W., & Xing, Z. (2024). From organic fertilizer to the soils: What happens to the microplastics? A critical review. Science of The Total Environment, 919, 170217. https://doi.org/10.1016/j.scitotenv.2024.170217
Zhou, Y., Zhang, J., Zhang, Z., Wang, P., & Xia, S. (2019). pH dependent of the waste activated sludge reduction by short-time aerobic digestion (STAD) process. Science of The Total Environment, 649, 1307-1313. https://doi.org/10.1016/j.scitotenv.2018.08.411